{"id":{"repo_id":"qu-belfast","oai_identifier":"oai:pure.qub.ac.uk/portal:studenttheses/1eefd22f-a0f3-470e-ae50-77aaeb115b6a"},"canonical_url":"https://search.dev.ndltd.org/etd/qu-belfast/oai:pure.qub.ac.uk/portal:studenttheses/1eefd22f-a0f3-470e-ae50-77aaeb115b6a","repository":{"repo_id":"qu-belfast","name":"Queen's University Belfast","base_url":"https://pureadmin.qub.ac.uk/ws/oai"},"display":{"title":"A bioactive peptide QUB2870 from the defensive skin secretion of the Heilongjiang brown frog (Rana amurensis)","abstract":"The skin secretions of amphibians consist of a variety of peptides with different bioactivities which have important roles in supporting amphibians to survive in complex environments and against predators. Many of these peptides possess anticancer, antiviral, trypsin inhibitory and antimicrobial properties, and therefore their potential therapeutic value has been focused on by increasing numbers of researchers.<br/><br/>The main objectives of this study were to isolate, identify, chemically-synthesise and clone the precursor-encoding cDNA of a bioactive peptide from the skin secretion of the Heilongjiang brown frog (Rana amurensis). A “shotgun” cloning technique was employed to select the precursor sequence and a cDNA library was established for amplification of the target DNA portion. The peptide was isolated and identified using molecular cloning, online BLAST analysis, and MALDI-TOF mass spectrometry.<br/><br/>The mature peptide, deduced from cDNA, was named QUB-2870, (SLLGLLKGAGKTLLSAGLNKIACKLTGKC-COOH), and consisted of 29 amino acid residues. It was synthesised by the method of solid phase peptide synthesis (SPPS) and further purified by rp-HPLC. Subsequently, QUB-2870 was found to have powerful activity against the Gram-positive bacterium, S. aureus, the Gram-negative bacterium E. coli and the yeast, C. albicans. Moreover, QUB-2870 was found to have significant growth inhibitory effects on PC-3, H157, MB435S, and U251 cancer cell lines.","abstract_html":"The skin secretions of amphibians consist of a variety of peptides with different bioactivities which have important roles in supporting amphibians to survive in complex environments and against predators. Many of these peptides possess anticancer, antiviral, trypsin inhibitory and antimicrobial properties, and therefore their potential therapeutic value has been focused on by increasing numbers of researchers.&lt;br/&gt;&lt;br/&gt;The main objectives of this study were to isolate, identify, chemically-synthesise and clone the precursor-encoding cDNA of a bioactive peptide from the skin secretion of the Heilongjiang brown frog (Rana amurensis). A “shotgun” cloning technique was employed to select the precursor sequence and a cDNA library was established for amplification of the target DNA portion. The peptide was isolated and identified using molecular cloning, online BLAST analysis, and MALDI-TOF mass spectrometry.&lt;br/&gt;&lt;br/&gt;The mature peptide, deduced from cDNA, was named QUB-2870, (SLLGLLKGAGKTLLSAGLNKIACKLTGKC-COOH), and consisted of 29 amino acid residues. It was synthesised by the method of solid phase peptide synthesis (SPPS) and further purified by rp-HPLC. Subsequently, QUB-2870 was found to have powerful activity against the Gram-positive bacterium, S. aureus, the Gram-negative bacterium E. coli and the yeast, C. albicans. Moreover, QUB-2870 was found to have significant growth inhibitory effects on PC-3, H157, MB435S, and U251 cancer cell lines.","abstract_has_math":false,"creators":["Wang, Xiaoqing"],"institution":"Queen's University Belfast","degree_name":"Master of Philosophy","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Wang, Lei","Zhou, Mei","Chen, Tianbao"],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-8-29","date_published":"2017-8-29","updated_at":"2026-07-24T03:54:46Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/1eefd22f-a0f3-470e-ae50-77aaeb115b6a"],"render_values":[{"text":"oai:pure.qub.ac.uk/portal:studenttheses/1eefd22f-a0f3-470e-ae50-77aaeb115b6a","href":null,"code":true}]}]},"links":{"outbound_url":"https://pure.qub.ac.uk/en/studentTheses/1eefd22f-a0f3-470e-ae50-77aaeb115b6a","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Wang, Lei","Zhou, Mei","Chen, Tianbao"]},{"key":"dc:creator","label":"Author","values":["Wang, Xiaoqing"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2017-8-29"]},{"key":"dc:date.issued","label":"Date","values":["2017-8-29"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Pharmacy"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Queen's