{"id":{"repo_id":"qu-belfast","oai_identifier":"oai:pure.qub.ac.uk/portal:studenttheses/d1ead41c-acc5-419c-8636-529f4aa6942f"},"canonical_url":"https://search.dev.ndltd.org/etd/qu-belfast/oai:pure.qub.ac.uk/portal:studenttheses/d1ead41c-acc5-419c-8636-529f4aa6942f","repository":{"repo_id":"qu-belfast","name":"Queen's University Belfast","base_url":"https://pureadmin.qub.ac.uk/ws/oai"},"display":{"title":"Discovery and bioactivity assessment of a novel peptide from the skin secretion of the Senegal running frog Kassina senegalens","abstract":"Frog skin is characterised with an abundance of biologically active antimicrobial peptides (AMPs) with a broad-spectrum effect against a variety of microorganisms. The contribution of their complex and diverse structures, physicochemical properties and mechanisms of biological activity, have been discovered and widely reported. In this study, a novel peptide named QUB-1923 was identified from the skin secretion of the Senegal running frog, Kassina senegalensis. The sequence of the mature peptide (FYHLSALIHGIKQRLK) was deduced from the cloned cDNA encoding a prepropeptide. Subsequently, this peptide was chemically synthesised by solid-phase synthesis. Following on from the purification by RP-HPLC and MALDI-TOF MS, the antimicrobial, anti-cancer cell proliferative and haemolytic activities of the purified peptide QUB-1923 were investigated. Results from antimicrobial assays have shown that this peptide has a broad-spectrum antimicrobial effect on S. aureus, E. coli and C. albicans. Notably, this peptide displayed the highest antimicrobial activity against E. coli (MIC=4 μM, and MBC=8 μM). On the other hand, this peptide only caused approximately 20% growth inhibition on lung cancer cell line H838 at a concentration of 100 μM. Additionally, this peptide demonstrated less than 10% haemolysis at a concentration of 256µM. These results suggest that this novel peptide is worthy of attention as a new antibacterial lead due to its potent inhibitory and bactericidal effects against Gram-ve bacteria, along with a low level of haemolysis.<br/><i><br/>Thesis embargoed until 31 December 2026</i>.","abstract_html":"Frog skin is characterised with an abundance of biologically active antimicrobial peptides (AMPs) with a broad-spectrum effect against a variety of microorganisms. The contribution of their complex and diverse structures, physicochemical properties and mechanisms of biological activity, have been discovered and widely reported. In this study, a novel peptide named QUB-1923 was identified from the skin secretion of the Senegal running frog, Kassina senegalensis. The sequence of the mature peptide (FYHLSALIHGIKQRLK) was deduced from the cloned cDNA encoding a prepropeptide. Subsequently, this peptide was chemically synthesised by solid-phase synthesis. Following on from the purification by RP-HPLC and MALDI-TOF MS, the antimicrobial, anti-cancer cell proliferative and haemolytic activities of the purified peptide QUB-1923 were investigated. Results from antimicrobial assays have shown that this peptide has a broad-spectrum antimicrobial effect on S. aureus, E. coli and C. albicans. Notably, this peptide displayed the highest antimicrobial activity against E. coli (MIC=4 μM, and MBC=8 μM). On the other hand, this peptide only caused approximately 20% growth inhibition on lung cancer cell line H838 at a concentration of 100 μM. Additionally, this peptide demonstrated less than 10% haemolysis at a concentration of 256µM. These results suggest that this novel peptide is worthy of attention as a new antibacterial lead due to its potent inhibitory and bactericidal effects against Gram-ve bacteria, along with a low level of haemolysis.&lt;br/&gt;&lt;i&gt;&lt;br/&gt;Thesis embargoed until 31 December 2026&lt;/i&gt;.","abstract_has_math":false,"creators":["Zhou, Chunyuan"],"institution":"Queen's University Belfast","degree_name":"Master of Philosophy","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Zhou, Mei","Wang, Lei","Chen, Tianbao","Chen, Xiaoling"],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-12","date_published":"2021-12","updated_at":"2026-07-24T03:56:04Z","subjects":["Antimicrobial peptide","bioactivity assessment","broad-spectrum antimicrobial activity","low haemolysis activity","novel peptide"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/d1ead41c-acc5-419c-8636-529f4aa6942f"],"render_values":[{"text":"oai:pure.qub.ac.uk/portal:studenttheses/d1ead41c-acc5-419c-8636-529f4aa6942f","href":null,"code":true}]}]},"links":{"outbound_url":"https://pure.qub.ac.uk/en/studentTheses/d1ead41c-acc5-419c-8636-529f4aa6942f","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Zhou, Mei","Wang, Lei","Chen, Tianbao","Chen, Xiaoling"]},{"key":"dc:creator","label":"Author","values":["Zhou, Chunyuan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2021-12"]},{"key":"dc:date.issued","label":"Date","values":["2021-12"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Pharmacy"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Queen's University Belfast"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://pure.qub.ac.uk/en/studentTheses/d1ead41c-acc5-419c-8636-529f4aa6942f"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Master