{"id":{"repo_id":"qu-belfast","oai_identifier":"oai:pure.qub.ac.uk/portal:studenttheses/16e9b46b-c33d-4459-acec-934033d197dd"},"canonical_url":"https://search.dev.ndltd.org/etd/qu-belfast/oai:pure.qub.ac.uk/portal:studenttheses/16e9b46b-c33d-4459-acec-934033d197dd","repository":{"repo_id":"qu-belfast","name":"Queen's University Belfast","base_url":"https://pureadmin.qub.ac.uk/ws/oai"},"display":{"title":"Identification and functional study of a bioactive peptide QUB-2413 from the skin secretion of the Red-Eyed Tree Frog, Agalychnis callidryas","abstract":"Antimicrobial peptides secreted from amphibian skin secretions have many important bioactivities and act as the first line to defense against invading microorganisms. In this thesis, QUB-2413, a peptide belonging to the plasticins family of the dermaseptins superfamily, was derived from the skin secretion of the Central American Red-eyed Leaf Frog, Agalychnis callidryas. The peptide sequence was obtained by molecular cloning through constructing a cDNA library with isolated mRNA from frog skin secretion. The peptide was synthesised by solid phase peptide synthesiser, purified using reversed-phase high performance liquid chromatography and confirmed by MALDI-TOF. In broth microdilution assays, QUB-2413 showed no significant antimicrobial activities against the Gram-negative bacterium, Escherichia coli (E. coli ATCC CRM8739), and the yeast, Candida albicans (C. albicans ATCC CRM10231) at concentration of 512 µM, while it had inhibitory effects on the Gram-positive bacterium, Staphylococcus aureus (S. aureus ATCC CRM6538). In terms of MTT anticancer cell proliferation assays, QUB-2413 was proved to have no remarkable anticancer activities against U251-MG cells and HCT-116 cells in a range of concentration from 10 -6 M to 10 -4 M. With regard to haemolysis assay, QUB-2413 had no more than 10% haemolysis activity at 512 µM. Although the biological activities of QUB-2413 are not ideal, it is still expected to become a safe anti-positive bacteria drugs in the future after its antimicrobial activities get optimised through structural modification.","abstract_html":"Antimicrobial peptides secreted from amphibian skin secretions have many important bioactivities and act as the first line to defense against invading microorganisms. In this thesis, QUB-2413, a peptide belonging to the plasticins family of the dermaseptins superfamily, was derived from the skin secretion of the Central American Red-eyed Leaf Frog, Agalychnis callidryas. The peptide sequence was obtained by molecular cloning through constructing a cDNA library with isolated mRNA from frog skin secretion. The peptide was synthesised by solid phase peptide synthesiser, purified using reversed-phase high performance liquid chromatography and confirmed by MALDI-TOF. In broth microdilution assays, QUB-2413 showed no significant antimicrobial activities against the Gram-negative bacterium, Escherichia coli (E. coli ATCC CRM8739), and the yeast, Candida albicans (C. albicans ATCC CRM10231) at concentration of 512 µM, while it had inhibitory effects on the Gram-positive bacterium, Staphylococcus aureus (S. aureus ATCC CRM6538). In terms of MTT anticancer cell proliferation assays, QUB-2413 was proved to have no remarkable anticancer activities against U251-MG cells and HCT-116 cells in a range of concentration from 10 -6 M to 10 -4 M. With regard to haemolysis assay, QUB-2413 had no more than 10% haemolysis activity at 512 µM. Although the biological activities of QUB-2413 are not ideal, it is still expected to become a safe anti-positive bacteria drugs in the future after its antimicrobial activities get optimised through structural modification.","abstract_has_math":false,"creators":["Ren, Yue"],"institution":"Queen's University Belfast","degree_name":"Master of Philosophy","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Wang, Lei"],"committee_chairs":[],"committee_members":[],"year":2020,"date_issued":"2020-12","date_published":"2020-12","updated_at":"2026-07-24T03:55:31Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/16e9b46b-c33d-4459-acec-934033d197dd"],"render_values":[{"text":"oai:pure.qub.ac.uk/portal:studenttheses/16e9b46b-c33d-4459-acec-934033d197dd","href":null,"code":true}]}]},"links":{"outbound_url":"https://pure.qub.ac.uk/en/studentTheses/16e9b46b-c33d-4459-acec-934033d197dd","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Wang, Lei"]},{"key":"dc:creator","label":"Author","values":["Ren, Yue"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2020-12"]},{"key":"dc:date.issued","label":"Date","values":["2020-12"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Pharmacy"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Queen's University Belfast"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://pure.qub.ac.uk/en/studentTheses/16e9b46b-c33d-4459-acec-934033d197dd"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Master of Philosophy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights.embargodate","label":"Dc Rights Embargodate","values":["2025-12-31"]},{"key":"dc:rights.embargoreason","label":"Dc Rights Embargoreason","values":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/16e9b46b-c33d-4459-acec-934033d197dd","https://pure.qub.ac.uk/en/studentTheses/16e9b46b-c33d-4459-acec-934033d197dd"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://pure.qub.ac.uk/files/220068605/Thesis_RenYue.