Development of analytical methodology based on chromatographic and mass spectrometric techniques for the characterization and fingerprinting of bioactive compounds of Crocus sativus L.
type: CreativeWork
description: The present research study included the development of analytical methodology based on chromatographic and mass spectrometric techniques for the determination and fingerprinting of bioactive compounds derived from Crocus sativus L. and also their interaction with biomolecules, with the aim to investigate their role in human health. The main bioactive analyzed compounds were picrocrocin, crocetin and analogues thereof, which are unusually glucosylated carotenoids (ranging from one up to a total five glucosyl-residues) and specifically Gentiobiosyl-gentiotriosyl-E-crocetin (trans-crocin-5, TC-5), Gentiobiosyl-neapolitanosyl-E-crocetin (trans-crocin-5′, TC-5′) bis-Gentiobiosyl-E-crocetin (trans-crocin-4, TC-4), Gentiobiosyl-glucosyl-E-crocetin (trans-crocin-3, TC-3), bis-Glucosyl-E-crocetin (trans-crocin-2, TC-2), (4) bis-Gentiobiosyl-13-Z-crocetin (cis-crocin-4, CC-4), Gentiobiosyl-glucosyl-13-Z-crocetin (cis-crocin-3, CC-3), Crocetin (CRT) and Picrocrocin (PICR) with ultimate objective the assessment of their biological effect on the prevention and treatment of various neurodegenerative diseases like Parkinson's and Alzheimer's disease. Because of their particular complicated structural motif and the extendeed degree of symmetry which constitutes a rather complex mixture of glycoconjugates it became imperative to isolate and characterize their structures mainly with state-of-the-art chromatographic and mass spectrometric techniques. The developed methodologies included the use of the following modern analytical techniques and instrumentation: i) Size-exclusion chromatography (SEC), Medium pressure liquid chromatography (MPLC), semi-preparative High Performance Liquid Chromatography (semi-Prep HPLC) and Fast Centrifugation Partition Chromatography (FCPC) for the separation and isolation of aforementioned bioactive compounds, ii) various tandem mass spectrometric techniques such as MALDI-TOF/RTOF-MS in combination with collision-induced dissociation, ESI-QqRTOF-MS/MS, …
abstract: The present research study included the development of analytical methodology based on chromatographic and mass spectrometric techniques for the determination and fingerprinting of bioactive compounds derived from Crocus sativus L. and also their interaction with biomolecules, with the aim to investigate their role in human health. The main bioactive analyzed compounds were picrocrocin, crocetin and analogues thereof, which are unusually glucosylated carotenoids (ranging from one up to a total five glucosyl-residues) and specifically Gentiobiosyl-gentiotriosyl-E-crocetin (trans-crocin-5, TC-5), Gentiobiosyl-neapolitanosyl-E-crocetin (trans-crocin-5′, TC-5′) bis-Gentiobiosyl-E-crocetin (trans-crocin-4, TC-4), Gentiobiosyl-glucosyl-E-crocetin (trans-crocin-3, TC-3), bis-Glucosyl-E-crocetin (trans-crocin-2, TC-2), (4) bis-Gentiobiosyl-13-Z-crocetin (cis-crocin-4, CC-4), Gentiobiosyl-glucosyl-13-Z-crocetin (cis-crocin-3, CC-3), Crocetin (CRT) and Picrocrocin (PICR) with ultimate objective the assessment of their biological effect on the prevention and treatment of various neurodegenerative diseases like Parkinson's and Alzheimer's disease. Because of their particular complicated structural motif and the extendeed degree of symmetry which constitutes a rather complex mixture of glycoconjugates it became imperative to isolate and characterize their structures mainly with state-of-the-art chromatographic and mass spectrometric techniques. The developed methodologies included the use of the following modern analytical techniques and instrumentation: i) Size-exclusion chromatography (SEC), Medium pressure liquid chromatography (MPLC), semi-preparative High Performance Liquid Chromatography (semi-Prep HPLC) and Fast Centrifugation Partition Chromatography (FCPC) for the separation and isolation of aforementioned bioactive compounds, ii) various tandem mass spectrometric techniques such as MALDI-TOF/RTOF-MS in combination with collision-induced dissociation, ESI-QqRTOF-MS/MS, …
author: Κουλακιώτης, Νικόλαος Σταύρος
language: en
keywords: Crocus sativus L., Stigmas, Apocarotenoid ester glycosides, Reversed-phase HPLC, Quantification, Validation, Mass Spectrometry, Crocins, Picrocrocin, MALDI-MS, ESI-MS, Tandem mass spectrometry, High-energy CID, Low-energy CID, Multistage mass spectrometry, Accurate mass measurement, Noncovalent complex, Alzheimer's disease, Αmyloid‐β‐peptide, 615.324 38, Κρόκος Κοζάνης, Στίγματα, Σαφράν, Αποκαροτενοειδή, Κροκίνες, Πικροκροκίνη, Απομόνωση, Χρωματογραφία κατ’ αντιροή, Υγρή χρωματογραφία υψηλής απόδοσης, Επικύρωση μεθόδου, Ποσοτικοποίηση, Φασματομετρία μάζας, Δίδυμη φασματομετρία μάζας, Ιονισμός μέσω ηλεκτροψεκασμού, Αμυλοειδές πεπτίδιο, Νόσος Αλτσχάιμερ
date published: 2016-03-03T07:25:18Z
date modified: 2026-07-27T20:29:32Z
provider: University of Patras
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Patras

PropertyValue
property ID: Handle
value: http://hdl.handle.net/10889/9181