{"id":{"repo_id":"qu-belfast","oai_identifier":"oai:pure.qub.ac.uk/portal:studenttheses/629396ea-5c91-4106-8860-aca9a090e1ac"},"canonical_url":"https://search.dev.ndltd.org/etd/qu-belfast/oai:pure.qub.ac.uk/portal:studenttheses/629396ea-5c91-4106-8860-aca9a090e1ac","repository":{"repo_id":"qu-belfast","name":"Queen's University Belfast","base_url":"https://pureadmin.qub.ac.uk/ws/oai"},"display":{"title":"A bioactive peptide from the skin of the Chinese Torrent Frog, <i>Amolops wuyiensis</i>","abstract":"Peptides derived from frog skin secretion have great potential as drug leads as they exhibit many bioactive functions. In this study, the precursor peptide of Lividin-AW was identified from the skin secretion of the Chinese torrent frog (Amolops wuyiensis) by using ‘shotgun’ cloning and its encoded mature peptide, sequenced as AVPLIYNRPGVYVTKRPK-NH2, was synthesised by Fmoc-solid phase peptide synthesis. The structure of the synthetic peptide was confirmed by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry and purified by reversed-phase high performance liquid chromatography (RP-HPLC). To determine the bioactive function of Lividin-AW, a series of bioactivity assays, including antimicrobial assays, cell viability assays, a trypsin inhibition assay and a haemolysis assay, were conducted and the results showed that Lividin-AW exhibited weak antimicrobial activity against Candida albicans (MIC=128µM), Staphylococcus aureus (MIC=512 µM) and Escherichia coli (MIC=512 µM), with negligible haemolytic activity, but neither trypsin inhibition activity nor anticancer activity were detected.","abstract_html":"Peptides derived from frog skin secretion have great potential as drug leads as they exhibit many bioactive functions. In this study, the precursor peptide of Lividin-AW was identified from the skin secretion of the Chinese torrent frog (Amolops wuyiensis) by using ‘shotgun’ cloning and its encoded mature peptide, sequenced as AVPLIYNRPGVYVTKRPK-NH2, was synthesised by Fmoc-solid phase peptide synthesis. The structure of the synthetic peptide was confirmed by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry and purified by reversed-phase high performance liquid chromatography (RP-HPLC). To determine the bioactive function of Lividin-AW, a series of bioactivity assays, including antimicrobial assays, cell viability assays, a trypsin inhibition assay and a haemolysis assay, were conducted and the results showed that Lividin-AW exhibited weak antimicrobial activity against Candida albicans (MIC=128µM), Staphylococcus aureus (MIC=512 µM) and Escherichia coli (MIC=512 µM), with negligible haemolytic activity, but neither trypsin inhibition activity nor anticancer activity were detected.","abstract_has_math":false,"creators":["Lu, Kelei"],"institution":"Queen's University Belfast","degree_name":"Master of Philosophy","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Shaw, Christopher","Chen, Tianbao","Zhou, Mei","Wang, Lei","Ma, Chengbang"],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-8-25","date_published":"2017-8-25","updated_at":"2026-07-24T03:54:46Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/629396ea-5c91-4106-8860-aca9a090e1ac"],"render_values":[{"text":"oai:pure.qub.ac.uk/portal:studenttheses/629396ea-5c91-4106-8860-aca9a090e1ac","href":null,"code":true}]}]},"links":{"outbound_url":"https://pure.qub.ac.uk/en/studentTheses/629396ea-5c91-4106-8860-aca9a090e1ac","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Shaw, Christopher","Chen, Tianbao","Zhou, Mei","Wang, Lei","Ma, Chengbang"]},{"key":"dc:creator","label":"Author","values":["Lu, Kelei"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2017-8-25"]},{"key":"dc:date.issued","label":"Date","values":["2017-8-25"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Pharmacy"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Queen's University Belfast"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://pure.qub.ac.uk/en/studentTheses/629396ea-5c91-4106-8860-aca9a090e1ac"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Master of Philosophy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights.embargodate","label":"Dc Rights Embargodate","values":["2022-09-01"]},{"key":"dc:rights.embargoreason","label":"Dc Rights Embargoreason","values":["/dk/atira/pure/core/document/studentthesisembargoreason/nationalsecurity"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/629396ea-5c91-4106-8860-aca9a090e1ac","https://pure.qub.ac.uk/en/studentTheses/629396ea-5c91-4106-8860-aca9a090e1ac"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://pure.qub.ac.uk/files/134674834/2017MPhil_Thesis_Kelei_Lu.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Peptides derived from frog skin secretion have great potential as drug leads as they exhibit many bioactive functions. In this study, the precursor peptide of Lividin-AW was identified from the skin secretion of the Chinese torrent frog (Amolops wuyiensis) by using ‘shotgun’ cloning and its encoded mature peptide, sequenced as AVPLIYNRPGVYVTKRPK-NH2, was synthesised by Fmoc-solid phase peptide synthesis. The structure of the synthetic peptide was confirmed by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry and purified by reversed-phase high performance liquid chromatography (RP-HPLC). To determine the bioactive function of Lividin-AW, a series of bioactivity assays, including antimicrobial assays, cell viability assays, a trypsin inhibition assay and a haemolysis assay, were conducted and the results showed that Lividin-AW exhibited weak antimicrobial activity against Candida albicans (MIC=128µM), Staphylococcus aureus (MIC=512 µM) and Escherichia coli (MIC=512 µM), with negligible haemolytic activity, but neither trypsin inhibition activity nor anticancer activity were detected."]},{"key":"dc:title","label":"Title","values":["A bioactive peptide from the skin of the Chinese Torrent Frog, <i>Amolops wuyiensis</i>"]}]}],"canonical_facts":{"dc:contributor.advisor":["Shaw, Christopher","Chen, Tianbao","Zhou, Mei","Wang, Lei","Ma, Chengbang"],"dc:creator":["Lu, Kelei"],"dc:date":["2017-8-25"],"dc:date.issued":["2017-8-25"],"dc:description.abstract":["Peptides derived from frog skin secretion have great potential as drug leads as they exhibit many bioactive functions. 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