{"id":{"repo_id":"purdue-thes","oai_identifier":"oai:docs.lib.purdue.edu:open_access_dissertations-2287"},"canonical_url":"https://search.dev.ndltd.org/etd/purdue-thes/oai:docs.lib.purdue.edu:open_access_dissertations-2287","repository":{"repo_id":"purdue-thes","name":"Purdue University","base_url":"https://docs.lib.purdue.edu/do/oai/"},"display":{"title":"NOVEL STRATEGIES TO DEVELOP BETTER BRUCELLOSIS VACCINES USING BRUCELLA ABORTUS RB51 AND B. NEOTOMAE","abstract":"The genus Brucella consists of Gram-negative, facultative intracellular coccobacilli that can cause chronic infections in several mammals. Brucella spp. can exhibit a smooth or rough phenotype; smooth Brucella spp. contain a surface-exposed O-polysaccharide in their cell wall structure while the rough Brucella spp. are devoid of the O-polysaccharide. Acquired immunity against Brucella infection is primarily cell-mediated and involves both CD4+ T cells and CD8+ T cell responses. However, antibodies to the O-polysaccharide also play a role in enhancing the protection against infections by virulent Brucella species in some hosts. B. abortus strain RB51 is a stable rough attenuated mutant which is used as a licensed live vaccine for bovine brucellosis in the United States and several other countries. Previous studies have shown that the wboA gene, which encodes a glycosyltransferase required for the synthesis of O-polysaccharide in Brucella, is disrupted in B. abortus RB51 by an IS711 element. Although low-levels of intra-cytoplasmic O-polysaccharide were produced when RB51 was complemented with a functional wboA gene (strain RB51WboA), it did not result in a smooth phenotype. This suggests that mutations in several genes of the O-polysaccharide biosynthesis pathway contribute to the rough phenotype of RB51. However, nucleotide sequence analysis has revealed that there are no other gene-disrupting mutations that could affect the smooth LPS synthesis in strain RB51.","abstract_html":"The genus Brucella consists of Gram-negative, facultative intracellular coccobacilli that can cause chronic infections in several mammals. Brucella spp. can exhibit a smooth or rough phenotype; smooth Brucella spp. contain a surface-exposed O-polysaccharide in their cell wall structure while the rough Brucella spp. are devoid of the O-polysaccharide. Acquired immunity against Brucella infection is primarily cell-mediated and involves both CD4+ T cells and CD8+ T cell responses. However, antibodies to the O-polysaccharide also play a role in enhancing the protection against infections by virulent Brucella species in some hosts. B. abortus strain RB51 is a stable rough attenuated mutant which is used as a licensed live vaccine for bovine brucellosis in the United States and several other countries. Previous studies have shown that the wboA gene, which encodes a glycosyltransferase required for the synthesis of O-polysaccharide in Brucella, is disrupted in B. abortus RB51 by an IS711 element. Although low-levels of intra-cytoplasmic O-polysaccharide were produced when RB51 was complemented with a functional wboA gene (strain RB51WboA), it did not result in a smooth phenotype. This suggests that mutations in several genes of the O-polysaccharide biosynthesis pathway contribute to the rough phenotype of RB51. However, nucleotide sequence analysis has revealed that there are no other gene-disrupting mutations that could affect the smooth LPS synthesis in strain RB51.","abstract_has_math":false,"creators":["Dabral, Neha"],"institution":null,"degree_name":"Doctor of Philosophy (PhD)","degree_level":"Dissertation","degree_discipline":"Comparative Pathobiology","degree_department":null,"school":null,"contributors":["Ramesh Vemulapalli","Harm HogenEsch","Carolyn Guptill-Yoran","Mohamed Seleem"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-01-01T08:00:00Z","date_published":"2014-01-01T08:00:00Z","updated_at":"2026-07-24T03:54:17Z","subjects":["Brucella","Exopolysaccharide","Gamma irradiation","Overexpression","Prime-boost","Vaccine"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://docs.lib.purdue.edu/open_access_dissertations/1071","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Ramesh Vemulapalli","Harm HogenEsch","Carolyn Guptill-Yoran","Mohamed