{"id":{"repo_id":"ottawa-retro","oai_identifier":"oai:ruor.uottawa.ca:10393/9230"},"canonical_url":"https://search.dev.ndltd.org/etd/ottawa-retro/oai:ruor.uottawa.ca:10393/9230","repository":{"repo_id":"ottawa-retro","name":"University of Ottawa","base_url":"https://ruor.uottawa.ca/server/oai/request"},"display":{"title":"Short-term recovery from a volume increase in the mouse zygote: Characteristics of regulatory volume decrease in vitro.","abstract":"Mouse zygotes have been shown to recover from an imposed increase in volume, a process known as a regulatory volume decrease or RVD, via separate but functionally coupled Cl- and K+ channels. The objectives of this thesis were to further determine some of the characteristics of these Cl- and K+ channels involved in RVD in the mouse zygote. It was proposed that the Cl - channel in mouse zygotes was a swelling-activated Cl -/organic osmolyte channel like that found in many other cells. A method developed by Passantes-Morales et al. (1993) was used, in which a large extracellular concentration of one of a number of organic osmolytes was added to the experimental media, and the ability of the zygote to perform RVD in the presence of each was determined. A number of organic compounds demonstrated an inhibition of RVD, and thus, identified a number of organic compounds which might function as organic osmolytes in embryos. To directly test whether swelling of zygotes increased their permeability to organic osmolytes; the permeability in swelled zygotes was compared to that in non-swelled zygotes, using 3H glycine. (Abstract shortened by UMI.)","abstract_html":"Mouse zygotes have been shown to recover from an imposed increase in volume, a process known as a regulatory volume decrease or RVD, via separate but functionally coupled Cl- and K+ channels. The objectives of this thesis were to further determine some of the characteristics of these Cl- and K+ channels involved in RVD in the mouse zygote. It was proposed that the Cl - channel in mouse zygotes was a swelling-activated Cl -/organic osmolyte channel like that found in many other cells. A method developed by Passantes-Morales et al. (1993) was used, in which a large extracellular concentration of one of a number of organic osmolytes was added to the experimental media, and the ability of the zygote to perform RVD in the presence of each was determined. A number of organic compounds demonstrated an inhibition of RVD, and thus, identified a number of organic compounds which might function as organic osmolytes in embryos. To directly test whether swelling of zygotes increased their permeability to organic osmolytes; the permeability in swelled zygotes was compared to that in non-swelled zygotes, using 3H glycine. 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The objectives of this thesis were to further determine some of the characteristics of these Cl- and K+ channels involved in RVD in the mouse zygote. It was proposed that the Cl - channel in mouse zygotes was a swelling-activated Cl -/organic osmolyte channel like that found in many other cells. A method developed by Passantes-Morales et al. (1993) was used, in which a large extracellular concentration of one of a number of organic osmolytes was added to the experimental media, and the ability of the zygote to perform RVD in the presence of each was determined. A number of organic compounds demonstrated an inhibition of RVD, and thus, identified a number of organic compounds which might function as organic osmolytes in embryos. To directly test whether swelling of zygotes increased their permeability to organic osmolytes; the permeability in swelled zygotes was compared to that in non-swelled zygotes, using 3H glycine. (Abstract shortened by UMI.)"]},{"key":"dc:format","label":"Dc Format","values":["119 p.","application/pdf"]},{"key":"dc:title","label":"Title","values":["Short-term recovery from a volume increase in the mouse zygote: Characteristics of regulatory volume decrease in vitro."]}]}],"canonical_facts":{"dc:contributor":["Baltz, Jay M.,"],"dc:creator":["Séguin, Diane G."],"dc:date":["2009-03-23T18:22:53Z","2000"],"dc:description":["Mouse zygotes have been shown to recover from an imposed increase in volume, a process known as a regulatory volume decrease or RVD, via separate but functionally coupled Cl- and K+ channels. The objectives of this thesis were to further determine some of the characteristics of these Cl- and K+ channels involved in RVD in the mouse zygote. It was proposed that the Cl - channel in mouse zygotes was a swelling-activated Cl -/organic osmolyte channel like that found in many other cells. A method developed by Passantes-Morales et al. (1993) was used, in which a large extracellular concentration of one of a number of organic osmolytes was added to the experimental media, and the ability of the zygote to perform RVD in the presence of each was determined. A number of organic compounds demonstrated an inhibition of RVD, and thus, identified a number of organic compounds which might function as organic osmolytes in embryos. To directly test whether swelling of zygotes increased their permeability to organic osmolytes; the permeability in swelled zygotes was compared to that in non-swelled zygotes, using 3H glycine. 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