{"id":{"repo_id":"qu-belfast","oai_identifier":"oai:pure.qub.ac.uk/portal:studenttheses/0b4abde3-706b-4e81-a61a-990d1c9eff66"},"canonical_url":"https://search.dev.ndltd.org/etd/qu-belfast/oai:pure.qub.ac.uk/portal:studenttheses/0b4abde3-706b-4e81-a61a-990d1c9eff66","repository":{"repo_id":"qu-belfast","name":"Queen's University Belfast","base_url":"https://pureadmin.qub.ac.uk/ws/oai"},"display":{"title":"Identification and bioactivity assessment of an antimicrobial peptide from the defensive skin secretion of the northern leopard frog, Rana pipiens","abstract":"In response to the growing threat of bacterial resistance, antimicrobial peptides (AMPs) have emerged due to their broad-spectrum activity and low probability of resistance generation. In many ways, antimicrobial peptides have advantages that are difficult to match with antibiotics, and so research into them continues to intensify.<br/><br/>In the present work, a novel AMP belonging to the Brevinin family was isolated from the skin secretions of Rana pipiens. After cDNA library construction and gene sequencing of mRNA from skin secretions, a sequence of a mature peptide was identified. After synthesis, identification and purification steps, the peptide sequence was tested for biological activity, including antibacterial, anticancer and haemolytic.<br/><br/>As a result of the above experimental steps, the sequence of the mature peptide was identified as: FLPIIAGVAAKVFPKIFCTISKKC. The peptide sequence was identified as a known antimicrobial peptide named Brevinin-1Pk. In bioactivity assays, this peptide was found to be significantly inhibitory to Gram-positive and Gram-negative bacteria and yeast (Staphylococcus aureus and Escherichia coli and Candida albicans, respectively.) at concentrations of 2 µM, 16 µM and 32 µM respectively, and anticancer activity was found at 100 µM with an IC50 of 2.822 * 10-5 M. At the same time, this peptide performed poorly in the haemolytic assay, showing significant haemolysis at a concentration of only 32 µM.<br/><br/>Based on the experimental results, Brevinin-1Pk has been shown to have potential as a clinical antibacterial agent. The haemolytic activity of Brevinin-1Pk is one to two orders of magnitude higher than that of antimicrobial drugs already in clinical use, and therefore it should probably be used as a topical agent for clinical purposes.<br/><br/><i>Thesis embargoed until 31 December 2026</i>.","abstract_html":"In response to the growing threat of bacterial resistance, antimicrobial peptides (AMPs) have emerged due to their broad-spectrum activity and low probability of resistance generation. In many ways, antimicrobial peptides have advantages that are difficult to match with antibiotics, and so research into them continues to intensify.&lt;br/&gt;&lt;br/&gt;In the present work, a novel AMP belonging to the Brevinin family was isolated from the skin secretions of Rana pipiens. After cDNA library construction and gene sequencing of mRNA from skin secretions, a sequence of a mature peptide was identified. After synthesis, identification and purification steps, the peptide sequence was tested for biological activity, including antibacterial, anticancer and haemolytic.&lt;br/&gt;&lt;br/&gt;As a result of the above experimental steps, the sequence of the mature peptide was identified as: FLPIIAGVAAKVFPKIFCTISKKC. The peptide sequence was identified as a known antimicrobial peptide named Brevinin-1Pk. In bioactivity assays, this peptide was found to be significantly inhibitory to Gram-positive and Gram-negative bacteria and yeast (Staphylococcus aureus and Escherichia coli and Candida albicans, respectively.) at concentrations of 2 µM, 16 µM and 32 µM respectively, and anticancer activity was found at 100 µM with an IC50 of 2.822 * 10-5 M. At the same time, this peptide performed poorly in the haemolytic assay, showing significant haemolysis at a concentration of only 32 µM.&lt;br/&gt;&lt;br/&gt;Based on the experimental results, Brevinin-1Pk has been shown to have potential as a clinical antibacterial agent. The haemolytic activity of Brevinin-1Pk is one to two orders of magnitude higher than that of antimicrobial drugs already in clinical use, and therefore it should probably be used as a topical agent for clinical purposes.&lt;br/&gt;&lt;br/&gt;&lt;i&gt;Thesis embargoed until 31 December 2026&lt;/i&gt;.","abstract_has_math":false,"creators":["Huang, Zhimin"],"institution":"Queen's University Belfast","degree_name":"Master of Philosophy","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Zhou, Mei","Wang, Lei","Chen, Tianbao"],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-12","date_published":"2021-12","updated_at":"2026-07-24T03:56:04Z","subjects":["Antimicrobial peptide","brevinin superfamily","antimicrobial activity","anti-profilication activity","haemolytic activity"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/0b4abde3-706b-4e81-a61a-990d1c9eff66"],"render_values":[{"text":"oai:pure.qub.ac.uk/portal:studenttheses/0b4abde3-706b-4e81-a61a-990d1c9eff66","href":null,"code":true}]}]},"links":{"outbound_url":"https://pure.qub.ac.uk/en/studentTheses/0b4abde3-706b-4e81-a61a-990d1c9eff66","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Zhou, Mei","Wang, Lei","Chen, Tianbao"]},{"key":"dc:creator","label":"Author","values":["Huang, Zhimin"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2021-12"]},{"key":"dc:date.issued","label":"Date","values":["2021-12"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Pharmacy"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Queen's University Belfast"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://pure.qub.ac.uk/en/studentTheses/0b4abde3-706b-4e81-a61a-990d1c9eff66"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Master