{"id":{"repo_id":"qu-belfast","oai_identifier":"oai:pure.qub.ac.uk/portal:studenttheses/40f944e4-2e2e-4e02-86b3-fd357af94141"},"canonical_url":"https://search.dev.ndltd.org/etd/qu-belfast/oai:pure.qub.ac.uk/portal:studenttheses/40f944e4-2e2e-4e02-86b3-fd357af94141","repository":{"repo_id":"qu-belfast","name":"Queen's University Belfast","base_url":"https://pureadmin.qub.ac.uk/ws/oai"},"display":{"title":"Identification and functional study of a bioactive peptide QUB-1962 from the skin secretion of the yellow-bellied toad (Bombina variegata)","abstract":"Because of the continuous emergence of drug-resistant strains, more and more antibiotics have lost their due role. The antimicrobial peptide has become the most promising substance in the next class of antimicrobial agents. QUB-1962 is a cationic antimicrobial peptide isolated from the skin secretions of Bombina variegata. Using the \"shotgun\" cloning technique, a novel full-length peptide precursor of the bombinin peptide was cloned from the cDNA library of skin secretion from Bombina variegata. The speculative peptide was identified as QUB-1962 (IIGPVLGMVGNALGGLLKKI-NH2). And the crude peptide of QUB-1962 was obtained by solid-phase synthesis. Then, QUB-1962 was identified in HPLC fractions based on their molecular masses determined by MALDI-TOF MS. In addition, QUB-1962 exhibited a broad-spectrum antimicrobial effect against Gram-positive bacteria Staphylococcus aureus (S. aureus), Gram-negative bacteria Escherichia coli (E. coli), and fungus Candida albicans (C. albicans) and has relatively weak haemolytic activity (HC50 is 691.6 μl). The anti-cancer activity assay showed that the peptide had a significant anti-cancer effect (IC50: 3.471×10-5 M) on the tested human cancer cell glioblastoma U251 MG. According to the data of these biological activities, QUB-1962 has a wide range of biological activities, including antimicrobial and anticancer activities. At the same time, it could be a good candidate for the development of new anti-cancer and antimicrobial infection drugs.","abstract_html":"Because of the continuous emergence of drug-resistant strains, more and more antibiotics have lost their due role. The antimicrobial peptide has become the most promising substance in the next class of antimicrobial agents. QUB-1962 is a cationic antimicrobial peptide isolated from the skin secretions of Bombina variegata. Using the "shotgun" cloning technique, a novel full-length peptide precursor of the bombinin peptide was cloned from the cDNA library of skin secretion from Bombina variegata. The speculative peptide was identified as QUB-1962 (IIGPVLGMVGNALGGLLKKI-NH2). And the crude peptide of QUB-1962 was obtained by solid-phase synthesis. Then, QUB-1962 was identified in HPLC fractions based on their molecular masses determined by MALDI-TOF MS. In addition, QUB-1962 exhibited a broad-spectrum antimicrobial effect against Gram-positive bacteria Staphylococcus aureus (S. aureus), Gram-negative bacteria Escherichia coli (E. coli), and fungus Candida albicans (C. albicans) and has relatively weak haemolytic activity (HC50 is 691.6 μl). The anti-cancer activity assay showed that the peptide had a significant anti-cancer effect (IC50: 3.471×10-5 M) on the tested human cancer cell glioblastoma U251 MG. According to the data of these biological activities, QUB-1962 has a wide range of biological activities, including antimicrobial and anticancer activities. At the same time, it could be a good candidate for the development of new anti-cancer and antimicrobial infection drugs.","abstract_has_math":false,"creators":["Meng, Fansen"],"institution":"Queen's University Belfast","degree_name":"Master of Philosophy","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Zhou, Mei","Wang, Lei","Chen, Tianbao"],"committee_chairs":[],"committee_members":[],"year":2020,"date_issued":"2020-12","date_published":"2020-12","updated_at":"2026-07-24T03:55:31Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/40f944e4-2e2e-4e02-86b3-fd357af94141"],"render_values":[{"text":"oai:pure.qub.ac.uk/portal:studenttheses/40f944e4-2e2e-4e02-86b3-fd357af94141","href":null,"code":true}]}]},"links":{"outbound_url":"https://pure.qub.ac.uk/en/studentTheses/40f944e4-2e2e-4e02-86b3-fd357af94141","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Zhou, Mei","Wang, Lei","Chen, Tianbao"]},{"key":"dc:creator","label":"Author","values":["Meng, Fansen"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2020-12"]},{"key":"dc:date.issued","label":"Date","values":["2020-12"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Pharmacy"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Queen's