{"id":{"repo_id":"qu-belfast","oai_identifier":"oai:pure.qub.ac.uk/portal:studenttheses/fff701af-d203-4ba3-b04c-951e87710f2d"},"canonical_url":"https://search.dev.ndltd.org/etd/qu-belfast/oai:pure.qub.ac.uk/portal:studenttheses/fff701af-d203-4ba3-b04c-951e87710f2d","repository":{"repo_id":"qu-belfast","name":"Queen's University Belfast","base_url":"https://pureadmin.qub.ac.uk/ws/oai"},"display":{"title":"Identification and activity assessment of a bioactive peptide QUB-1368 from the skin secretion of the American bullfrog (Rana catesbeiana)","abstract":"Polypeptides in amphibian skin secretions have various bioactive functions, especially the positively-charged, alpha-helical and amphipathic polypeptides. In this study, a novel antimicrobial peptide isolated from the skin secretions of Rana catesbeiana was named QUB-1368. After establishing the cDNA library, the biosynthetic precursor was obtained through molecular cloning, and then the mature peptide QUB-1368 was deduced from the precursor. QUB-1368 was then obtained in quantity by solid-phase peptide synthesis for biological activity experiments. The purified synthetic peptide QUB-1368 was identified by reversed-phase high-performance liquid chromatography (HPLC) and Matrix-Assisted Laser Desorption/ Ionization Time of Flight Mass Spectrometry (MALDI-TOF). In this study, QUB-1368 showed a broad-spectrum antibacterial activity to Gram-negative bacteria (64 µM against E. coli ATCC CRM 8739), Gram-positive bacteria (32 µM against S. aureus ATCC CRM 6538) and yeast (64 µM against C. albicans ATCC CRM 10231). In addition, in the concentration range of 102-10-3 µM, QUB-1368 has no inhibitory effect on the human lung cancer cell line (H838). In the haemolysis assay, QUB-1368 showed haemolytic activity at a concentration of 256 µM, when QUB-1368 showed antibacterial activity, the concentration did not reach to produce hemolysis. In summary, QUB-1368 may have good clinical research value.<br/><br/><i>Thesis embargoed until 31 December 2026</i>.","abstract_html":"Polypeptides in amphibian skin secretions have various bioactive functions, especially the positively-charged, alpha-helical and amphipathic polypeptides. In this study, a novel antimicrobial peptide isolated from the skin secretions of Rana catesbeiana was named QUB-1368. After establishing the cDNA library, the biosynthetic precursor was obtained through molecular cloning, and then the mature peptide QUB-1368 was deduced from the precursor. QUB-1368 was then obtained in quantity by solid-phase peptide synthesis for biological activity experiments. The purified synthetic peptide QUB-1368 was identified by reversed-phase high-performance liquid chromatography (HPLC) and Matrix-Assisted Laser Desorption/ Ionization Time of Flight Mass Spectrometry (MALDI-TOF). In this study, QUB-1368 showed a broad-spectrum antibacterial activity to Gram-negative bacteria (64 µM against E. coli ATCC CRM 8739), Gram-positive bacteria (32 µM against S. aureus ATCC CRM 6538) and yeast (64 µM against C. albicans ATCC CRM 10231). In addition, in the concentration range of 102-10-3 µM, QUB-1368 has no inhibitory effect on the human lung cancer cell line (H838). In the haemolysis assay, QUB-1368 showed haemolytic activity at a concentration of 256 µM, when QUB-1368 showed antibacterial activity, the concentration did not reach to produce hemolysis. In summary, QUB-1368 may have good clinical research value.&lt;br/&gt;&lt;br/&gt;&lt;i&gt;Thesis embargoed until 31 December 2026&lt;/i&gt;.","abstract_has_math":false,"creators":["Lin, Huishu"],"institution":"Queen's University Belfast","degree_name":"Master of Philosophy","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Zhou, Mei","Wang, Lei","Chen, Tianbao"],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-12","date_published":"2021-12","updated_at":"2026-07-24T03:56:04Z","subjects":["Antimicrobial peptides","bioactivity evaluation","antimicrobial activity","haemolytic activity","temporin peptides"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/fff701af-d203-4ba3-b04c-951e87710f2d"],"render_values":[{"text":"oai:pure.qub.ac.uk/portal:studenttheses/fff701af-d203-4ba3-b04c-951e87710f2d","href":null,"code":true}]}]},"links":{"outbound_url":"https://pure.qub.ac.uk/en/studentTheses/fff701af-d203-4ba3-b04c-951e87710f2d","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Zhou, Mei","Wang, Lei","Chen, Tianbao"]},{"key":"dc:creator","label":"Author","values":["Lin, Huishu"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2021-12"]},{"key":"dc:date.issued","label":"Date","values":["2021-12"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Pharmacy"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Queen's University Belfast"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://pure.qub.ac.uk/en/studentTheses/fff701af-d203-4ba3-b04c-951e87710f2d"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Master