{"id":{"repo_id":"penn","oai_identifier":"oai:repository.upenn.edu:20.500.14332/31131"},"canonical_url":"https://search.dev.ndltd.org/etd/penn/oai:repository.upenn.edu:20.500.14332/31131","repository":{"repo_id":"penn","name":"University of Pennsylvania","base_url":"https://repository.upenn.edu/server/oai/request"},"display":{"title":"T-Bet Expressing B Cells With Distinct Residency And Functional Characteristics Give Rise To Plasma Cells","abstract":"An increasing body of work shows that T-bet+ B cells play important roles in immune responses to infections a well as in autoimmune diseases. These studies investigate the kinetics, tissue distribution, and functions of T-bet+ B cells in a murine influenza infection model. Both T-bet+ and T-bet- HA-specific B cells emerge early after infection, acquire the memory markers CD73, PD-L2 and CD80, and persist indefinitely with limited interconversion between T-bet+ and T-bet- B cell pools. Moreover, T-bet+ B cells are required for HA stalk specific antibodies and sustained protective HAI titers. T-bet+ HA-specific B cells can be further divided into T-bethigh and T-betlow B cell pools. While T-bethigh, T-betlow, and T-bet- HA-specific B cells are initially found in the spleen, lungs, and draining mediastinal lymph nodes; at later timepoints T-bethigh HA-specific memory B cells are restricted to the spleen, despite the continued presence of T-bet- HA-specific B cells at other anatomical locations. Parabiotic studies show that HA-specific T-bethigh B cells are splenic residents, whereas T-betlow and T-bet- B cells recirculate. T-bethigh B cells give rise to T-betlow B cells but T-betlow B cells do not become T-bethigh. However, T-betlow B cells persist for a minimum of three weeks in the absence of T-bethigh B cells, indicating that they are likely distinct populations. CD138 levels increase with decreasing T-bet expression and T-betlow and T-bethigh B cells downregulate T-bet and give rise to plasma cells. Both B220+ and B220- plasma cells arise from T-bet+ B cells in the spleen and bone marrow. Together, these data suggest that T-bethigh memory B cells are a unique splenic resident population with stem cell like properties that sustains plasma cell numbers and protective antibody titers long term.","abstract_html":"An increasing body of work shows that T-bet+ B cells play important roles in immune responses to infections a well as in autoimmune diseases. These studies investigate the kinetics, tissue distribution, and functions of T-bet+ B cells in a murine influenza infection model. Both T-bet+ and T-bet- HA-specific B cells emerge early after infection, acquire the memory markers CD73, PD-L2 and CD80, and persist indefinitely with limited interconversion between T-bet+ and T-bet- B cell pools. Moreover, T-bet+ B cells are required for HA stalk specific antibodies and sustained protective HAI titers. T-bet+ HA-specific B cells can be further divided into T-bethigh and T-betlow B cell pools. While T-bethigh, T-betlow, and T-bet- HA-specific B cells are initially found in the spleen, lungs, and draining mediastinal lymph nodes; at later timepoints T-bethigh HA-specific memory B cells are restricted to the spleen, despite the continued presence of T-bet- HA-specific B cells at other anatomical locations. Parabiotic studies show that HA-specific T-bethigh B cells are splenic residents, whereas T-betlow and T-bet- B cells recirculate. T-bethigh B cells give rise to T-betlow B cells but T-betlow B cells do not become T-bethigh. However, T-betlow B cells persist for a minimum of three weeks in the absence of T-bethigh B cells, indicating that they are likely distinct populations. CD138 levels increase with decreasing T-bet expression and T-betlow and T-bethigh B cells downregulate T-bet and give rise to plasma cells. Both B220+ and B220- plasma cells arise from T-bet+ B cells in the spleen and bone marrow. Together, these data suggest that T-bethigh memory B cells are a unique splenic resident population with stem cell like properties that sustains plasma cell numbers and protective antibody titers long term.","abstract_has_math":false,"creators":["Rosenthal, Rebecca Laura"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Michael P. Cancro"],"committee_chairs":[],"committee_members":[],"year":2020,"date_issued":"2020","date_published":"2020","updated_at":"2026-07-24T03:46:32Z","subjects":[],"languages":["en"],"rights":["Rebecca Laura Rosenthal"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://repository.upenn.edu/handle/20.500.14332/31131","outbound_label":"Repository record","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Michael P. 