{"id":{"repo_id":"queens","oai_identifier":"oai:queensu.scholaris.ca:1974/8330"},"canonical_url":"https://search.dev.ndltd.org/etd/queens/oai:queensu.scholaris.ca:1974/8330","repository":{"repo_id":"queens","name":"Queens University","base_url":"https://qspace.library.queensu.ca/server/oai/request"},"display":{"title":"Elucidation of Jasmonate-Responsive Promoter Elements in the Calmodulin-Like Gene CML39 in Arabidopsis","abstract":"All organisms require rapid and flexible signaling mechanisms in order to effectively respond to biotic and abiotic stress. Calcium ions (Ca2+) have proven to be important components of signaling networks. Observations of stimulus-specific oscillations of cytosolic Ca2+ during signal transduction suggest that Ca2+ signals directly encode information. These stimulus-specific oscillations, known as Ca2+ signatures, can be interpreted by an array of Ca2+-binding sensors and effectors, which subsequently regulate appropriate cellular responses. While progress has been made regarding the classic Ca2+-sensor calmodulin (CaM), less research has been directed towards the CaM-like family of Ca2+-sensors (CMLs). This family – unique to plants – is suspected to regulate a multitude of stress and developmental pathways; however, to date very few members of this family have had their functions elucidated by the identification of downstream targets and upstream regulators. In the present study, I investigate the regulation of CML39, which has previously been shown to strongly respond to the stress hormone jasmonic acid (JA) in Arabidopsis. Bioinformatic tools predict a large number of putative JA-responsive cis-elements within the CML39 promoter. Deletion analysis of CML39 promoter fragments in planta reveals that some cis-elements respond in a tissue-specific manner. Analysis of transgenic MYC2 loss-of-function (myc2) mutants demonstrates that MYC2 – the preeminent JA-responsive transcription factor – is not necessary for CML39 promoter activity. Collectively, these data reveal a complex tissue-specific pattern of CML39 regulation and provide a foundation for the future identification of relevant transcription factors.","abstract_html":"All organisms require rapid and flexible signaling mechanisms in order to effectively respond to biotic and abiotic stress. Calcium ions (Ca2+) have proven to be important components of signaling networks. Observations of stimulus-specific oscillations of cytosolic Ca2+ during signal transduction suggest that Ca2+ signals directly encode information. These stimulus-specific oscillations, known as Ca2+ signatures, can be interpreted by an array of Ca2+-binding sensors and effectors, which subsequently regulate appropriate cellular responses. While progress has been made regarding the classic Ca2+-sensor calmodulin (CaM), less research has been directed towards the CaM-like family of Ca2+-sensors (CMLs). This family – unique to plants – is suspected to regulate a multitude of stress and developmental pathways; however, to date very few members of this family have had their functions elucidated by the identification of downstream targets and upstream regulators. In the present study, I investigate the regulation of CML39, which has previously been shown to strongly respond to the stress hormone jasmonic acid (JA) in Arabidopsis. Bioinformatic tools predict a large number of putative JA-responsive cis-elements within the CML39 promoter. Deletion analysis of CML39 promoter fragments in planta reveals that some cis-elements respond in a tissue-specific manner. Analysis of transgenic MYC2 loss-of-function (myc2) mutants demonstrates that MYC2 – the preeminent JA-responsive transcription factor – is not necessary for CML39 promoter activity. Collectively, these data reveal a complex tissue-specific pattern of CML39 regulation and provide a foundation for the future identification of relevant transcription factors.","abstract_has_math":false,"creators":["Maj, David"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":"Biology","school":null,"contributors":[],"advisors":["Snedden, Wayne A."],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-09-27","date_published":"2013-09-27","updated_at":"2026-07-27T20:35:25Z","subjects":["Calcium Signaling","Calmodulin-Like","Cis-Element","Promoter","Jasmonic Acid"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/1974/8330","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.department","label":"Department","values":["Biology"]},{"key":"dc:contributor.supervisor","label":"Supervisor","values":["Snedden, Wayne A."]