of Philosophy"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Antimicrobial peptides","bioactivity evaluation","antimicrobial activity","haemolytic activity","temporin peptides"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights.embargodate","label":"Dc Rights Embargodate","values":["2026-12-31"]},{"key":"dc:rights.embargoreason","label":"Dc Rights Embargoreason","values":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/fff701af-d203-4ba3-b04c-951e87710f2d","https://pure.qub.ac.uk/en/studentTheses/fff701af-d203-4ba3-b04c-951e87710f2d"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Polypeptides in amphibian skin secretions have various bioactive functions, especially the positively-charged, alpha-helical and amphipathic polypeptides. In this study, a novel antimicrobial peptide isolated from the skin secretions of Rana catesbeiana was named QUB-1368. After establishing the cDNA library, the biosynthetic precursor was obtained through molecular cloning, and then the mature peptide QUB-1368 was deduced from the precursor. QUB-1368 was then obtained in quantity by solid-phase peptide synthesis for biological activity experiments. The purified synthetic peptide QUB-1368 was identified by reversed-phase high-performance liquid chromatography (HPLC) and Matrix-Assisted Laser Desorption/ Ionization Time of Flight Mass Spectrometry (MALDI-TOF). In this study, QUB-1368 showed a broad-spectrum antibacterial activity to Gram-negative bacteria (64 µM against E. coli ATCC CRM 8739), Gram-positive bacteria (32 µM against S. aureus ATCC CRM 6538) and yeast (64 µM against C. albicans ATCC CRM 10231). In addition, in the concentration range of 102-10-3 µM, QUB-1368 has no inhibitory effect on the human lung cancer cell line (H838). In the haemolysis assay, QUB-1368 showed haemolytic activity at a concentration of 256 µM, when QUB-1368 showed antibacterial activity, the concentration did not reach to produce hemolysis. In summary, QUB-1368 may have good clinical research value.<br/><br/><i>Thesis embargoed until 31 December 2026</i>."]},{"key":"dc:title","label":"Title","values":["Identification and activity assessment of a bioactive peptide QUB-1368 from the skin secretion of the American bullfrog (Rana catesbeiana)"]}]}],"canonical_facts":{"dc:contributor.advisor":["Zhou, Mei","Wang, Lei","Chen, Tianbao"],"dc:creator":["Lin, Huishu"],"dc:date":["2021-12"],"dc:date.issued":["2021-12"],"dc:description.abstract":["Polypeptides in amphibian skin secretions have various bioactive functions, especially the positively-charged, alpha-helical and amphipathic polypeptides. In this study, a novel antimicrobial peptide isolated from the skin secretions of Rana catesbeiana was named QUB-1368. After establishing the cDNA library, the biosynthetic precursor was obtained through molecular cloning, and then the mature peptide QUB-1368 was deduced from the precursor. QUB-1368 was then obtained in quantity by solid-phase peptide synthesis for biological activity experiments. The purified synthetic peptide QUB-1368 was identified by reversed-phase high-performance liquid chromatography (HPLC) and Matrix-Assisted Laser Desorption/ Ionization Time of Flight Mass Spectrometry (MALDI-TOF). In this study, QUB-1368 showed a broad-spectrum antibacterial activity to Gram-negative bacteria (64 µM against E. coli ATCC CRM 8739), Gram-positive bacteria (32 µM against S. aureus ATCC CRM 6538) and yeast (64 µM against C. albicans ATCC CRM 10231). In addition, in the concentration range of 102-10-3 µM, QUB-1368 has no inhibitory effect on the human lung cancer cell line (H838). In the haemolysis assay, QUB-1368 showed haemolytic activity at a concentration of 256 µM, when QUB-1368 showed antibacterial activity, the concentration did not reach to produce hemolysis. In summary, QUB-1368 may have good clinical research value.<br/><br/><i>Thesis embargoed until 31 December 2026</i>."],"dc:identifier":["oai:pure.qub.ac.uk/portal:studenttheses/fff701af-d203-4ba3-b04c-951e87710f2d","https://pure.qub.ac.uk/en/studentTheses/fff701af-d203-4ba3-b04c-951e87710f2d"],"dc:language":["eng"],"dc:publisher.department":["School of Pharmacy"],"dc:publisher.institution":["Queen's University Belfast"],"dc:relation.isreferencedby":["https://pure.qub.ac.uk/en/studentTheses/fff701af-d203-4ba3-b04c-951e87710f2d"],"dc:rights.embargodate":["2026-12-31"],"dc:rights.embargoreason":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"],"dc:subject":["Antimicrobial peptides","bioactivity evaluation","antimicrobial activity","haemolytic activity","temporin peptides"],"dc:title":["Identification and activity assessment of a bioactive peptide QUB-1368 from the skin secretion of the American bullfrog (Rana catesbeiana)"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["Masters Thesis"],"dc:type.qualificationname":["Master of Philosophy"]},"updated_at":"2026-07-24T03:56:04Z"}