{"id":{"repo_id":"qu-belfast","oai_identifier":"oai:pure.qub.ac.uk/portal:studenttheses/2a6aa10f-755e-4e1c-b1bb-1f44481a6825"},"canonical_url":"https://search.dev.ndltd.org/etd/qu-belfast/oai:pure.qub.ac.uk/portal:studenttheses/2a6aa10f-755e-4e1c-b1bb-1f44481a6825","repository":{"repo_id":"qu-belfast","name":"Queen's University Belfast","base_url":"https://pureadmin.qub.ac.uk/ws/oai"},"display":{"title":"Identification and functional study of a novel bioactive peptide QUB-1761 from the venom of the Brazilian yellow scorpion, Tityus serrulatus","abstract":"Scorpion venom contains a variety of peptides exerting broad bioactivities. In this thesis, QUB-1761, a non-disulphide bridged peptide (NDBP), was discovered in the venom of the Brazilian yellow scorpion, Tityus serrulatus (T. serrulatus). The peptide was identified through molecular cloning by using a sense primer. The synthetic peptide was obtained by solid phase peptide synthesis (SPPS), followed by purification and confirmation through reversed-phase high performance liquid chromatography (RP-HPLC) and matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS). In broth microdilution assays, QUB-1761 had strong inhibitory effects on both Gram-positive and Gram-negative bacteria (MIC=8 µM for Staphylococcus aureus, MIC=32 µM for Escherichia coli). However, it was of low potency against yeast (MIC=128 µM for Candida albicans). In MTT anticancer cell proliferation and trypan blue exclusion assays, QUB-1761 beyond the concentration of 40 µM can completely kill the human lung cancer cells (NCI-H838). In terms of the haemolytic assays, QUB-1761 showed about 30% haemolysis activity at 64 µM and more than 50% at 128 µM. Nonetheless, QUB-1761 has the potential to be used in the design of drugs to treat bacterial infections or cancer if optimised.<br/><br/><i>Thesis embargoed until 31 December 2026</i>.","abstract_html":"Scorpion venom contains a variety of peptides exerting broad bioactivities. In this thesis, QUB-1761, a non-disulphide bridged peptide (NDBP), was discovered in the venom of the Brazilian yellow scorpion, Tityus serrulatus (T. serrulatus). The peptide was identified through molecular cloning by using a sense primer. The synthetic peptide was obtained by solid phase peptide synthesis (SPPS), followed by purification and confirmation through reversed-phase high performance liquid chromatography (RP-HPLC) and matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS). In broth microdilution assays, QUB-1761 had strong inhibitory effects on both Gram-positive and Gram-negative bacteria (MIC=8 µM for Staphylococcus aureus, MIC=32 µM for Escherichia coli). However, it was of low potency against yeast (MIC=128 µM for Candida albicans). In MTT anticancer cell proliferation and trypan blue exclusion assays, QUB-1761 beyond the concentration of 40 µM can completely kill the human lung cancer cells (NCI-H838). In terms of the haemolytic assays, QUB-1761 showed about 30% haemolysis activity at 64 µM and more than 50% at 128 µM. Nonetheless, QUB-1761 has the potential to be used in the design of drugs to treat bacterial infections or cancer if optimised.&lt;br/&gt;&lt;br/&gt;&lt;i&gt;Thesis embargoed until 31 December 2026&lt;/i&gt;.","abstract_has_math":false,"creators":["Wang, Xinyue"],"institution":"Queen's University Belfast","degree_name":"Master of Philosophy","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Zhou, Mei","Chen, Tianbao","Wang, Lei"],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-12","date_published":"2021-12","updated_at":"2026-07-24T03:56:04Z","subjects":["Scorpion venom","non-disulphide bridged peptide","Gram-positive and Gram-negative bacteria","human lung cancer","haemolysis activity"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/2a6aa10f-755e-4e1c-b1bb-1f44481a6825"],"render_values":[{"text":"oai:pure.qub.ac.uk/portal:studenttheses/2a6aa10f-755e-4e1c-b1bb-1f44481a6825","href":null,"code":true}]}]},"links":{"outbound_url":"https://pure.qub.ac.uk/en/studentTheses/2a6aa10f-755e-4e1c-b1bb-1f44481a6825","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Zhou, Mei","Chen, Tianbao","Wang, Lei"]},{"key":"dc:contributor.sponsor","label":"Sponsor","values":["Queen's University & China Scholarship Council"]},{"key":"dc:creator","label":"Author","values":["Wang, Xinyue"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2021-12"]},{"key":"dc:date.issued","label":"Date","values":["2021-12"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Pharmacy"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Queen's University Belfast"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://pure.qub.ac.uk/en/studentTheses/2a6aa10f-755e-4e1c-b1bb-1f44481a6825"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Master