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These studies investigate the kinetics, tissue distribution, and functions of T-bet+ B cells in a murine influenza infection model. Both T-bet+ and T-bet- HA-specific B cells emerge early after infection, acquire the memory markers CD73, PD-L2 and CD80, and persist indefinitely with limited interconversion between T-bet+ and T-bet- B cell pools. Moreover, T-bet+ B cells are required for HA stalk specific antibodies and sustained protective HAI titers. T-bet+ HA-specific B cells can be further divided into T-bethigh and T-betlow B cell pools. While T-bethigh, T-betlow, and T-bet- HA-specific B cells are initially found in the spleen, lungs, and draining mediastinal lymph nodes; at later timepoints T-bethigh HA-specific memory B cells are restricted to the spleen, despite the continued presence of T-bet- HA-specific B cells at other anatomical locations. Parabiotic studies show that HA-specific T-bethigh B cells are splenic residents, whereas T-betlow and T-bet- B cells recirculate. T-bethigh B cells give rise to T-betlow B cells but T-betlow B cells do not become T-bethigh. However, T-betlow B cells persist for a minimum of three weeks in the absence of T-bethigh B cells, indicating that they are likely distinct populations. CD138 levels increase with decreasing T-bet expression and T-betlow and T-bethigh B cells downregulate T-bet and give rise to plasma cells. Both B220+ and B220- plasma cells arise from T-bet+ B cells in the spleen and bone marrow. Together, these data suggest that T-bethigh memory B cells are a unique splenic resident population with stem cell like properties that sustains plasma cell numbers and protective antibody titers long term."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Doctor of Philosophy (PhD)"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["T-Bet Expressing B Cells With Distinct Residency And Functional Characteristics Give Rise To Plasma Cells"]}]}],"canonical_facts":{"dc:contributor.advisor":["Michael P. Cancro"],"dc:creator":["Rosenthal, Rebecca Laura"],"dc:date":["2023-05-18T01:16:18.000"],"dc:date.accessioned":["2023-05-22T18:01:17Z"],"dc:date.available":["2001-01-01T00:00:00Z"],"dc:date.issued":["2020"],"dc:description.abstract":["An increasing body of work shows that T-bet+ B cells play important roles in immune responses to infections a well as in autoimmune diseases. These studies investigate the kinetics, tissue distribution, and functions of T-bet+ B cells in a murine influenza infection model. Both T-bet+ and T-bet- HA-specific B cells emerge early after infection, acquire the memory markers CD73, PD-L2 and CD80, and persist indefinitely with limited interconversion between T-bet+ and T-bet- B cell pools. Moreover, T-bet+ B cells are required for HA stalk specific antibodies and sustained protective HAI titers. T-bet+ HA-specific B cells can be further divided into T-bethigh and T-betlow B cell pools. While T-bethigh, T-betlow, and T-bet- HA-specific B cells are initially found in the spleen, lungs, and draining mediastinal lymph nodes; at later timepoints T-bethigh HA-specific memory B cells are restricted to the spleen, despite the continued presence of T-bet- HA-specific B cells at other anatomical locations. Parabiotic studies show that HA-specific T-bethigh B cells are splenic residents, whereas T-betlow and T-bet- B cells recirculate. T-bethigh B cells give rise to T-betlow B cells but T-betlow B cells do not become T-bethigh. However, T-betlow B cells persist for a minimum of three weeks in the absence of T-bethigh B cells, indicating that they are likely distinct populations. CD138 levels increase with decreasing T-bet expression and T-betlow and T-bethigh B cells downregulate T-bet and give rise to plasma cells. Both B220+ and B220- plasma cells arise from T-bet+ B cells in the spleen and bone marrow. Together, these data suggest that T-bethigh memory B cells are a unique splenic resident population with stem cell like properties that sustains plasma cell numbers and protective antibody titers long term."],"dc:description.degree":["Doctor of Philosophy (PhD)"],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["https://repository.upenn.edu/handle/20.500.14332/31131"],"dc:language":["en"],"dc:rights":["Rebecca Laura Rosenthal"],"dc:title":["T-Bet Expressing B Cells With Distinct Residency And Functional Characteristics Give Rise To Plasma Cells"],"dc:type":["Dissertation/Thesis"]},"updated_at":"2026-07-24T03:46:32Z"}