Biological activity of Sinularia notanda
type: CreativeWork
description: In 1963 the study of marine natural products was just beginning and there were only a handful of public records with only one of these publications reporting a new compound. There has since been 9220 papers published, reporting on 24 662 new compounds. Annually a variety of cembrane diterpenoids with a range of biological activities are reportedly isolated from marine soft coral, and most notably these compounds possess anticancer properties. The investigation of a marine soft coral, Sinularia notanda, for novel bioactive compounds is presented here. The aim was to identify compounds active against key HIV enzymes as well as against cervical cancer, an opportunistic malignancy affecting many HIV positive women in Sub-Saharan Africa. A methanol extract of S. notanda was prepared and the cytotoxicity of the crude extract tested against a cervical cancer (HeLa) cell line using sodium 2,3-bis-(2-methoxy-4-nitro- 5-sulfophenyl)-2H-tetrazolium- 5-carboxanilide (XTT). The crude extract was also tested for HIV-1 protease inhibition using a direct enzyme assay, and antioxidant activity determined using 1,1-diphenyl-2picrylhydrazyl (DPPH). Bioassay guided fractionation, column chromatography and thin layer chromatography were used to identify active fractions from the total extract as well as to isolate biologically active compounds. The isolated compounds were tested for cytotoxic activity against four different cell lines. Nuclear magnetic resonance spectroscopy and x-ray crystallography were used for structure determination. The crude ethyl acetate fraction of S. notanda had a 50% cytotoxic concentration (CC50) of 33.82 ?g/ml, exhibited moderate inhibition of HIV-1 protease (between 40 and 60% inhibition), was unable to inhibit reverse transcriptase and showed antioxidant activity at a concentration (IC50) of 76.15 ?g/ml. In comparison, the crude methanol fraction had a CC50 of 145.80 ?g/ml, showed significantly lower protease inhibition (p < 0.05), and showed antioxidant …
abstract: In 1963 the study of marine natural products was just beginning and there were only a handful of public records with only one of these publications reporting a new compound. There has since been 9220 papers published, reporting on 24 662 new compounds. Annually a variety of cembrane diterpenoids with a range of biological activities are reportedly isolated from marine soft coral, and most notably these compounds possess anticancer properties. The investigation of a marine soft coral, Sinularia notanda, for novel bioactive compounds is presented here. The aim was to identify compounds active against key HIV enzymes as well as against cervical cancer, an opportunistic malignancy affecting many HIV positive women in Sub-Saharan Africa. A methanol extract of S. notanda was prepared and the cytotoxicity of the crude extract tested against a cervical cancer (HeLa) cell line using sodium 2,3-bis-(2-methoxy-4-nitro- 5-sulfophenyl)-2H-tetrazolium- 5-carboxanilide (XTT). The crude extract was also tested for HIV-1 protease inhibition using a direct enzyme assay, and antioxidant activity determined using 1,1-diphenyl-2picrylhydrazyl (DPPH). Bioassay guided fractionation, column chromatography and thin layer chromatography were used to identify active fractions from the total extract as well as to isolate biologically active compounds. The isolated compounds were tested for cytotoxic activity against four different cell lines. Nuclear magnetic resonance spectroscopy and x-ray crystallography were used for structure determination. The crude ethyl acetate fraction of S. notanda had a 50% cytotoxic concentration (CC50) of 33.82 ?g/ml, exhibited moderate inhibition of HIV-1 protease (between 40 and 60% inhibition), was unable to inhibit reverse transcriptase and showed antioxidant activity at a concentration (IC50) of 76.15 ?g/ml. In comparison, the crude methanol fraction had a CC50 of 145.80 ?g/ml, showed significantly lower protease inhibition (p < 0.05), and showed antioxidant …
language: en
keywords: UCTD
date published: 2015
date modified: 2026-07-24T03:52:21Z
provider: University of Pretoria
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Pretoria

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2263/53497