},{"key":"dc:creator","label":"Author","values":["Maj, David"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2013-09-24 14:54:13.878","2013-09-24 21:06:30.592"]},{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2013-09-27T19:01:18Z"]},{"key":"dc:date.issued","label":"Date","values":["2013-09-27"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Calcium Signaling","Calmodulin-Like","Cis-Element","Promoter","Jasmonic Acid"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/1974/8330"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Thesis (Master, Biology) -- Queen's University, 2013-09-24 21:06:30.592"]},{"key":"dc:description.abstract","label":"Abstract","values":["All organisms require rapid and flexible signaling mechanisms in order to effectively respond to biotic and abiotic stress. Calcium ions (Ca2+) have proven to be important components of signaling networks. Observations of stimulus-specific oscillations of cytosolic Ca2+ during signal transduction suggest that Ca2+ signals directly encode information. These stimulus-specific oscillations, known as Ca2+ signatures, can be interpreted by an array of Ca2+-binding sensors and effectors, which subsequently regulate appropriate cellular responses. While progress has been made regarding the classic Ca2+-sensor calmodulin (CaM), less research has been directed towards the CaM-like family of Ca2+-sensors (CMLs). This family – unique to plants – is suspected to regulate a multitude of stress and developmental pathways; however, to date very few members of this family have had their functions elucidated by the identification of downstream targets and upstream regulators. In the present study, I investigate the regulation of CML39, which has previously been shown to strongly respond to the stress hormone jasmonic acid (JA) in Arabidopsis. Bioinformatic tools predict a large number of putative JA-responsive cis-elements within the CML39 promoter. Deletion analysis of CML39 promoter fragments in planta reveals that some cis-elements respond in a tissue-specific manner. Analysis of transgenic MYC2 loss-of-function (myc2) mutants demonstrates that MYC2 – the preeminent JA-responsive transcription factor – is not necessary for CML39 promoter activity. Collectively, these data reveal a complex tissue-specific pattern of CML39 regulation and provide a foundation for the future identification of relevant transcription factors."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["M.Sc."]},{"key":"dc:title","label":"Title","values":["Elucidation of Jasmonate-Responsive Promoter Elements in the Calmodulin-Like Gene CML39 in Arabidopsis"]}]}],"canonical_facts":{"dc:contributor.department":["Biology"],"dc:contributor.supervisor":["Snedden, Wayne A."],"dc:creator":["Maj, David"],"dc:date":["2013-09-24 14:54:13.878","2013-09-24 21:06:30.592"],"dc:date.accessioned":["2013-09-27T19:01:18Z"],"dc:date.issued":["2013-09-27"],"dc:description":["Thesis (Master, Biology) -- Queen's University, 2013-09-24 21:06:30.592"],"dc:description.abstract":["All organisms require rapid and flexible signaling mechanisms in order to effectively respond to biotic and abiotic stress. Calcium ions (Ca2+) have proven to be important components of signaling networks. Observations of stimulus-specific oscillations of cytosolic Ca2+ during signal transduction suggest that Ca2+ signals directly encode information. These stimulus-specific oscillations, known as Ca2+ signatures, can be interpreted by an array of Ca2+-binding sensors and effectors, which subsequently regulate appropriate cellular responses. While progress has been made regarding the classic Ca2+-sensor calmodulin (CaM), less research has been directed towards the CaM-like family of Ca2+-sensors (CMLs). This family – unique to plants – is suspected to regulate a multitude of stress and developmental pathways; however, to date very few members of this family have had their functions elucidated by the identification of downstream targets and upstream regulators. In the present study, I investigate the regulation of CML39, which has previously been shown to strongly respond to the stress hormone jasmonic acid (JA) in Arabidopsis. Bioinformatic tools predict a large number of putative JA-responsive cis-elements within the CML39 promoter. Deletion analysis of CML39 promoter fragments in planta reveals that some cis-elements respond in a tissue-specific manner. Analysis of transgenic MYC2 loss-of-function (myc2) mutants demonstrates that MYC2 – the preeminent JA-responsive transcription factor – is not necessary for CML39 promoter activity. Collectively, these data reveal a complex tissue-specific pattern of CML39 regulation and provide a foundation for the future identification of relevant transcription factors."],"dc:description.degree":["M.Sc."],"dc:identifier.uri":["http://hdl.handle.net/1974/8330"],"dc:language.iso":["eng"],"dc:subject":["Calcium Signaling","Calmodulin-Like","Cis-Element","Promoter","Jasmonic Acid"],"dc:title":["Elucidation of Jasmonate-Responsive Promoter Elements in the Calmodulin-Like Gene CML39 in Arabidopsis"],"dc:type":["thesis"]},"updated_at":"2026-07-27T20:35:25Z"}