{"id":{"repo_id":"qu-belfast","oai_identifier":"oai:pure.qub.ac.uk/portal:studenttheses/5533516c-3608-4c1e-8c4e-4c16e3c79c0e"},"canonical_url":"https://search.dev.ndltd.org/etd/qu-belfast/oai:pure.qub.ac.uk/portal:studenttheses/5533516c-3608-4c1e-8c4e-4c16e3c79c0e","repository":{"repo_id":"qu-belfast","name":"Queen's University Belfast","base_url":"https://pureadmin.qub.ac.uk/ws/oai"},"display":{"title":"Identification and functional study of the bioactive peptide QUB-2417 from the skin secretion of the tree frog, Phyllomedusa bicolor","abstract":"With the rising of antibiotic resistance, it is urgent to search for new antimicrobials, and many scholars have focused on AMPs. Generally, it is difficult for pathogenic microorganisms to develop resistance to AMPs because of their action mechanism. In this thesis, an AMP was identified from the skin secretion of Phyllomedusa bicolor, a tree frog from the Amazon rainforest, and was named QUB-2417. The peptide sequence was acquired by molecular cloning through constructing a cDNA library with isolated mRNA from skin secretion. Then the peptide was chemically synthesised by Solid Phase Peptide Synthesis, purified by RP-HPLC, and then analysed by MALDI-TOF MS to identify the peptide. In the antimicrobial assay, QUB-2417 exhibited potent and broad-spectrum antibacterial activity. Its MICs against S. aureus, E. coli and C. albicans were 4, 16 and 16 μM, respectively. In the MTT assay, QUB-2417 exhibited bioactivity against lung cancer cell line NCI-H838 at the highest test concentration (10-4 M) and the value of IC50 was 35.3 µM. In the haemolysis bioassay, QUB-2417 exhibited about 30% haemolytic effect at the highest test concentration (128 μM) and the value of HC50 was 690 µM. Hence, the peptide deserves serious attention as a potential therapeutic due to its potent relevant bioactivity and is expected to be optimised as a good drug candidate.<br/><i><br/>Thesis embargoed until 31 December 2026</i>.","abstract_html":"With the rising of antibiotic resistance, it is urgent to search for new antimicrobials, and many scholars have focused on AMPs. Generally, it is difficult for pathogenic microorganisms to develop resistance to AMPs because of their action mechanism. In this thesis, an AMP was identified from the skin secretion of Phyllomedusa bicolor, a tree frog from the Amazon rainforest, and was named QUB-2417. The peptide sequence was acquired by molecular cloning through constructing a cDNA library with isolated mRNA from skin secretion. Then the peptide was chemically synthesised by Solid Phase Peptide Synthesis, purified by RP-HPLC, and then analysed by MALDI-TOF MS to identify the peptide. In the antimicrobial assay, QUB-2417 exhibited potent and broad-spectrum antibacterial activity. Its MICs against S. aureus, E. coli and C. albicans were 4, 16 and 16 μM, respectively. In the MTT assay, QUB-2417 exhibited bioactivity against lung cancer cell line NCI-H838 at the highest test concentration (10-4 M) and the value of IC50 was 35.3 µM. In the haemolysis bioassay, QUB-2417 exhibited about 30% haemolytic effect at the highest test concentration (128 μM) and the value of HC50 was 690 µM. Hence, the peptide deserves serious attention as a potential therapeutic due to its potent relevant bioactivity and is expected to be optimised as a good drug candidate.&lt;br/&gt;&lt;i&gt;&lt;br/&gt;Thesis embargoed until 31 December 2026&lt;/i&gt;.","abstract_has_math":false,"creators":["Ren, Haomiao"],"institution":"Queen's University Belfast","degree_name":"Master of Philosophy","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Wang, Lei","Zhou, Mei","Chen, Tianbao"],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-12","date_published":"2021-12","updated_at":"2026-07-24T03:56:04Z","subjects":["Antimicrobial peptides","molecular cloning","bioactivity evaluation","antimicrobial activity","non-small lung cancer cells","cytotoxicity"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/5533516c-3608-4c1e-8c4e-4c16e3c79c0e"],"render_values":[{"text":"oai:pure.qub.ac.uk/portal:studenttheses/5533516c-3608-4c1e-8c4e-4c16e3c79c0e","href":null,"code":true}]}]},"links":{"outbound_url":"https://pure.qub.ac.uk/en/studentTheses/5533516c-3608-4c1e-8c4e-4c16e3c79c0e","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Wang, Lei","Zhou, Mei","Chen, Tianbao"]},{"key":"dc:contributor.sponsor","label":"Sponsor","values":["Queen's University & China Scholarship Council"]},{"key":"dc:creator","label":"Author","values":["Ren, Haomiao"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2021-12"]},{"key":"dc:date.issued","label":"Date","values":["2021-12"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Pharmacy"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Queen's University Belfast"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://pure.qub.ac.uk/en/studentTheses/5533516c-3608-4c1e-8c4e-4c16e3c79c0e"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Master