Biological activities and antidiabetic potential of novel peptides from frog secretions
type: CreativeWork
description: The utilisation of natural products as drugs for the treatment of diseases and injuries has developed for thousands of years, but the current pharmaceutical system is predominately occupied by chemical-based drugs, while natural products are rarely applied. Since the widespread of antibiotic resistance and prevalence of chronic and malignant diseases (such as diabetes and cancers), there has been a significant rise of interest in discovering novel drug candidates with potent efficiency in treating these diseases. The amphibian skin, a special morphological and physiological organ, acts as the first line of defence against physical stimuli and lethal invasions. It is a rich source of pharmacological alternatives, some of which possess the potential to be developed in medical applications. In this study, both genomics and proteomics techniques have been used to study the frog skin secretions from the Burmeister’s leaf frog, Phyllomedusa burmeisteri; the hylid frog, Phyllomedusa sauvagei and the Chinese tiger frog, Hoplobatrachus rugulosus. More specifically, the molecular cloning strategy was performed to isolate target mRNAs, establish corresponding cDNA libraries and eventually clone unique peptide precursor-encoding cDNA sequences. Also, the mature peptides were analysed and confirmed via RP-HPLC, MALDI-TOF mass spectrometry and MS/MS fragmentation sequencing. After the peptide primary structure characterisation, synthetic replicates of each peptide were purified before applied for further secondary structural analysis and biofunctional assessments. The secondary structure conformations were determined by circular dichroism, and the functional analysis in this study includes the antimicrobial assay, MTT assay, haemolysis assay, insulinotropic assay and GLP-1 releasing assay. This study reports the isolation, identification and characterisation of one phylloseptin peptide Phylloseptin-PBu, one dermaseptin peptide Dermaseptin-PS1 and four tigerinin peptides (Tigerini…
abstract: The utilisation of natural products as drugs for the treatment of diseases and injuries has developed for thousands of years, but the current pharmaceutical system is predominately occupied by chemical-based drugs, while natural products are rarely applied. Since the widespread of antibiotic resistance and prevalence of chronic and malignant diseases (such as diabetes and cancers), there has been a significant rise of interest in discovering novel drug candidates with potent efficiency in treating these diseases. The amphibian skin, a special morphological and physiological organ, acts as the first line of defence against physical stimuli and lethal invasions. It is a rich source of pharmacological alternatives, some of which possess the potential to be developed in medical applications. In this study, both genomics and proteomics techniques have been used to study the frog skin secretions from the Burmeister’s leaf frog, Phyllomedusa burmeisteri; the hylid frog, Phyllomedusa sauvagei and the Chinese tiger frog, Hoplobatrachus rugulosus. More specifically, the molecular cloning strategy was performed to isolate target mRNAs, establish corresponding cDNA libraries and eventually clone unique peptide precursor-encoding cDNA sequences. Also, the mature peptides were analysed and confirmed via RP-HPLC, MALDI-TOF mass spectrometry and MS/MS fragmentation sequencing. After the peptide primary structure characterisation, synthetic replicates of each peptide were purified before applied for further secondary structural analysis and biofunctional assessments. The secondary structure conformations were determined by circular dichroism, and the functional analysis in this study includes the antimicrobial assay, MTT assay, haemolysis assay, insulinotropic assay and GLP-1 releasing assay. This study reports the isolation, identification and characterisation of one phylloseptin peptide Phylloseptin-PBu, one dermaseptin peptide Dermaseptin-PS1 and four tigerinin peptides (Tigerini…
author: Long, Qilin
language: eng
date published: 2018-12
date modified: 2026-07-24T03:54:53Z
provider: Queen's University Belfast
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Queen's University Belfast