A bioactive peptide from the skin secretion of the Chinese Torrent Frog, Amolops wuyiensis

Natural products, as rich and varied resources for the discovery of novel drugs, have been essential for human healthcare systems throughout history. A variety of bioactive components with unique structures and functions which isolated from the skin secretion, especially the peptides, have enormous potential for medical purposes. <br/>Here, a Kunitz-type peptide was identified from the Chinese Torrent Frog, Amolops wuyiensis by molecular cloning. Then the mature peptide was successfully synthesised by Tribute® 2-channel peptide synthesiser. Reversed-phase high performance liquid chromatography (RP-HPLC) was used for purification and matrix-assisted laser desorption ionisation time-of-flight (MALDI-TOF) MS was used to confirm its molecular mass. This peptide was found to show relatively strong trypsin inhibition with a Ki value of 2.331 µM with weak hemolysis at a concentration up to 512μM. The peptide was ineffective against the gram-positive bacterium S.aureus, gram-negative bacterium E.coli and pathogenic yeast C.albicans. Thus, QUB-1402 could expand the potential drug discovery and development pipeline directed towards alleviation of serine protease-mediated pathologies.<br/>

Name dc:type.qualificationname

Master of Philosophy

Level dc:type.qualificationlevel

Masters Thesis

Grantor dc:publisher.institution

Queen's University Belfast

Year dc:date.issued

2018

Author dc:creator

• Huang, Ziyan

Advisors dc:contributor.advisor

• Zhou, Mei
• Wang, Lei
• Ma, Chengbang

Language dc:language

eng

Identifier

oai:pure.qub.ac.uk/portal:studenttheses/67f04197-f4e3-450f-b137-78e1071d5ccc

OAI identifier oai:identifier

oai:pure.qub.ac.uk/portal:studenttheses/67f04197-f4e3-450f-b137-78e1071d5ccc