{"id":{"repo_id":"qu-belfast","oai_identifier":"oai:pure.qub.ac.uk/portal:studenttheses/6bce24e7-999f-4d29-895b-dee0ec8eb59e"},"canonical_url":"https://search.dev.ndltd.org/etd/qu-belfast/oai:pure.qub.ac.uk/portal:studenttheses/6bce24e7-999f-4d29-895b-dee0ec8eb59e","repository":{"repo_id":"qu-belfast","name":"Queen's University Belfast","base_url":"https://pureadmin.qub.ac.uk/ws/oai"},"display":{"title":"A bioactivity peptide QUB2852 from the frog skin secretion of pelophylax nigromaculata","abstract":"Due to the resistance of antibiotics, antimicrobial peptides (AMPs) become research hotspot. Majority of AMPs can disrupt the cell membrane of microorganisms, leading to the death of microorganisms. In this study, the AMP QUB-2852 was identified from the skin secretion of pelophylax nigromaculata through cloning of its precursor-encoding cDNA from a skin secretion-derived cDNA library by using a 3'-rapid amplification of cDNA ends (RACE) “shotgun” strategy. Subsequently, the mature peptide sequence was confirmed through online alignment analysis. Biological assessment using the chemically synthesised replicates of QUB-2852 showed that it possessed potent antimicrobial activity against Gram-positive Bacteria Staphylococcus aureus at 32 μmol/l However, QUB-2852 showed relatively weaker inhibitory activity against the Gram-negative bacterial and yeast. In addition, QUB-2852 exhibited anti-proliferative activity against human cancer cell line U251MG. The haemolysis effect was relatively low, which was less than 20% at the concentration up to 512 μmol/l. These characteristics make QUB-2852 a promising antimicrobial agent that warrants further investigations. In particular, its structure-activity relationship would provide valuable insights into drug design for novel antimicrobial and/or anti-cancer agents development.","abstract_html":"Due to the resistance of antibiotics, antimicrobial peptides (AMPs) become research hotspot. Majority of AMPs can disrupt the cell membrane of microorganisms, leading to the death of microorganisms. In this study, the AMP QUB-2852 was identified from the skin secretion of pelophylax nigromaculata through cloning of its precursor-encoding cDNA from a skin secretion-derived cDNA library by using a 3'-rapid amplification of cDNA ends (RACE) “shotgun” strategy. Subsequently, the mature peptide sequence was confirmed through online alignment analysis. Biological assessment using the chemically synthesised replicates of QUB-2852 showed that it possessed potent antimicrobial activity against Gram-positive Bacteria Staphylococcus aureus at 32 μmol/l However, QUB-2852 showed relatively weaker inhibitory activity against the Gram-negative bacterial and yeast. In addition, QUB-2852 exhibited anti-proliferative activity against human cancer cell line U251MG. The haemolysis effect was relatively low, which was less than 20% at the concentration up to 512 μmol/l. These characteristics make QUB-2852 a promising antimicrobial agent that warrants further investigations. In particular, its structure-activity relationship would provide valuable insights into drug design for novel antimicrobial and/or anti-cancer agents development.","abstract_has_math":false,"creators":["Wang, Yangchen"],"institution":"Queen's University Belfast","degree_name":"Master of Philosophy","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Chen, Tianbao","Shaw, Christopher","Zhou, Mei","Wang, Lei"],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-7","date_published":"2018-7","updated_at":"2026-07-24T03:54:53Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/6bce24e7-999f-4d29-895b-dee0ec8eb59e"],"render_values":[{"text":"oai:pure.qub.ac.uk/portal:studenttheses/6bce24e7-999f-4d29-895b-dee0ec8eb59e","href":null,"code":true}]}]},"links":{"outbound_url":"https://pure.qub.ac.uk/en/studentTheses/6bce24e7-999f-4d29-895b-dee0ec8eb59e","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Chen, Tianbao","Shaw, Christopher","Zhou, Mei","Wang, Lei"]},{"key":"dc:creator","label":"Author","values":["Wang, Yangchen"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2018-7"]},{"key":"dc:date.issued","label":"Date","values":["2018-7"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Pharmacy"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Queen's University Belfast"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://pure.qub.ac.uk/en/studentTheses/6bce24e7-999f-4d29-895b-dee0ec8eb59e"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Master of Philosophy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights.embargodate","label":"Dc Rights Embargodate","values":["2023-10-31"]},{"key":"dc:rights.embargoreason","label":"Dc Rights Embargoreason","values":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/6bce24e7-999f-4d29-895b-dee0ec8eb59e","https://pure.qub.ac.uk/en/studentTheses/6bce24e7-999f-4d29-895b-dee0ec8eb59e"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://pure.qub.ac.uk/files/158080196/Wang_Yangchen_Thesis_final_edition.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Due to the resistance of antibiotics, antimicrobial peptides (AMPs) become research hotspot. Majority of AMPs can disrupt the cell membrane of microorganisms, leading to the death of microorganisms. In this study, the AMP QUB-2852 was identified from the skin secretion of pelophylax nigromaculata through cloning of its precursor-encoding cDNA from a skin secretion-derived cDNA library by using a 3'-rapid amplification of cDNA ends (RACE) “shotgun” strategy. Subsequently, the mature peptide sequence was confirmed through online alignment analysis. Biological assessment using the chemically synthesised replicates of QUB-2852 showed that it possessed potent antimicrobial activity against Gram-positive Bacteria Staphylococcus aureus at 32 μmol/l However, QUB-2852 showed relatively weaker inhibitory activity against the Gram-negative bacterial and yeast. In addition, QUB-2852 exhibited anti-proliferative activity against human cancer cell line U251MG. The haemolysis effect was relatively low, which was less than 20% at the concentration up to 512 μmol/l. These characteristics make QUB-2852 a promising antimicrobial agent that warrants further investigations. In particular, its structure-activity relationship would provide valuable insights into drug design for novel antimicrobial and/or anti-cancer agents development."]},{"key":"dc:title","label":"Title","values":["A bioactivity peptide QUB2852 from the frog skin secretion of pelophylax nigromaculata"]}]}],"canonical_facts":{"dc:contributor.advisor":["Chen, Tianbao","Shaw, Christopher","Zhou, Mei","Wang, Lei"],"dc:creator":["Wang, Yangchen"],"dc:date":["2018-7"],"dc:date.issued":["2018-7"],"dc:description.abstract":["Due to the resistance of antibiotics, antimicrobial peptides (AMPs) become research hotspot. Majority of AMPs can disrupt the cell membrane of microorganisms, leading to the death of microorganisms. 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