Elastase inhibitory, anti-inflammatory, and antioxidant potential of South African plants in a multi-faceted anti-ageing approach
type: CreativeWork
description: Ageing is a complex biological process constituting states of oxidative stress and chronic inflammation that result in the abnormal degradation of the structural components within the dermis. These components, namely elastin and collagen are vital in conferring integrity to the structure of the skin. Oxidative stress due to hydrogen peroxide and superoxide, and chronic inflammation due to the action of cyclooxygenase – II and 5-lipoxygenase result in the release of proteases such as elastase which hydrolyze the structural components if the dermis undesirably. This results in the formation of wrinkles. In the present study eleven terrestrial and wetland plants (Aloe ferox, Aloe spicata, Annona senegalensis, Barleria albostellata, Bulbine frutescens, Bulbine latifolia, Commelina benghalensis, Elephantorrhiza elephantina, Equisetum ramosissimum, Persicaria senegalensis and Plantago longissima) were investigated based on traditional reports of their use in skin care for the treatment of eczema, psoriasis and wound healing, in which elastase has been implicated. Water and ethanolic extracts were prepared for each plant based on their relevance to traditional means of preparation and industry standards. The ability of Streptococcus mutans (ATCC 25175) to enhance the elastase inhibitory potential of these extracts through biotransformation was investigated using liquidstate fermentation. Bioautography revealed minor zones of inhibition for P. senegalensis (water), B. latifolia (water and ethanol), A. senegalensis (bark and twigs, ethanol), A. ferox (ethanol), C. benghalensis (ethanol), B. albostellata (ethanol and water), B. latifolia and B. frutescens. Despite this, the fermentation was considered successful as all extracts exhibited visual differences in the chemical profile of fermented and non-fermented counterparts when observed using thin layer chromatography. The elastase inhibitory potential of the extracts was investigated by measuring the binding affinity of porc…
abstract: Ageing is a complex biological process constituting states of oxidative stress and chronic inflammation that result in the abnormal degradation of the structural components within the dermis. These components, namely elastin and collagen are vital in conferring integrity to the structure of the skin. Oxidative stress due to hydrogen peroxide and superoxide, and chronic inflammation due to the action of cyclooxygenase – II and 5-lipoxygenase result in the release of proteases such as elastase which hydrolyze the structural components if the dermis undesirably. This results in the formation of wrinkles. In the present study eleven terrestrial and wetland plants (Aloe ferox, Aloe spicata, Annona senegalensis, Barleria albostellata, Bulbine frutescens, Bulbine latifolia, Commelina benghalensis, Elephantorrhiza elephantina, Equisetum ramosissimum, Persicaria senegalensis and Plantago longissima) were investigated based on traditional reports of their use in skin care for the treatment of eczema, psoriasis and wound healing, in which elastase has been implicated. Water and ethanolic extracts were prepared for each plant based on their relevance to traditional means of preparation and industry standards. The ability of Streptococcus mutans (ATCC 25175) to enhance the elastase inhibitory potential of these extracts through biotransformation was investigated using liquidstate fermentation. Bioautography revealed minor zones of inhibition for P. senegalensis (water), B. latifolia (water and ethanol), A. senegalensis (bark and twigs, ethanol), A. ferox (ethanol), C. benghalensis (ethanol), B. albostellata (ethanol and water), B. latifolia and B. frutescens. Despite this, the fermentation was considered successful as all extracts exhibited visual differences in the chemical profile of fermented and non-fermented counterparts when observed using thin layer chromatography. The elastase inhibitory potential of the extracts was investigated by measuring the binding affinity of porc…
language: en
keywords: Elastase, Cytotoxicity, Free radicals, Fermentation, Inflammation
date published: 2018
date modified: 2026-07-24T03:52:23Z
provider: University of Pretoria
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Pretoria

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2263/110090