University Belfast"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://pure.qub.ac.uk/en/studentTheses/1eefd22f-a0f3-470e-ae50-77aaeb115b6a"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Master of Philosophy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights.embargodate","label":"Dc Rights Embargodate","values":["2022-10-01"]},{"key":"dc:rights.embargoreason","label":"Dc Rights Embargoreason","values":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/1eefd22f-a0f3-470e-ae50-77aaeb115b6a","https://pure.qub.ac.uk/en/studentTheses/1eefd22f-a0f3-470e-ae50-77aaeb115b6a"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://pure.qub.ac.uk/files/135177082/Wang_Xiaoqing_MPhil_Thesis.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The skin secretions of amphibians consist of a variety of peptides with different bioactivities which have important roles in supporting amphibians to survive in complex environments and against predators. Many of these peptides possess anticancer, antiviral, trypsin inhibitory and antimicrobial properties, and therefore their potential therapeutic value has been focused on by increasing numbers of researchers.<br/><br/>The main objectives of this study were to isolate, identify, chemically-synthesise and clone the precursor-encoding cDNA of a bioactive peptide from the skin secretion of the Heilongjiang brown frog (Rana amurensis). A “shotgun” cloning technique was employed to select the precursor sequence and a cDNA library was established for amplification of the target DNA portion. The peptide was isolated and identified using molecular cloning, online BLAST analysis, and MALDI-TOF mass spectrometry.<br/><br/>The mature peptide, deduced from cDNA, was named QUB-2870, (SLLGLLKGAGKTLLSAGLNKIACKLTGKC-COOH), and consisted of 29 amino acid residues. It was synthesised by the method of solid phase peptide synthesis (SPPS) and further purified by rp-HPLC. Subsequently, QUB-2870 was found to have powerful activity against the Gram-positive bacterium, S. aureus, the Gram-negative bacterium E. coli and the yeast, C. albicans. Moreover, QUB-2870 was found to have significant growth inhibitory effects on PC-3, H157, MB435S, and U251 cancer cell lines."]},{"key":"dc:title","label":"Title","values":["A bioactive peptide QUB2870 from the defensive skin secretion of the Heilongjiang brown frog (Rana amurensis)"]}]}],"canonical_facts":{"dc:contributor.advisor":["Wang, Lei","Zhou, Mei","Chen, Tianbao"],"dc:creator":["Wang, Xiaoqing"],"dc:date":["2017-8-29"],"dc:date.issued":["2017-8-29"],"dc:description.abstract":["The skin secretions of amphibians consist of a variety of peptides with different bioactivities which have important roles in supporting amphibians to survive in complex environments and against predators. Many of these peptides possess anticancer, antiviral, trypsin inhibitory and antimicrobial properties, and therefore their potential therapeutic value has been focused on by increasing numbers of researchers.<br/><br/>The main objectives of this study were to isolate, identify, chemically-synthesise and clone the precursor-encoding cDNA of a bioactive peptide from the skin secretion of the Heilongjiang brown frog (Rana amurensis). A “shotgun” cloning technique was employed to select the precursor sequence and a cDNA library was established for amplification of the target DNA portion. The peptide was isolated and identified using molecular cloning, online BLAST analysis, and MALDI-TOF mass spectrometry.<br/><br/>The mature peptide, deduced from cDNA, was named QUB-2870, (SLLGLLKGAGKTLLSAGLNKIACKLTGKC-COOH), and consisted of 29 amino acid residues. It was synthesised by the method of solid phase peptide synthesis (SPPS) and further purified by rp-HPLC. Subsequently, QUB-2870 was found to have powerful activity against the Gram-positive bacterium, S. aureus, the Gram-negative bacterium E. coli and the yeast, C. albicans. 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