{"id":{"repo_id":"ottawa-retro","oai_identifier":"oai:ruor.uottawa.ca:10393/8646"},"canonical_url":"https://search.dev.ndltd.org/etd/ottawa-retro/oai:ruor.uottawa.ca:10393/8646","repository":{"repo_id":"ottawa-retro","name":"University of Ottawa","base_url":"https://ruor.uottawa.ca/server/oai/request"},"display":{"title":"Insulin and TSH signal transduction pathways in human preadipocytes.","abstract":"Signaling through the rapamycin-sensitive mammalian target of rapamycin-p70 S6 kinase (p70 S6K) pathway was examined in human preadipocytes in primary culture. The inhibitory effect of rapamycin on insulin-induced differentiation of preadipose cell lines has been attributed to a blockade of clonal expansion. We demonstrate that rapamycin inhibits the differentiation of human preadipocytes in primary culture, even though these cells do not undergo clonal expansion. Glycerol-3-phosphate-dehydrogenase (GPDH) activity, an indicator of differentiation, was reduced in omental and subcutaneous preadipocytes to 17 +/- 10% (mean +/- 95% confidence limits, n = 10) of standard differentiation. Our data suggest rapamycin-sensitive pathways operate independently of clonal expansion. Our second objective addressed a novel route of p70 S6K activation. We have detected the presence of TSH receptor in human preadipocytes, and have shown it activates PKB and p70 S6K in a wortmannin-sensitive manner. A model depicting the convergence of insulin and TSH signaling in human preadipocytes is proposed.","abstract_html":"Signaling through the rapamycin-sensitive mammalian target of rapamycin-p70 S6 kinase (p70 S6K) pathway was examined in human preadipocytes in primary culture. The inhibitory effect of rapamycin on insulin-induced differentiation of preadipose cell lines has been attributed to a blockade of clonal expansion. We demonstrate that rapamycin inhibits the differentiation of human preadipocytes in primary culture, even though these cells do not undergo clonal expansion. Glycerol-3-phosphate-dehydrogenase (GPDH) activity, an indicator of differentiation, was reduced in omental and subcutaneous preadipocytes to 17 +/- 10% (mean +/- 95% confidence limits, n = 10) of standard differentiation. Our data suggest rapamycin-sensitive pathways operate independently of clonal expansion. Our second objective addressed a novel route of p70 S6K activation. We have detected the presence of TSH receptor in human preadipocytes, and have shown it activates PKB and p70 S6K in a wortmannin-sensitive manner. A model depicting the convergence of insulin and TSH signaling in human preadipocytes is proposed.","abstract_has_math":false,"creators":["Bell, Andrea Dorothy Lee."],"institution":"University of Ottawa (Canada)","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Sorisky, Alexander,"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2009,"date_issued":"2009-03-23T17:34:18Z","date_published":"2009-03-23T17:34:18Z","updated_at":"2026-07-24T03:39:27Z","subjects":["Biology, Cell."],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["Source: Masters Abstracts International, Volume: 39-04, page: 1077.","9780612570863","http://dx.doi.org/10.20381/ruor-15921"],"render_values":[{"text":"Source: Masters Abstracts International, Volume: 39-04, page: 1077.","href":null,"code":true},{"text":"9780612570863","href":null,"code":true},{"text":"http://dx.doi.org/10.20381/ruor-15921","href":"http://dx.doi.org/10.20381/ruor-15921","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10393/8646","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Sorisky, Alexander,"]},{"key":"dc:creator","label":"Author","values":["Bell, Andrea Dorothy Lee."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2009-03-23T17:34:18Z","2000"]},{"key":"dc:publisher","label":"Institution","values":["University of Ottawa (Canada)"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Cell."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["Source: Masters Abstracts International, Volume: 39-04, page: 1077.","9780612570863","http://hdl.handle.net/10393/8646","http://dx.doi.org/10.20381/ruor-15921"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Signaling through the rapamycin-sensitive mammalian target of rapamycin-p70 S6 kinase (p70 S6K) pathway was examined in human preadipocytes in primary culture. The inhibitory effect of rapamycin on insulin-induced differentiation of preadipose cell lines has been attributed to a blockade of clonal expansion. We demonstrate that rapamycin inhibits the differentiation of human preadipocytes in primary culture, even though these cells do not undergo clonal expansion. Glycerol-3-phosphate-dehydrogenase (GPDH) activity, an indicator of differentiation, was reduced in omental and subcutaneous preadipocytes to 17 +/- 10% (mean +/- 95% confidence limits, n = 10) of standard differentiation. Our data suggest rapamycin-sensitive pathways operate independently of clonal expansion. Our second objective addressed a novel route of p70 S6K activation. We have detected the presence of TSH receptor in human preadipocytes, and have shown it activates PKB and p70 S6K in a wortmannin-sensitive manner. A model depicting the convergence of insulin and TSH signaling in human preadipocytes is proposed."]},{"key":"dc:format","label":"Dc Format","values":["99 p.","application/pdf"]},{"key":"dc:title","label":"Title","values":["Insulin and TSH signal transduction pathways in human preadipocytes."]}]}],"canonical_facts":{"dc:contributor":["Sorisky, Alexander,"],"dc:creator":["Bell, Andrea Dorothy Lee."],"dc:date":["2009-03-23T17:34:18Z","2000"],"dc:description":["Signaling through the rapamycin-sensitive mammalian target of rapamycin-p70 S6 kinase (p70 S6K) pathway was examined in human preadipocytes in primary culture. The inhibitory effect of rapamycin on insulin-induced differentiation of preadipose cell lines has been attributed to a blockade of clonal expansion. We demonstrate that rapamycin inhibits the differentiation of human preadipocytes in primary culture, even though these cells do not undergo clonal expansion. Glycerol-3-phosphate-dehydrogenase (GPDH) activity, an indicator of differentiation, was reduced in omental and subcutaneous preadipocytes to 17 +/- 10% (mean +/- 95% confidence limits, n = 10) of standard differentiation. Our data suggest rapamycin-sensitive pathways operate independently of clonal expansion. Our second objective addressed a novel route of p70 S6K activation. We have detected the presence of TSH receptor in human preadipocytes, and have shown it activates PKB and p70 S6K in a wortmannin-sensitive manner. A model depicting the convergence of insulin and TSH signaling in human preadipocytes is proposed."],"dc:format":["99 p.","application/pdf"],"dc:identifier":["Source: Masters Abstracts International, Volume: 39-04, page: 1077.","9780612570863","http://hdl.handle.net/10393/8646","http://dx.doi.org/10.20381/ruor-15921"],"dc:publisher":["University of Ottawa (Canada)"],"dc:subject":["Biology, Cell."],"dc:title":["Insulin and TSH signal transduction pathways in human preadipocytes."],"dc:type":["Thesis"]},"updated_at":"2026-07-24T03:39:27Z"}