of Philosophy"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Antimicrobial peptide","brevinin superfamily","antimicrobial activity","anti-profilication activity","haemolytic activity"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights.embargodate","label":"Dc Rights Embargodate","values":["2026-12-31"]},{"key":"dc:rights.embargoreason","label":"Dc Rights Embargoreason","values":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/0b4abde3-706b-4e81-a61a-990d1c9eff66","https://pure.qub.ac.uk/en/studentTheses/0b4abde3-706b-4e81-a61a-990d1c9eff66"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["In response to the growing threat of bacterial resistance, antimicrobial peptides (AMPs) have emerged due to their broad-spectrum activity and low probability of resistance generation. In many ways, antimicrobial peptides have advantages that are difficult to match with antibiotics, and so research into them continues to intensify.<br/><br/>In the present work, a novel AMP belonging to the Brevinin family was isolated from the skin secretions of Rana pipiens. After cDNA library construction and gene sequencing of mRNA from skin secretions, a sequence of a mature peptide was identified. After synthesis, identification and purification steps, the peptide sequence was tested for biological activity, including antibacterial, anticancer and haemolytic.<br/><br/>As a result of the above experimental steps, the sequence of the mature peptide was identified as: FLPIIAGVAAKVFPKIFCTISKKC. The peptide sequence was identified as a known antimicrobial peptide named Brevinin-1Pk. In bioactivity assays, this peptide was found to be significantly inhibitory to Gram-positive and Gram-negative bacteria and yeast (Staphylococcus aureus and Escherichia coli and Candida albicans, respectively.) at concentrations of 2 µM, 16 µM and 32 µM respectively, and anticancer activity was found at 100 µM with an IC50 of 2.822 * 10-5 M. At the same time, this peptide performed poorly in the haemolytic assay, showing significant haemolysis at a concentration of only 32 µM.<br/><br/>Based on the experimental results, Brevinin-1Pk has been shown to have potential as a clinical antibacterial agent. The haemolytic activity of Brevinin-1Pk is one to two orders of magnitude higher than that of antimicrobial drugs already in clinical use, and therefore it should probably be used as a topical agent for clinical purposes.<br/><br/><i>Thesis embargoed until 31 December 2026</i>."]},{"key":"dc:title","label":"Title","values":["Identification and bioactivity assessment of an antimicrobial peptide from the defensive skin secretion of the northern leopard frog, Rana pipiens"]}]}],"canonical_facts":{"dc:contributor.advisor":["Zhou, Mei","Wang, Lei","Chen, Tianbao"],"dc:creator":["Huang, Zhimin"],"dc:date":["2021-12"],"dc:date.issued":["2021-12"],"dc:description.abstract":["In response to the growing threat of bacterial resistance, antimicrobial peptides (AMPs) have emerged due to their broad-spectrum activity and low probability of resistance generation. In many ways, antimicrobial peptides have advantages that are difficult to match with antibiotics, and so research into them continues to intensify.<br/><br/>In the present work, a novel AMP belonging to the Brevinin family was isolated from the skin secretions of Rana pipiens. After cDNA library construction and gene sequencing of mRNA from skin secretions, a sequence of a mature peptide was identified. After synthesis, identification and purification steps, the peptide sequence was tested for biological activity, including antibacterial, anticancer and haemolytic.<br/><br/>As a result of the above experimental steps, the sequence of the mature peptide was identified as: FLPIIAGVAAKVFPKIFCTISKKC. The peptide sequence was identified as a known antimicrobial peptide named Brevinin-1Pk. In bioactivity assays, this peptide was found to be significantly inhibitory to Gram-positive and Gram-negative bacteria and yeast (Staphylococcus aureus and Escherichia coli and Candida albicans, respectively.) at concentrations of 2 µM, 16 µM and 32 µM respectively, and anticancer activity was found at 100 µM with an IC50 of 2.822 * 10-5 M. At the same time, this peptide performed poorly in the haemolytic assay, showing significant haemolysis at a concentration of only 32 µM.<br/><br/>Based on the experimental results, Brevinin-1Pk has been shown to have potential as a clinical antibacterial agent. The haemolytic activity of Brevinin-1Pk is one to two orders of magnitude higher than that of antimicrobial drugs already in clinical use, and therefore it should probably be used as a topical agent for clinical purposes.<br/><br/><i>Thesis embargoed until 31 December 2026</i>."],"dc:identifier":["oai:pure.qub.ac.uk/portal:studenttheses/0b4abde3-706b-4e81-a61a-990d1c9eff66","https://pure.qub.ac.uk/en/studentTheses/0b4abde3-706b-4e81-a61a-990d1c9eff66"],"dc:language":["eng"],"dc:publisher.department":["School of Pharmacy"],"dc:publisher.institution":["Queen's University Belfast"],"dc:relation.isreferencedby":["https://pure.qub.ac.uk/en/studentTheses/0b4abde3-706b-4e81-a61a-990d1c9eff66"],"dc:rights.embargodate":["2026-12-31"],"dc:rights.embargoreason":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"],"dc:subject":["Antimicrobial peptide","brevinin superfamily","antimicrobial activity","anti-profilication activity","haemolytic activity"],"dc:title":["Identification and bioactivity assessment of an antimicrobial peptide from the defensive skin secretion of the northern leopard frog, Rana pipiens"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["Masters Thesis"],"dc:type.qualificationname":["Master of Philosophy"]},"updated_at":"2026-07-24T03:56:04Z"}