University Belfast"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://pure.qub.ac.uk/en/studentTheses/40f944e4-2e2e-4e02-86b3-fd357af94141"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Master of Philosophy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights.embargodate","label":"Dc Rights Embargodate","values":["2025-12-31"]},{"key":"dc:rights.embargoreason","label":"Dc Rights Embargoreason","values":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/40f944e4-2e2e-4e02-86b3-fd357af94141","https://pure.qub.ac.uk/en/studentTheses/40f944e4-2e2e-4e02-86b3-fd357af94141"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://pure.qub.ac.uk/files/220810070/Identification_and_functional_study_of_a_bioactive_peptide_QUB_1962_from_the_skin_secretion_of_The_Yellow_Bellied_Toad_Bombina_variegata_.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Because of the continuous emergence of drug-resistant strains, more and more antibiotics have lost their due role. The antimicrobial peptide has become the most promising substance in the next class of antimicrobial agents. QUB-1962 is a cationic antimicrobial peptide isolated from the skin secretions of Bombina variegata. Using the \"shotgun\" cloning technique, a novel full-length peptide precursor of the bombinin peptide was cloned from the cDNA library of skin secretion from Bombina variegata. The speculative peptide was identified as QUB-1962 (IIGPVLGMVGNALGGLLKKI-NH2). And the crude peptide of QUB-1962 was obtained by solid-phase synthesis. Then, QUB-1962 was identified in HPLC fractions based on their molecular masses determined by MALDI-TOF MS. In addition, QUB-1962 exhibited a broad-spectrum antimicrobial effect against Gram-positive bacteria Staphylococcus aureus (S. aureus), Gram-negative bacteria Escherichia coli (E. coli), and fungus Candida albicans (C. albicans) and has relatively weak haemolytic activity (HC50 is 691.6 μl). The anti-cancer activity assay showed that the peptide had a significant anti-cancer effect (IC50: 3.471×10-5 M) on the tested human cancer cell glioblastoma U251 MG. According to the data of these biological activities, QUB-1962 has a wide range of biological activities, including antimicrobial and anticancer activities. At the same time, it could be a good candidate for the development of new anti-cancer and antimicrobial infection drugs."]},{"key":"dc:title","label":"Title","values":["Identification and functional study of a bioactive peptide QUB-1962 from the skin secretion of the yellow-bellied toad (Bombina variegata)"]}]}],"canonical_facts":{"dc:contributor.advisor":["Zhou, Mei","Wang, Lei","Chen, Tianbao"],"dc:creator":["Meng, Fansen"],"dc:date":["2020-12"],"dc:date.issued":["2020-12"],"dc:description.abstract":["Because of the continuous emergence of drug-resistant strains, more and more antibiotics have lost their due role. The antimicrobial peptide has become the most promising substance in the next class of antimicrobial agents. QUB-1962 is a cationic antimicrobial peptide isolated from the skin secretions of Bombina variegata. Using the \"shotgun\" cloning technique, a novel full-length peptide precursor of the bombinin peptide was cloned from the cDNA library of skin secretion from Bombina variegata. The speculative peptide was identified as QUB-1962 (IIGPVLGMVGNALGGLLKKI-NH2). And the crude peptide of QUB-1962 was obtained by solid-phase synthesis. Then, QUB-1962 was identified in HPLC fractions based on their molecular masses determined by MALDI-TOF MS. In addition, QUB-1962 exhibited a broad-spectrum antimicrobial effect against Gram-positive bacteria Staphylococcus aureus (S. aureus), Gram-negative bacteria Escherichia coli (E. coli), and fungus Candida albicans (C. albicans) and has relatively weak haemolytic activity (HC50 is 691.6 μl). The anti-cancer activity assay showed that the peptide had a significant anti-cancer effect (IC50: 3.471×10-5 M) on the tested human cancer cell glioblastoma U251 MG. According to the data of these biological activities, QUB-1962 has a wide range of biological activities, including antimicrobial and anticancer activities. 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