{"id":{"repo_id":"plymouth","oai_identifier":"oai:pearl.plymouth.ac.uk:foh-theses-other-1042"},"canonical_url":"https://search.dev.ndltd.org/etd/plymouth/oai:pearl.plymouth.ac.uk:foh-theses-other-1042","repository":{"repo_id":"plymouth","name":"University of Plymouth","base_url":"https://pearl.plymouth.ac.uk/do/oai"},"display":{"title":"An investigation of T cell dysregulation in B-cell chronic lymphocytic leukaemia","abstract":"B-cell chronic lymphocytic leukaemia (B-CLL) is characterized by an accumulation of clonal malignant B cells within lymphoid tissue, the bone marrow and the peripheral blood. Whilst abnormalities of these B cells are the essential cause of this disease, the aim of this research project was to investigate whether the T cell compartment may play a role in the aetiology of this disease by evaluating the expression of key surface antigens involved in both activation of and interaction with B cells and other antigen presenting cells of the immune system. There were marked abnormalities in the expression of certain key activation and interaction antigens on the peripheral blood T cells of patients with B-CLL, in particular, compared to normal controls, there was a significant reduction in the number of circulating T cells expressing CD25, CD28, CD152, CD4, CD5 and CD11a. There was no difference in expression of TCRαβ, CD8, CD54 and CD154. Significantly more T cells from CLL patients expressed HLA-DR. Removal of the malignant clone of cells prior to short-term T cell culture did not affect expression of these markers. Numbers of T cells expressing intracellular CD25 and CD152 were not decreased after activation and a significantly greater number of resting T cells expressed both antigens intracellularly. There was also evidence of a soluble factor present in CLL AB serum which caused increased numbers of normal and CLL T cells to express CD25 and CD152 after culture. Initial results suggest that this may be IFN-γ, levels of which were significantly higher, as measured by ELISA, from resting CLL T cells compared to normals. By studying the expression of these antigens using cell culture, flow cytometric and ELISA techniques, the results suggest a functional state of anergy in these T cells. This anergic state may contribute to the pathogenesis of B-CLL and its related phenomena of immunosuppression and autoimmunity. This was further reflected in the results of the T cell functional studies and reduced IL-2 expression in the mixed lymphocyte reaction (MLR).","abstract_html":"B-cell chronic lymphocytic leukaemia (B-CLL) is characterized by an accumulation of clonal malignant B cells within lymphoid tissue, the bone marrow and the peripheral blood. Whilst abnormalities of these B cells are the essential cause of this disease, the aim of this research project was to investigate whether the T cell compartment may play a role in the aetiology of this disease by evaluating the expression of key surface antigens involved in both activation of and interaction with B cells and other antigen presenting cells of the immune system. There were marked abnormalities in the expression of certain key activation and interaction antigens on the peripheral blood T cells of patients with B-CLL, in particular, compared to normal controls, there was a significant reduction in the number of circulating T cells expressing CD25, CD28, CD152, CD4, CD5 and CD11a. There was no difference in expression of TCRαβ, CD8, CD54 and CD154. Significantly more T cells from CLL patients expressed HLA-DR. Removal of the malignant clone of cells prior to short-term T cell culture did not affect expression of these markers. Numbers of T cells expressing intracellular CD25 and CD152 were not decreased after activation and a significantly greater number of resting T cells expressed both antigens intracellularly. There was also evidence of a soluble factor present in CLL AB serum which caused increased numbers of normal and CLL T cells to express CD25 and CD152 after culture. Initial results suggest that this may be IFN-γ, levels of which were significantly higher, as measured by ELISA, from resting CLL T cells compared to normals. By studying the expression of these antigens using cell culture, flow cytometric and ELISA techniques, the results suggest a functional state of anergy in these T cells. This anergic state may contribute to the pathogenesis of B-CLL and its related phenomena of immunosuppression and autoimmunity. This was further reflected in the results of the T cell functional studies and reduced IL-2 expression in the mixed lymphocyte reaction (MLR).","abstract_has_math":false,"creators":["SCRIVENER, SOPHIE GRACE"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2003,"date_issued":"2003-01-01T08:00:00Z","date_published":"2003-01-01T08:00:00Z","updated_at":"2026-07-24T03:50:23Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["10026.1/2735"],"render_values":[{"text":"10026.1/2735","href":null,"code":true}]}]},"links":{"outbound_url":"https://pearl.plymouth.ac.uk/foh-theses-other/42","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["SCRIVENER, SOPHIE