Assessing gene expression of coagulation factors and Factor XIII polymorphisms in type 2 diabetes mellitus patients
type: CreativeWork
description: Background: Type 2 diabetes mellitus (T2DM) is characterized by chronic hyperglycaemia, inflammation and coagulopathies due to cytokine activation of IL-1_ and IL-6. The aim of the study was to assess the mRNA levels of coagulation factors and the identification of two FXIII SNPs (Val34Leu and Tyr204Phe) in T2DM patients (n=100) compared to controls (n=100). Methodology: Thromboelastography (TEG) was used to assess the viscoelastic properties of pre-selected clot parameters. Quantitative PCR (qPCR) was used to assess mRNA levels of key coagulation factors (factor XIII-A, factor XII and tissue factor). PCR-Restriction fragment length polymorphism (PCR-RFLP) was used to identify the presence or absence of Val34Leu and Tyr204Phe using specific restriction enzymes. Results: From the pre-selected viscoelastic properties, TEG showed that the R time and TMRTG were prolonged by 17.67 mins (26.15±2.56 min vs. 8.48±0,92 min) and 12.76 mins (24.85±2.41 min vs. 12.09±1.15 min) (p<0.0001) respectively. There was a reduction in MA by 2.55 dyn.cm-2s-1 (3.94±0.61 dyn.cm_2 s_1 vs. 5.73±0.54 dyn.cm_2 s_1, p=0.0074) and TTG by 121 dyn.cm-2 (213.8±26.29 dyn cm_2 vs. 335.5 ± 32.33 dyn cm_2, p= 0.0385) in T2DM patients compared to controls. A 4.38-fold decrease in mRNA levels of FXIII-A in T2DM (delta ct: 10.94±0.44) was observed, compared to controls (delta ct: 13.07±0.48), p=0.0033. The genotype distribution in both T2DM and control individuals conformed to the Hardy-Weinberg Equilibrium (HWE) (p>0.05). An association of the Val34Leu SNP and T2DM was found, (OR) = 1.670, 95% CI=0.97-2.89, p=0.0747) . An even stronger association was found with the Tyr204Phe SNP and T2DM (OR=3.57, 95% CI=2.37-5.45, p<0.0001 ). The levels of FXIII-A mRNA was significantly altered in T2DM (delta ct: 14.46±0.82 vs. 12.03±0.49, p=0.0105) in the presence of the wild type (Tyr/Tyr) compared to the variant (Tyr/Phe and Phe/Phe), respectively. The polymorphism had an effect on the viscoelastic properties as sho…
abstract: Background: Type 2 diabetes mellitus (T2DM) is characterized by chronic hyperglycaemia, inflammation and coagulopathies due to cytokine activation of IL-1_ and IL-6. The aim of the study was to assess the mRNA levels of coagulation factors and the identification of two FXIII SNPs (Val34Leu and Tyr204Phe) in T2DM patients (n=100) compared to controls (n=100). Methodology: Thromboelastography (TEG) was used to assess the viscoelastic properties of pre-selected clot parameters. Quantitative PCR (qPCR) was used to assess mRNA levels of key coagulation factors (factor XIII-A, factor XII and tissue factor). PCR-Restriction fragment length polymorphism (PCR-RFLP) was used to identify the presence or absence of Val34Leu and Tyr204Phe using specific restriction enzymes. Results: From the pre-selected viscoelastic properties, TEG showed that the R time and TMRTG were prolonged by 17.67 mins (26.15±2.56 min vs. 8.48±0,92 min) and 12.76 mins (24.85±2.41 min vs. 12.09±1.15 min) (p<0.0001) respectively. There was a reduction in MA by 2.55 dyn.cm-2s-1 (3.94±0.61 dyn.cm_2 s_1 vs. 5.73±0.54 dyn.cm_2 s_1, p=0.0074) and TTG by 121 dyn.cm-2 (213.8±26.29 dyn cm_2 vs. 335.5 ± 32.33 dyn cm_2, p= 0.0385) in T2DM patients compared to controls. A 4.38-fold decrease in mRNA levels of FXIII-A in T2DM (delta ct: 10.94±0.44) was observed, compared to controls (delta ct: 13.07±0.48), p=0.0033. The genotype distribution in both T2DM and control individuals conformed to the Hardy-Weinberg Equilibrium (HWE) (p>0.05). An association of the Val34Leu SNP and T2DM was found, (OR) = 1.670, 95% CI=0.97-2.89, p=0.0747) . An even stronger association was found with the Tyr204Phe SNP and T2DM (OR=3.57, 95% CI=2.37-5.45, p<0.0001 ). The levels of FXIII-A mRNA was significantly altered in T2DM (delta ct: 14.46±0.82 vs. 12.03±0.49, p=0.0105) in the presence of the wild type (Tyr/Tyr) compared to the variant (Tyr/Phe and Phe/Phe), respectively. The polymorphism had an effect on the viscoelastic properties as sho…
language: en
keywords: UCTD
date published: 2018
date modified: 2026-07-24T03:52:32Z
provider: University of Pretoria
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Pretoria

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property ID: Handle
value: http://hdl.handle.net/2263/69973