{"id":{"repo_id":"qu-belfast","oai_identifier":"oai:pure.qub.ac.uk/portal:studenttheses/1859873e-163d-4a3f-b989-ef1b1986b179"},"canonical_url":"https://search.dev.ndltd.org/etd/qu-belfast/oai:pure.qub.ac.uk/portal:studenttheses/1859873e-163d-4a3f-b989-ef1b1986b179","repository":{"repo_id":"qu-belfast","name":"Queen's University Belfast","base_url":"https://pureadmin.qub.ac.uk/ws/oai"},"display":{"title":"Bioactive evaluations of novel peptide QUB-2552 from the skin secretion of the Chinese Large Odorous frog, Rana (Odorrana) livida","abstract":"Amphibian skin secretions are rich resources of bioactive peptides, some of which could be developed as drug candidates. Among these natural peptides, antimicrobial peptides have attracted great attention given that they might help to deal with the problem of antibiotic resistance. In this study, the precursor-encoding cDNA sequence of a novel bioactive peptide QUB-2552 was identified in the skin secretion of Chinese Large Odorous frog, Rana (Odorrana) livida, by applying ‘Shotgun’ cloning method. The novel peptide was synthesised by using Solid Phase Peptide Synthesis strategy. After the synthetic peptide was identified and purified by Matrix-assisted, laser desorption, ionisation, time-offlight (MALDI-TOF) and Reversed-Phase High Performance Liquid Chromatography (RP-HPLC). QUB-2552 was subjected to functional tests and it was found to have moderate antimicrobial effect against C. albicans and S. aureus. Meanwhile, it has weak inhibitory effect against PC-3 and U251MG, but it did not exhibit haemolytic effect. It is expected that this study will contribute to the finding of novel AMPs and the design of novel antibiotics.","abstract_html":"Amphibian skin secretions are rich resources of bioactive peptides, some of which could be developed as drug candidates. Among these natural peptides, antimicrobial peptides have attracted great attention given that they might help to deal with the problem of antibiotic resistance. In this study, the precursor-encoding cDNA sequence of a novel bioactive peptide QUB-2552 was identified in the skin secretion of Chinese Large Odorous frog, Rana (Odorrana) livida, by applying ‘Shotgun’ cloning method. The novel peptide was synthesised by using Solid Phase Peptide Synthesis strategy. After the synthetic peptide was identified and purified by Matrix-assisted, laser desorption, ionisation, time-offlight (MALDI-TOF) and Reversed-Phase High Performance Liquid Chromatography (RP-HPLC). QUB-2552 was subjected to functional tests and it was found to have moderate antimicrobial effect against C. albicans and S. aureus. Meanwhile, it has weak inhibitory effect against PC-3 and U251MG, but it did not exhibit haemolytic effect. It is expected that this study will contribute to the finding of novel AMPs and the design of novel antibiotics.","abstract_has_math":false,"creators":["Zhang, Yujing"],"institution":"Queen's University Belfast","degree_name":"Master of Philosophy","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Chen, Tianbao","Shaw, Christopher","Zhou, Mei","Wang, Lei"],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-8","date_published":"2018-8","updated_at":"2026-07-24T03:54:53Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/1859873e-163d-4a3f-b989-ef1b1986b179"],"render_values":[{"text":"oai:pure.qub.ac.uk/portal:studenttheses/1859873e-163d-4a3f-b989-ef1b1986b179","href":null,"code":true}]}]},"links":{"outbound_url":"https://pure.qub.ac.uk/en/studentTheses/1859873e-163d-4a3f-b989-ef1b1986b179","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Chen, Tianbao","Shaw, Christopher","Zhou, Mei","Wang, Lei"]},{"key":"dc:creator","label":"Author","values":["Zhang, Yujing"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2018-8"]},{"key":"dc:date.issued","label":"Date","values":["2018-8"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Pharmacy"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Queen's University Belfast"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://pure.qub.ac.uk/en/studentTheses/1859873e-163d-4a3f-b989-ef1b1986b179"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Master of Philosophy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights.embargodate","label":"Dc Rights Embargodate","values":["2023-09-30"]},{"key":"dc:rights.embargoreason","label":"Dc Rights Embargoreason","values":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/1859873e-163d-4a3f-b989-ef1b1986b179","https://pure.qub.ac.uk/en/studentTheses/1859873e-163d-4a3f-b989-ef1b1986b179"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://pure.qub.ac.uk/files/157360483/Thesis_Zhang_Yujing_sumitted_version.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Amphibian skin secretions are rich resources of bioactive peptides, some of which could be developed as drug candidates. Among these natural peptides, antimicrobial peptides have attracted great attention given that they might help to deal with the problem of antibiotic resistance. In this study, the precursor-encoding cDNA sequence of a novel bioactive peptide QUB-2552 was identified in the skin secretion of Chinese Large Odorous frog, Rana (Odorrana) livida, by applying ‘Shotgun’ cloning method. The novel peptide was synthesised by using Solid Phase Peptide Synthesis strategy. After the synthetic peptide was identified and purified by Matrix-assisted, laser desorption, ionisation, time-offlight (MALDI-TOF) and Reversed-Phase High Performance Liquid Chromatography (RP-HPLC). QUB-2552 was subjected to functional tests and it was found to have moderate antimicrobial effect against C. albicans and S. aureus. Meanwhile, it has weak inhibitory effect against PC-3 and U251MG, but it did not exhibit haemolytic effect. It is expected that this study will contribute to the finding of novel AMPs and the design of novel antibiotics."]},{"key":"dc:title","label":"Title","values":["Bioactive evaluations of novel peptide QUB-2552 from the skin secretion of the Chinese Large Odorous frog, Rana (Odorrana) livida"]}]}],"canonical_facts":{"dc:contributor.advisor":["Chen, Tianbao","Shaw, Christopher","Zhou, Mei","Wang, Lei"],"dc:creator":["Zhang, Yujing"],"dc:date":["2018-8"],"dc:date.issued":["2018-8"],"dc:description.abstract":["Amphibian skin secretions are rich resources of bioactive peptides, some of which could be developed as drug candidates. Among these natural peptides, antimicrobial peptides have attracted great attention given that they might help to deal with the problem of antibiotic resistance. 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