{"id":{"repo_id":"ottawa-retro","oai_identifier":"oai:ruor.uottawa.ca:10393/6361"},"canonical_url":"https://search.dev.ndltd.org/etd/ottawa-retro/oai:ruor.uottawa.ca:10393/6361","repository":{"repo_id":"ottawa-retro","name":"University of Ottawa","base_url":"https://ruor.uottawa.ca/server/oai/request"},"display":{"title":"Mechanisms of 17-beta-estradiol regulation of the proto-oncogene Bcl-2 in MCF-7 human breast cancer cells.","abstract":"In the present studies, the mechanisms of estrogen regulation of Bcl-2 were investigated by analyzing the expression of different Bcl-2 promoter-driven constructs stably transfected into MCF-7 cells. An approximately 1.7 kilobase (kb) sequence which directed estrogen-dependent regulation of Bcl-2 expression in these stable MCF-7 clones was identified. Another agent which may play a role in the regulation of Bcl-2 expression is the tumour suppressor gene, p53. We investigated the effects of various mutant p53 proteins and low levels of p53 on Bcl-2 expression in MCF-7 cells. Neither MCF-7/E6 cells expressing virtually undetectable levels of p53 nor MCF-7/173L cells expressing a DNA binding domain mutant p53, showed altered E2-mediated induction of Bcl-2 mRNA or protein levels. However, MCF-7 cells expressing a truncated mutant p53 protein (Delta291) resulted in a dramatic decrease in Bcl-2 protein levels, but not mRNA levels, upon E2 treatment. These results suggest that a p53 protein lacking a carboxy-terminus may cooperate with E2 post-transcriptionally to negatively regulate Bcl-2 levels in MCF-7 human breast cancer cells. (Abstract shortened by UMI.)","abstract_html":"In the present studies, the mechanisms of estrogen regulation of Bcl-2 were investigated by analyzing the expression of different Bcl-2 promoter-driven constructs stably transfected into MCF-7 cells. An approximately 1.7 kilobase (kb) sequence which directed estrogen-dependent regulation of Bcl-2 expression in these stable MCF-7 clones was identified. Another agent which may play a role in the regulation of Bcl-2 expression is the tumour suppressor gene, p53. We investigated the effects of various mutant p53 proteins and low levels of p53 on Bcl-2 expression in MCF-7 cells. Neither MCF-7/E6 cells expressing virtually undetectable levels of p53 nor MCF-7/173L cells expressing a DNA binding domain mutant p53, showed altered E2-mediated induction of Bcl-2 mRNA or protein levels. However, MCF-7 cells expressing a truncated mutant p53 protein (Delta291) resulted in a dramatic decrease in Bcl-2 protein levels, but not mRNA levels, upon E2 treatment. These results suggest that a p53 protein lacking a carboxy-terminus may cooperate with E2 post-transcriptionally to negatively regulate Bcl-2 levels in MCF-7 human breast cancer cells. (Abstract shortened by UMI.)","abstract_has_math":false,"creators":["Kushwaha, Neena."],"institution":"University of Ottawa (Canada)","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Pratt, Christine,"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2009,"date_issued":"2009-03-23T13:06:53Z","date_published":"2009-03-23T13:06:53Z","updated_at":"2026-07-24T03:39:21Z","subjects":["Biology, Molecular."],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["Source: Masters Abstracts International, Volume: 40-05, page: 1198.","9780612660663","http://dx.doi.org/10.20381/ruor-11230"],"render_values":[{"text":"Source: Masters Abstracts International, Volume: 40-05, page: 1198.","href":null,"code":true},{"text":"9780612660663","href":null,"code":true},{"text":"http://dx.doi.org/10.20381/ruor-11230","href":"http://dx.doi.org/10.20381/ruor-11230","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10393/6361","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Pratt, Christine,"]},{"key":"dc:creator","label":"Author","values":["Kushwaha, Neena."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2009-03-23T13:06:53Z","2002"]},{"key":"dc:publisher","label":"Institution","values":["University of Ottawa (Canada)"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["Source: Masters Abstracts International, Volume: 40-05, page: 1198.","9780612660663","http://hdl.handle.net/10393/6361","http://dx.doi.org/10.20381/ruor-11230"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["In the present studies, the mechanisms of estrogen regulation of Bcl-2 were investigated by analyzing the expression of different Bcl-2 promoter-driven constructs stably transfected into MCF-7 cells. An approximately 1.7 kilobase (kb) sequence which directed estrogen-dependent regulation of Bcl-2 expression in these stable MCF-7 clones was identified. Another agent which may play a role in the regulation of Bcl-2 expression is the tumour suppressor gene, p53. We investigated the effects of various mutant p53 proteins and low levels of p53 on Bcl-2 expression in MCF-7 cells. Neither MCF-7/E6 cells expressing virtually undetectable levels of p53 nor MCF-7/173L cells expressing a DNA binding domain mutant p53, showed altered E2-mediated induction of Bcl-2 mRNA or protein levels. However, MCF-7 cells expressing a truncated mutant p53 protein (Delta291) resulted in a dramatic decrease in Bcl-2 protein levels, but not mRNA levels, upon E2 treatment. These results suggest that a p53 protein lacking a carboxy-terminus may cooperate with E2 post-transcriptionally to negatively regulate Bcl-2 levels in MCF-7 human breast cancer cells. (Abstract shortened by UMI.)"]},{"key":"dc:format","label":"Dc Format","values":["99 p.","application/pdf"]},{"key":"dc:title","label":"Title","values":["Mechanisms of 17-beta-estradiol regulation of the proto-oncogene Bcl-2 in MCF-7 human breast cancer cells."]}]}],"canonical_facts":{"dc:contributor":["Pratt, Christine,"],"dc:creator":["Kushwaha, Neena."],"dc:date":["2009-03-23T13:06:53Z","2002"],"dc:description":["In the present studies, the mechanisms of estrogen regulation of Bcl-2 were investigated by analyzing the expression of different Bcl-2 promoter-driven constructs stably transfected into MCF-7 cells. An approximately 1.7 kilobase (kb) sequence which directed estrogen-dependent regulation of Bcl-2 expression in these stable MCF-7 clones was identified. Another agent which may play a role in the regulation of Bcl-2 expression is the tumour suppressor gene, p53. We investigated the effects of various mutant p53 proteins and low levels of p53 on Bcl-2 expression in MCF-7 cells. Neither MCF-7/E6 cells expressing virtually undetectable levels of p53 nor MCF-7/173L cells expressing a DNA binding domain mutant p53, showed altered E2-mediated induction of Bcl-2 mRNA or protein levels. However, MCF-7 cells expressing a truncated mutant p53 protein (Delta291) resulted in a dramatic decrease in Bcl-2 protein levels, but not mRNA levels, upon E2 treatment. These results suggest that a p53 protein lacking a carboxy-terminus may cooperate with E2 post-transcriptionally to negatively regulate Bcl-2 levels in MCF-7 human breast cancer cells. (Abstract shortened by UMI.)"],"dc:format":["99 p.","application/pdf"],"dc:identifier":["Source: Masters Abstracts International, Volume: 40-05, page: 1198.","9780612660663","http://hdl.handle.net/10393/6361","http://dx.doi.org/10.20381/ruor-11230"],"dc:publisher":["University of Ottawa (Canada)"],"dc:subject":["Biology, Molecular."],"dc:title":["Mechanisms of 17-beta-estradiol regulation of the proto-oncogene Bcl-2 in MCF-7 human breast cancer cells."],"dc:type":["Thesis"]},"updated_at":"2026-07-24T03:39:21Z"}