Metastatic cell responses to anticancer therapy: The possible role of cancer stem cells
type: CreativeWork
description: Metastasis is a multistep process, by which cancer cells dissociate from the initial site and travel to form new secondary tumours at distant sites. This process has been reported to require epithelial-mesenchymal transition (EMT), by which the epithelial cancer cells convert to a mesenchymal form, associated with increasing levels of vimentin and decrease in E-cadherin markers. Cancer metastasis is also associated with aberrations in cells’ glycosylation such as Tn antigen which can be detected by Helix pomatia agglutinin (HPA) lectin extract from Helix pomatia snails. Radiotherapy could have an effect on cancer metastasis; by stimulating regulation signalling including ROS, TGF-β, E-cadherin and vimentin. This signalling can promote cancer cells to change from the epithelial to mesenchymal form. The cells behaviour could be mediated by the production of extracellular vesicles/exosomes from irradiated cells, which may have an impact on unirradiated cells. To date, the role of direct and bystander effect of ionising irradiation on the cancer cells glycosylation, EMT markers and invasiveness has not yet been investigated. Therefore, a study has been established to determine the direct effect, and if the progeny of cancer cells that survive a therapeutic dose of X-radiation show differences in aberrant glycosylation associated with metastasis, EMT markers and their ability to invade compared to unirradiated cells. The study also aims to investigate the bystander effect of extracellular vesicle/exosomes in the invasion of breast cancer cells. The molecular mechanisms that could be involved in the invasion of breast cancer cells following irradiation or exosome transfer were also investigated. In this study, both cells directly exposed to a therapeutic dose of ionising radiation and unirradiated recipient cells treated with exosomes from irradiated cells showed an increase in vimentin immunopositivity, HPA positivity, a decrease in the E-cadherin immunopositivity and a …
abstract: Metastasis is a multistep process, by which cancer cells dissociate from the initial site and travel to form new secondary tumours at distant sites. This process has been reported to require epithelial-mesenchymal transition (EMT), by which the epithelial cancer cells convert to a mesenchymal form, associated with increasing levels of vimentin and decrease in E-cadherin markers. Cancer metastasis is also associated with aberrations in cells’ glycosylation such as Tn antigen which can be detected by Helix pomatia agglutinin (HPA) lectin extract from Helix pomatia snails. Radiotherapy could have an effect on cancer metastasis; by stimulating regulation signalling including ROS, TGF-β, E-cadherin and vimentin. This signalling can promote cancer cells to change from the epithelial to mesenchymal form. The cells behaviour could be mediated by the production of extracellular vesicles/exosomes from irradiated cells, which may have an impact on unirradiated cells. To date, the role of direct and bystander effect of ionising irradiation on the cancer cells glycosylation, EMT markers and invasiveness has not yet been investigated. Therefore, a study has been established to determine the direct effect, and if the progeny of cancer cells that survive a therapeutic dose of X-radiation show differences in aberrant glycosylation associated with metastasis, EMT markers and their ability to invade compared to unirradiated cells. The study also aims to investigate the bystander effect of extracellular vesicle/exosomes in the invasion of breast cancer cells. The molecular mechanisms that could be involved in the invasion of breast cancer cells following irradiation or exosome transfer were also investigated. In this study, both cells directly exposed to a therapeutic dose of ionising radiation and unirradiated recipient cells treated with exosomes from irradiated cells showed an increase in vimentin immunopositivity, HPA positivity, a decrease in the E-cadherin immunopositivity and a …
author: AL-Abedi, Raheem Abd Zaid Abed
language: en
date published: 2018
date modified: 2026-07-24T03:42:23Z
provider: Oxford Brookes University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Oxford Brookes University

PropertyValue
property ID: DOI
value: 10.24384/mqbw-1k74