of Philosophy"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Antimicrobial peptides","molecular cloning","bioactivity evaluation","antimicrobial activity","non-small lung cancer cells","cytotoxicity"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights.embargodate","label":"Dc Rights Embargodate","values":["2026-12-31"]},{"key":"dc:rights.embargoreason","label":"Dc Rights Embargoreason","values":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/5533516c-3608-4c1e-8c4e-4c16e3c79c0e","https://pure.qub.ac.uk/en/studentTheses/5533516c-3608-4c1e-8c4e-4c16e3c79c0e"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["With the rising of antibiotic resistance, it is urgent to search for new antimicrobials, and many scholars have focused on AMPs. Generally, it is difficult for pathogenic microorganisms to develop resistance to AMPs because of their action mechanism. In this thesis, an AMP was identified from the skin secretion of Phyllomedusa bicolor, a tree frog from the Amazon rainforest, and was named QUB-2417. The peptide sequence was acquired by molecular cloning through constructing a cDNA library with isolated mRNA from skin secretion. Then the peptide was chemically synthesised by Solid Phase Peptide Synthesis, purified by RP-HPLC, and then analysed by MALDI-TOF MS to identify the peptide. In the antimicrobial assay, QUB-2417 exhibited potent and broad-spectrum antibacterial activity. Its MICs against S. aureus, E. coli and C. albicans were 4, 16 and 16 μM, respectively. In the MTT assay, QUB-2417 exhibited bioactivity against lung cancer cell line NCI-H838 at the highest test concentration (10-4 M) and the value of IC50 was 35.3 µM. In the haemolysis bioassay, QUB-2417 exhibited about 30% haemolytic effect at the highest test concentration (128 μM) and the value of HC50 was 690 µM. Hence, the peptide deserves serious attention as a potential therapeutic due to its potent relevant bioactivity and is expected to be optimised as a good drug candidate.<br/><i><br/>Thesis embargoed until 31 December 2026</i>."]},{"key":"dc:title","label":"Title","values":["Identification and functional study of the bioactive peptide QUB-2417 from the skin secretion of the tree frog, Phyllomedusa bicolor"]}]}],"canonical_facts":{"dc:contributor.advisor":["Wang, Lei","Zhou, Mei","Chen, Tianbao"],"dc:contributor.sponsor":["Queen's University & China Scholarship Council"],"dc:creator":["Ren, Haomiao"],"dc:date":["2021-12"],"dc:date.issued":["2021-12"],"dc:description.abstract":["With the rising of antibiotic resistance, it is urgent to search for new antimicrobials, and many scholars have focused on AMPs. Generally, it is difficult for pathogenic microorganisms to develop resistance to AMPs because of their action mechanism. In this thesis, an AMP was identified from the skin secretion of Phyllomedusa bicolor, a tree frog from the Amazon rainforest, and was named QUB-2417. The peptide sequence was acquired by molecular cloning through constructing a cDNA library with isolated mRNA from skin secretion. Then the peptide was chemically synthesised by Solid Phase Peptide Synthesis, purified by RP-HPLC, and then analysed by MALDI-TOF MS to identify the peptide. In the antimicrobial assay, QUB-2417 exhibited potent and broad-spectrum antibacterial activity. Its MICs against S. aureus, E. coli and C. albicans were 4, 16 and 16 μM, respectively. In the MTT assay, QUB-2417 exhibited bioactivity against lung cancer cell line NCI-H838 at the highest test concentration (10-4 M) and the value of IC50 was 35.3 µM. In the haemolysis bioassay, QUB-2417 exhibited about 30% haemolytic effect at the highest test concentration (128 μM) and the value of HC50 was 690 µM. Hence, the peptide deserves serious attention as a potential therapeutic due to its potent relevant bioactivity and is expected to be optimised as a good drug candidate.<br/><i><br/>Thesis embargoed until 31 December 2026</i>."],"dc:identifier":["oai:pure.qub.ac.uk/portal:studenttheses/5533516c-3608-4c1e-8c4e-4c16e3c79c0e","https://pure.qub.ac.uk/en/studentTheses/5533516c-3608-4c1e-8c4e-4c16e3c79c0e"],"dc:language":["eng"],"dc:publisher.department":["School of Pharmacy"],"dc:publisher.institution":["Queen's University Belfast"],"dc:relation.isreferencedby":["https://pure.qub.ac.uk/en/studentTheses/5533516c-3608-4c1e-8c4e-4c16e3c79c0e"],"dc:rights.embargodate":["2026-12-31"],"dc:rights.embargoreason":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"],"dc:subject":["Antimicrobial peptides","molecular cloning","bioactivity evaluation","antimicrobial activity","non-small lung cancer cells","cytotoxicity"],"dc:title":["Identification and functional study of the bioactive peptide QUB-2417 from the skin secretion of the tree frog, Phyllomedusa bicolor"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["Masters Thesis"],"dc:type.qualificationname":["Master of Philosophy"]},"updated_at":"2026-07-24T03:56:04Z"}