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Antimicrobial peptides secreted from amphibian skin secretions have many important bioactivities and act as the first line to defense against invading microorganisms. In this thesis, QUB-2413, a peptide belonging to the plasticins family of the dermaseptins superfamily, was derived from the skin secretion of the Central American Red-eyed Leaf Frog, Agalychnis callidryas. The peptide sequence was obtained by molecular cloning through constructing a cDNA library with isolated mRNA from frog skin secretion. The peptide was synthesised by solid phase peptide synthesiser, purified using reversed-phase high performance liquid chromatography and confirmed by MALDI-TOF. In broth microdilution assays, QUB-2413 showed no significant antimicrobial activities against the Gram-negative bacterium, Escherichia coli (E. coli ATCC CRM8739), and the yeast, Candida albicans (C. albicans ATCC CRM10231) at concentration of 512 µM, while it had inhibitory effects on the Gram-positive bacterium, Staphylococcus aureus (S. aureus ATCC CRM6538). In terms of MTT anticancer cell proliferation assays, QUB-2413 was proved to have no remarkable anticancer activities against U251-MG cells and HCT-116 cells in a range of concentration from 10 -6 M to 10 -4 M. With regard to haemolysis assay, QUB-2413 had no more than 10% haemolysis activity at 512 µM. Although the biological activities of QUB-2413 are not ideal, it is still expected to become a safe anti-positive bacteria drugs in the future after its antimicrobial activities get optimised through structural modification."]},{"key":"dc:title","label":"Title","values":["Identification and functional study of a bioactive peptide QUB-2413 from the skin secretion of the Red-Eyed Tree Frog, Agalychnis callidryas"]}]}],"canonical_facts":{"dc:contributor.advisor":["Wang, Lei"],"dc:creator":["Ren, Yue"],"dc:date":["2020-12"],"dc:date.issued":["2020-12"],"dc:description.abstract":["Antimicrobial peptides secreted from amphibian skin secretions have many important bioactivities and act as the first line to defense against invading microorganisms. In this thesis, QUB-2413, a peptide belonging to the plasticins family of the dermaseptins superfamily, was derived from the skin secretion of the Central American Red-eyed Leaf Frog, Agalychnis callidryas. The peptide sequence was obtained by molecular cloning through constructing a cDNA library with isolated mRNA from frog skin secretion. The peptide was synthesised by solid phase peptide synthesiser, purified using reversed-phase high performance liquid chromatography and confirmed by MALDI-TOF. In broth microdilution assays, QUB-2413 showed no significant antimicrobial activities against the Gram-negative bacterium, Escherichia coli (E. coli ATCC CRM8739), and the yeast, Candida albicans (C. albicans ATCC CRM10231) at concentration of 512 µM, while it had inhibitory effects on the Gram-positive bacterium, Staphylococcus aureus (S. aureus ATCC CRM6538). In terms of MTT anticancer cell proliferation assays, QUB-2413 was proved to have no remarkable anticancer activities against U251-MG cells and HCT-116 cells in a range of concentration from 10 -6 M to 10 -4 M. With regard to haemolysis assay, QUB-2413 had no more than 10% haemolysis activity at 512 µM. Although the biological activities of QUB-2413 are not ideal, it is still expected to become a safe anti-positive bacteria drugs in the future after its antimicrobial activities get optimised through structural modification."],"dc:identifier":["oai:pure.qub.ac.uk/portal:studenttheses/16e9b46b-c33d-4459-acec-934033d197dd","https://pure.qub.ac.uk/en/studentTheses/16e9b46b-c33d-4459-acec-934033d197dd"],"dc:identifier.uri":["https://pure.qub.ac.uk/files/220068605/Thesis_RenYue.pdf"],"dc:language":["eng"],"dc:publisher.department":["School of Pharmacy"],"dc:publisher.institution":["Queen's University Belfast"],"dc:relation.isreferencedby":["https://pure.qub.ac.uk/en/studentTheses/16e9b46b-c33d-4459-acec-934033d197dd"],"dc:rights.embargodate":["2025-12-31"],"dc:rights.embargoreason":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"],"dc:title":["Identification and functional study of a bioactive peptide QUB-2413 from the skin secretion of the Red-Eyed Tree Frog, Agalychnis callidryas"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["Masters Thesis"],"dc:type.qualificationname":["Master of Philosophy"]},"updated_at":"2026-07-24T03:55:31Z"}