{"id":{"repo_id":"purdue-thes","oai_identifier":"oai:docs.lib.purdue.edu:open_access_dissertations-2502"},"canonical_url":"https://search.dev.ndltd.org/etd/purdue-thes/oai:docs.lib.purdue.edu:open_access_dissertations-2502","repository":{"repo_id":"purdue-thes","name":"Purdue University","base_url":"https://docs.lib.purdue.edu/do/oai/"},"display":{"title":"HOOKLESS1 REGULATES RESPONSES TO PATHOGENS AND ABSCISIC ACID THROUGH INTERACTION WITH MEDIATOR18 AND ACETYLATION OF WRKY33 AND ABI5 CHROMATIN","abstract":"Arabidopsis histone acetyltransferase HLS1 has been implicated in the control of seedling development in response to ethylene. Here, we demonstrate that the loss of function hls1 mutant plants have broad effects on the plant development such as early senescence and flowering, seed insensitivity to ABA as well as plant defense responses including enhanced susceptibility to the necrotrophic pathogen Botrytis cinerea and the hemi-biotrophic pathogen Pseudomonas syringae DC3000 strain expressing the effector protein AvrRpm1 strain. We show that HLS1 modulates the expression of WRKY33 gene, a known defense regulator, as well as ABI5 in modulating ABA signaling pathway. The regulation of WRKY33 and ABI5 is through histone H3 acetylation (H3Ac) at the specific loci. Furthermore, pathogen infection enhances HLS1 association and H3Ac at WRKY33 chromatin.","abstract_html":"Arabidopsis histone acetyltransferase HLS1 has been implicated in the control of seedling development in response to ethylene. Here, we demonstrate that the loss of function hls1 mutant plants have broad effects on the plant development such as early senescence and flowering, seed insensitivity to ABA as well as plant defense responses including enhanced susceptibility to the necrotrophic pathogen Botrytis cinerea and the hemi-biotrophic pathogen Pseudomonas syringae DC3000 strain expressing the effector protein AvrRpm1 strain. We show that HLS1 modulates the expression of WRKY33 gene, a known defense regulator, as well as ABI5 in modulating ABA signaling pathway. The regulation of WRKY33 and ABI5 is through histone H3 acetylation (H3Ac) at the specific loci. Furthermore, pathogen infection enhances HLS1 association and H3Ac at WRKY33 chromatin.","abstract_has_math":false,"creators":["Liao, Chao-Jan"],"institution":null,"degree_name":"Doctor of Philosophy (PhD)","degree_level":"Dissertation","degree_discipline":"Botany and Plant Pathology","degree_department":null,"school":null,"contributors":["Tesfaye Mengiste","Jin-Rong Xu","Gurmukh Johal","Steven Scofield"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-01-01T08:00:00Z","date_published":"2016-01-01T08:00:00Z","updated_at":"2026-07-24T03:54:31Z","subjects":[],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://docs.lib.purdue.edu/open_access_dissertations/1286","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Tesfaye Mengiste","Jin-Rong Xu","Gurmukh Johal","Steven Scofield"]},{"key":"dc:creator","label":"Author","values":["Liao, Chao-Jan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Botany and Plant Pathology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy (PhD)"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://docs.lib.purdue.edu/open_access_dissertations/1286"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Arabidopsis histone acetyltransferase HLS1 has been implicated in the control of seedling development in response to ethylene. Here, we demonstrate that the loss of function hls1 mutant plants have broad effects on the plant development such as early senescence and flowering, seed insensitivity to ABA as well as plant defense responses including enhanced susceptibility to the necrotrophic pathogen Botrytis cinerea and the hemi-biotrophic pathogen Pseudomonas syringae DC3000 strain expressing the effector protein AvrRpm1 strain. We show that HLS1 modulates the expression of WRKY33 gene, a known defense regulator, as well as ABI5 in modulating ABA signaling pathway. The regulation of WRKY33 and ABI5 is through histone H3 acetylation (H3Ac) at the specific loci. Furthermore, pathogen infection enhances HLS1 association and H3Ac at WRKY33 chromatin."]},{"key":"dc:title","label":"Title","values":["HOOKLESS1 REGULATES RESPONSES TO PATHOGENS AND ABSCISIC ACID THROUGH INTERACTION WITH MEDIATOR18 AND ACETYLATION OF WRKY33 AND ABI5 CHROMATIN"]}]}],"canonical_facts":{"dc:contributor":["Tesfaye Mengiste","Jin-Rong Xu","Gurmukh Johal","Steven Scofield"],"dc:creator":["Liao, Chao-Jan"],"dc:description.abstract":["Arabidopsis histone acetyltransferase HLS1 has been implicated in the control of seedling development in response to ethylene. Here, we demonstrate that the loss of function hls1 mutant plants have broad effects on the plant development such as early senescence and flowering, seed insensitivity to ABA as well as plant defense responses including enhanced susceptibility to the necrotrophic pathogen Botrytis cinerea and the hemi-biotrophic pathogen Pseudomonas syringae DC3000 strain expressing the effector protein AvrRpm1 strain. We show that HLS1 modulates the expression of WRKY33 gene, a known defense regulator, as well as ABI5 in modulating ABA signaling pathway. The regulation of WRKY33 and ABI5 is through histone H3 acetylation (H3Ac) at the specific loci. Furthermore, pathogen infection enhances HLS1 association and H3Ac at WRKY33 chromatin."],"dc:identifier":["https://docs.lib.purdue.edu/open_access_dissertations/1286"],"dc:title":["HOOKLESS1 REGULATES RESPONSES TO PATHOGENS AND ABSCISIC ACID THROUGH INTERACTION WITH MEDIATOR18 AND ACETYLATION OF WRKY33 AND ABI5 CHROMATIN"],"thesis:degree_discipline":["Botany and Plant Pathology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Doctor of Philosophy (PhD)"]},"updated_at":"2026-07-24T03:54:31Z"}