GRACE"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2003-01-01T08:00:00Z"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["10026.1/2735","https://pearl.plymouth.ac.uk/foh-theses-other/42"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["B-cell chronic lymphocytic leukaemia (B-CLL) is characterized by an accumulation of clonal malignant B cells within lymphoid tissue, the bone marrow and the peripheral blood. Whilst abnormalities of these B cells are the essential cause of this disease, the aim of this research project was to investigate whether the T cell compartment may play a role in the aetiology of this disease by evaluating the expression of key surface antigens involved in both activation of and interaction with B cells and other antigen presenting cells of the immune system. There were marked abnormalities in the expression of certain key activation and interaction antigens on the peripheral blood T cells of patients with B-CLL, in particular, compared to normal controls, there was a significant reduction in the number of circulating T cells expressing CD25, CD28, CD152, CD4, CD5 and CD11a. There was no difference in expression of TCRαβ, CD8, CD54 and CD154. Significantly more T cells from CLL patients expressed HLA-DR. Removal of the malignant clone of cells prior to short-term T cell culture did not affect expression of these markers. Numbers of T cells expressing intracellular CD25 and CD152 were not decreased after activation and a significantly greater number of resting T cells expressed both antigens intracellularly. There was also evidence of a soluble factor present in CLL AB serum which caused increased numbers of normal and CLL T cells to express CD25 and CD152 after culture. Initial results suggest that this may be IFN-γ, levels of which were significantly higher, as measured by ELISA, from resting CLL T cells compared to normals. By studying the expression of these antigens using cell culture, flow cytometric and ELISA techniques, the results suggest a functional state of anergy in these T cells. This anergic state may contribute to the pathogenesis of B-CLL and its related phenomena of immunosuppression and autoimmunity. This was further reflected in the results of the T cell functional studies and reduced IL-2 expression in the mixed lymphocyte reaction (MLR)."]},{"key":"dc:title","label":"Title","values":["An investigation of T cell dysregulation in B-cell chronic lymphocytic leukaemia"]}]}],"canonical_facts":{"dc:creator":["SCRIVENER, SOPHIE GRACE"],"dc:date.issued":["2003-01-01T08:00:00Z"],"dc:description.abstract":["B-cell chronic lymphocytic leukaemia (B-CLL) is characterized by an accumulation of clonal malignant B cells within lymphoid tissue, the bone marrow and the peripheral blood. Whilst abnormalities of these B cells are the essential cause of this disease, the aim of this research project was to investigate whether the T cell compartment may play a role in the aetiology of this disease by evaluating the expression of key surface antigens involved in both activation of and interaction with B cells and other antigen presenting cells of the immune system. There were marked abnormalities in the expression of certain key activation and interaction antigens on the peripheral blood T cells of patients with B-CLL, in particular, compared to normal controls, there was a significant reduction in the number of circulating T cells expressing CD25, CD28, CD152, CD4, CD5 and CD11a. There was no difference in expression of TCRαβ, CD8, CD54 and CD154. Significantly more T cells from CLL patients expressed HLA-DR. Removal of the malignant clone of cells prior to short-term T cell culture did not affect expression of these markers. Numbers of T cells expressing intracellular CD25 and CD152 were not decreased after activation and a significantly greater number of resting T cells expressed both antigens intracellularly. There was also evidence of a soluble factor present in CLL AB serum which caused increased numbers of normal and CLL T cells to express CD25 and CD152 after culture. Initial results suggest that this may be IFN-γ, levels of which were significantly higher, as measured by ELISA, from resting CLL T cells compared to normals. By studying the expression of these antigens using cell culture, flow cytometric and ELISA techniques, the results suggest a functional state of anergy in these T cells. This anergic state may contribute to the pathogenesis of B-CLL and its related phenomena of immunosuppression and autoimmunity. This was further reflected in the results of the T cell functional studies and reduced IL-2 expression in the mixed lymphocyte reaction (MLR)."],"dc:identifier":["10026.1/2735","https://pearl.plymouth.ac.uk/foh-theses-other/42"],"dc:language":["eng"],"dc:title":["An investigation of T cell dysregulation in B-cell chronic lymphocytic leukaemia"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T03:50:23Z"}