Seleem"]},{"key":"dc:creator","label":"Author","values":["Dabral, Neha"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Comparative Pathobiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy (PhD)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Brucella","Exopolysaccharide","Gamma irradiation","Overexpression","Prime-boost","Vaccine"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://docs.lib.purdue.edu/open_access_dissertations/1071"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The genus Brucella consists of Gram-negative, facultative intracellular coccobacilli that can cause chronic infections in several mammals. Brucella spp. can exhibit a smooth or rough phenotype; smooth Brucella spp. contain a surface-exposed O-polysaccharide in their cell wall structure while the rough Brucella spp. are devoid of the O-polysaccharide. Acquired immunity against Brucella infection is primarily cell-mediated and involves both CD4+ T cells and CD8+ T cell responses. However, antibodies to the O-polysaccharide also play a role in enhancing the protection against infections by virulent Brucella species in some hosts. B. abortus strain RB51 is a stable rough attenuated mutant which is used as a licensed live vaccine for bovine brucellosis in the United States and several other countries. Previous studies have shown that the wboA gene, which encodes a glycosyltransferase required for the synthesis of O-polysaccharide in Brucella, is disrupted in B. abortus RB51 by an IS711 element. Although low-levels of intra-cytoplasmic O-polysaccharide were produced when RB51 was complemented with a functional wboA gene (strain RB51WboA), it did not result in a smooth phenotype. This suggests that mutations in several genes of the O-polysaccharide biosynthesis pathway contribute to the rough phenotype of RB51. However, nucleotide sequence analysis has revealed that there are no other gene-disrupting mutations that could affect the smooth LPS synthesis in strain RB51."]},{"key":"dc:title","label":"Title","values":["NOVEL STRATEGIES TO DEVELOP BETTER BRUCELLOSIS VACCINES USING BRUCELLA ABORTUS RB51 AND B. NEOTOMAE"]}]}],"canonical_facts":{"dc:contributor":["Ramesh Vemulapalli","Harm HogenEsch","Carolyn Guptill-Yoran","Mohamed Seleem"],"dc:creator":["Dabral, Neha"],"dc:description.abstract":["The genus Brucella consists of Gram-negative, facultative intracellular coccobacilli that can cause chronic infections in several mammals. Brucella spp. can exhibit a smooth or rough phenotype; smooth Brucella spp. contain a surface-exposed O-polysaccharide in their cell wall structure while the rough Brucella spp. are devoid of the O-polysaccharide. Acquired immunity against Brucella infection is primarily cell-mediated and involves both CD4+ T cells and CD8+ T cell responses. However, antibodies to the O-polysaccharide also play a role in enhancing the protection against infections by virulent Brucella species in some hosts. B. abortus strain RB51 is a stable rough attenuated mutant which is used as a licensed live vaccine for bovine brucellosis in the United States and several other countries. Previous studies have shown that the wboA gene, which encodes a glycosyltransferase required for the synthesis of O-polysaccharide in Brucella, is disrupted in B. abortus RB51 by an IS711 element. Although low-levels of intra-cytoplasmic O-polysaccharide were produced when RB51 was complemented with a functional wboA gene (strain RB51WboA), it did not result in a smooth phenotype. This suggests that mutations in several genes of the O-polysaccharide biosynthesis pathway contribute to the rough phenotype of RB51. However, nucleotide sequence analysis has revealed that there are no other gene-disrupting mutations that could affect the smooth LPS synthesis in strain RB51."],"dc:identifier":["https://docs.lib.purdue.edu/open_access_dissertations/1071"],"dc:subject":["Brucella","Exopolysaccharide","Gamma irradiation","Overexpression","Prime-boost","Vaccine"],"dc:title":["NOVEL STRATEGIES TO DEVELOP BETTER BRUCELLOSIS VACCINES USING BRUCELLA ABORTUS RB51 AND B. NEOTOMAE"],"thesis:degree_discipline":["Comparative Pathobiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Doctor of Philosophy (PhD)"]},"updated_at":"2026-07-24T03:54:17Z"}