of Philosophy"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Scorpion venom","non-disulphide bridged peptide","Gram-positive and Gram-negative bacteria","human lung cancer","haemolysis activity"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights.embargodate","label":"Dc Rights Embargodate","values":["2026-12-31"]},{"key":"dc:rights.embargoreason","label":"Dc Rights Embargoreason","values":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/2a6aa10f-755e-4e1c-b1bb-1f44481a6825","https://pure.qub.ac.uk/en/studentTheses/2a6aa10f-755e-4e1c-b1bb-1f44481a6825"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Scorpion venom contains a variety of peptides exerting broad bioactivities. In this thesis, QUB-1761, a non-disulphide bridged peptide (NDBP), was discovered in the venom of the Brazilian yellow scorpion, Tityus serrulatus (T. serrulatus). The peptide was identified through molecular cloning by using a sense primer. The synthetic peptide was obtained by solid phase peptide synthesis (SPPS), followed by purification and confirmation through reversed-phase high performance liquid chromatography (RP-HPLC) and matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS). In broth microdilution assays, QUB-1761 had strong inhibitory effects on both Gram-positive and Gram-negative bacteria (MIC=8 µM for Staphylococcus aureus, MIC=32 µM for Escherichia coli). However, it was of low potency against yeast (MIC=128 µM for Candida albicans). In MTT anticancer cell proliferation and trypan blue exclusion assays, QUB-1761 beyond the concentration of 40 µM can completely kill the human lung cancer cells (NCI-H838). In terms of the haemolytic assays, QUB-1761 showed about 30% haemolysis activity at 64 µM and more than 50% at 128 µM. Nonetheless, QUB-1761 has the potential to be used in the design of drugs to treat bacterial infections or cancer if optimised.<br/><br/><i>Thesis embargoed until 31 December 2026</i>."]},{"key":"dc:title","label":"Title","values":["Identification and functional study of a novel bioactive peptide QUB-1761 from the venom of the Brazilian yellow scorpion, Tityus serrulatus"]}]}],"canonical_facts":{"dc:contributor.advisor":["Zhou, Mei","Chen, Tianbao","Wang, Lei"],"dc:contributor.sponsor":["Queen's University & China Scholarship Council"],"dc:creator":["Wang, Xinyue"],"dc:date":["2021-12"],"dc:date.issued":["2021-12"],"dc:description.abstract":["Scorpion venom contains a variety of peptides exerting broad bioactivities. In this thesis, QUB-1761, a non-disulphide bridged peptide (NDBP), was discovered in the venom of the Brazilian yellow scorpion, Tityus serrulatus (T. serrulatus). The peptide was identified through molecular cloning by using a sense primer. The synthetic peptide was obtained by solid phase peptide synthesis (SPPS), followed by purification and confirmation through reversed-phase high performance liquid chromatography (RP-HPLC) and matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS). In broth microdilution assays, QUB-1761 had strong inhibitory effects on both Gram-positive and Gram-negative bacteria (MIC=8 µM for Staphylococcus aureus, MIC=32 µM for Escherichia coli). However, it was of low potency against yeast (MIC=128 µM for Candida albicans). In MTT anticancer cell proliferation and trypan blue exclusion assays, QUB-1761 beyond the concentration of 40 µM can completely kill the human lung cancer cells (NCI-H838). In terms of the haemolytic assays, QUB-1761 showed about 30% haemolysis activity at 64 µM and more than 50% at 128 µM. Nonetheless, QUB-1761 has the potential to be used in the design of drugs to treat bacterial infections or cancer if optimised.<br/><br/><i>Thesis embargoed until 31 December 2026</i>."],"dc:identifier":["oai:pure.qub.ac.uk/portal:studenttheses/2a6aa10f-755e-4e1c-b1bb-1f44481a6825","https://pure.qub.ac.uk/en/studentTheses/2a6aa10f-755e-4e1c-b1bb-1f44481a6825"],"dc:language":["eng"],"dc:publisher.department":["School of Pharmacy"],"dc:publisher.institution":["Queen's University Belfast"],"dc:relation.isreferencedby":["https://pure.qub.ac.uk/en/studentTheses/2a6aa10f-755e-4e1c-b1bb-1f44481a6825"],"dc:rights.embargodate":["2026-12-31"],"dc:rights.embargoreason":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"],"dc:subject":["Scorpion venom","non-disulphide bridged peptide","Gram-positive and Gram-negative bacteria","human lung cancer","haemolysis activity"],"dc:title":["Identification and functional study of a novel bioactive peptide QUB-1761 from the venom of the Brazilian yellow scorpion, Tityus serrulatus"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["Masters Thesis"],"dc:type.qualificationname":["Master of Philosophy"]},"updated_at":"2026-07-24T03:56:04Z"}