An investigation into the antineoplastic potential of Bridelia Micrantha Constituents
type: CreativeWork
description: The fight against cancer is on going. This study provides a brief overview of the current status of cancer. Natural products as a source of clinically relevant, biologically active compounds, is the central theme of this study. Special reference has been made to antineoplastic (cancer-combating) compounds derived from botanicals. Bridelia micrantha is a multipurpose tree used crudely by several African cultures for the treatment of a variety of ailments. Suggestive cytotoxic results attained from a preliminary toxicological screening along with the knowledge that the genus Bridelia has proven to be a source of novel cytotoxic aryltetralin lignan glycosides (structurally related to podophyllotoxin), served as motivation for undertaking this study. The in vitro antineoplastic potential of several different solvent extracts of B. micrantha (bark) has been investigated. Five out of the six primary extracts displayed impressive cytotoxicity (IC50<10µg/ml) towards three out of the four tested neoplastic cell lines. Indicative of good antineoplastic potential, these same extracts did not inhibit the growth of primary porcine hepatocytes and furthermore significantly (P<0.05) stimulated the proliferation of primary human lymphocytes at comparable, physiologically relevant concentrations. The presence of numerous structurally distinct cytotoxic compounds was shown by statistical comparisons (2way ANOVA and Bonferroni multiple comparison post-tests) of the IC50< values produced by each of the primary extracts on each of the tested neoplastic cell cultures. The manner of cell death induction and points of cell cycle inhibition induced by the most promising of the primary extracts (H2<0SIS< - lyophilised aqueous extract, soluble in EtOH) at a fixed concentration of 10ìg/ml, was assessed flow cytometrically using COLO 320DM (human colorectal adenocarcinoma) cells. After 24hours incubation, extract H20S1S was shown to induce significant apoptosis (P<0.005) relative to appropriate…
abstract: The fight against cancer is on going. This study provides a brief overview of the current status of cancer. Natural products as a source of clinically relevant, biologically active compounds, is the central theme of this study. Special reference has been made to antineoplastic (cancer-combating) compounds derived from botanicals. Bridelia micrantha is a multipurpose tree used crudely by several African cultures for the treatment of a variety of ailments. Suggestive cytotoxic results attained from a preliminary toxicological screening along with the knowledge that the genus Bridelia has proven to be a source of novel cytotoxic aryltetralin lignan glycosides (structurally related to podophyllotoxin), served as motivation for undertaking this study. The in vitro antineoplastic potential of several different solvent extracts of B. micrantha (bark) has been investigated. Five out of the six primary extracts displayed impressive cytotoxicity (IC50<10µg/ml) towards three out of the four tested neoplastic cell lines. Indicative of good antineoplastic potential, these same extracts did not inhibit the growth of primary porcine hepatocytes and furthermore significantly (P<0.05) stimulated the proliferation of primary human lymphocytes at comparable, physiologically relevant concentrations. The presence of numerous structurally distinct cytotoxic compounds was shown by statistical comparisons (2way ANOVA and Bonferroni multiple comparison post-tests) of the IC50< values produced by each of the primary extracts on each of the tested neoplastic cell cultures. The manner of cell death induction and points of cell cycle inhibition induced by the most promising of the primary extracts (H2<0SIS< - lyophilised aqueous extract, soluble in EtOH) at a fixed concentration of 10ìg/ml, was assessed flow cytometrically using COLO 320DM (human colorectal adenocarcinoma) cells. After 24hours incubation, extract H20S1S was shown to induce significant apoptosis (P<0.005) relative to appropriate…
language: en
keywords: UCTD, Antineoplastic, Bioassay-guided fractionation, Botanicals, Bridelia micrantha, Cancer, Chemotaxonomy, Ethnobotanical, Phytochemical, Secondary plant metabolites, Chromatography
date published: 2007-09-25
date modified: 2026-07-24T03:52:32Z
provider: University of Pretoria
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Pretoria

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2263/31302