of Philosophy"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Antimicrobial peptide","bioactivity assessment","broad-spectrum antimicrobial activity","low haemolysis activity","novel peptide"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights.embargodate","label":"Dc Rights Embargodate","values":["2026-12-31"]},{"key":"dc:rights.embargoreason","label":"Dc Rights Embargoreason","values":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/d1ead41c-acc5-419c-8636-529f4aa6942f","https://pure.qub.ac.uk/en/studentTheses/d1ead41c-acc5-419c-8636-529f4aa6942f"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Frog skin is characterised with an abundance of biologically active antimicrobial peptides (AMPs) with a broad-spectrum effect against a variety of microorganisms. The contribution of their complex and diverse structures, physicochemical properties and mechanisms of biological activity, have been discovered and widely reported. In this study, a novel peptide named QUB-1923 was identified from the skin secretion of the Senegal running frog, Kassina senegalensis. The sequence of the mature peptide (FYHLSALIHGIKQRLK) was deduced from the cloned cDNA encoding a prepropeptide. Subsequently, this peptide was chemically synthesised by solid-phase synthesis. Following on from the purification by RP-HPLC and MALDI-TOF MS, the antimicrobial, anti-cancer cell proliferative and haemolytic activities of the purified peptide QUB-1923 were investigated. Results from antimicrobial assays have shown that this peptide has a broad-spectrum antimicrobial effect on S. aureus, E. coli and C. albicans. Notably, this peptide displayed the highest antimicrobial activity against E. coli (MIC=4 μM, and MBC=8 μM). On the other hand, this peptide only caused approximately 20% growth inhibition on lung cancer cell line H838 at a concentration of 100 μM. Additionally, this peptide demonstrated less than 10% haemolysis at a concentration of 256µM. These results suggest that this novel peptide is worthy of attention as a new antibacterial lead due to its potent inhibitory and bactericidal effects against Gram-ve bacteria, along with a low level of haemolysis.<br/><i><br/>Thesis embargoed until 31 December 2026</i>."]},{"key":"dc:title","label":"Title","values":["Discovery and bioactivity assessment of a novel peptide from the skin secretion of the Senegal running frog Kassina senegalens"]}]}],"canonical_facts":{"dc:contributor.advisor":["Zhou, Mei","Wang, Lei","Chen, Tianbao","Chen, Xiaoling"],"dc:creator":["Zhou, Chunyuan"],"dc:date":["2021-12"],"dc:date.issued":["2021-12"],"dc:description.abstract":["Frog skin is characterised with an abundance of biologically active antimicrobial peptides (AMPs) with a broad-spectrum effect against a variety of microorganisms. The contribution of their complex and diverse structures, physicochemical properties and mechanisms of biological activity, have been discovered and widely reported. In this study, a novel peptide named QUB-1923 was identified from the skin secretion of the Senegal running frog, Kassina senegalensis. The sequence of the mature peptide (FYHLSALIHGIKQRLK) was deduced from the cloned cDNA encoding a prepropeptide. Subsequently, this peptide was chemically synthesised by solid-phase synthesis. Following on from the purification by RP-HPLC and MALDI-TOF MS, the antimicrobial, anti-cancer cell proliferative and haemolytic activities of the purified peptide QUB-1923 were investigated. Results from antimicrobial assays have shown that this peptide has a broad-spectrum antimicrobial effect on S. aureus, E. coli and C. albicans. Notably, this peptide displayed the highest antimicrobial activity against E. coli (MIC=4 μM, and MBC=8 μM). On the other hand, this peptide only caused approximately 20% growth inhibition on lung cancer cell line H838 at a concentration of 100 μM. Additionally, this peptide demonstrated less than 10% haemolysis at a concentration of 256µM. These results suggest that this novel peptide is worthy of attention as a new antibacterial lead due to its potent inhibitory and bactericidal effects against Gram-ve bacteria, along with a low level of haemolysis.<br/><i><br/>Thesis embargoed until 31 December 2026</i>."],"dc:identifier":["oai:pure.qub.ac.uk/portal:studenttheses/d1ead41c-acc5-419c-8636-529f4aa6942f","https://pure.qub.ac.uk/en/studentTheses/d1ead41c-acc5-419c-8636-529f4aa6942f"],"dc:language":["eng"],"dc:publisher.department":["School of Pharmacy"],"dc:publisher.institution":["Queen's University Belfast"],"dc:relation.isreferencedby":["https://pure.qub.ac.uk/en/studentTheses/d1ead41c-acc5-419c-8636-529f4aa6942f"],"dc:rights.embargodate":["2026-12-31"],"dc:rights.embargoreason":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"],"dc:subject":["Antimicrobial peptide","bioactivity assessment","broad-spectrum antimicrobial activity","low haemolysis activity","novel peptide"],"dc:title":["Discovery and bioactivity assessment of a novel peptide from the skin secretion of the Senegal running frog Kassina senegalens"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["Masters Thesis"],"dc:type.qualificationname":["Master of Philosophy"]},"updated_at":"2026-07-24T03:56:04Z"}