{"id":{"repo_id":"ottawa-retro","oai_identifier":"oai:ruor.uottawa.ca:10393/46392"},"canonical_url":"https://search.dev.ndltd.org/etd/ottawa-retro/oai:ruor.uottawa.ca:10393/46392","repository":{"repo_id":"ottawa-retro","name":"University of Ottawa","base_url":"https://ruor.uottawa.ca/server/oai/request"},"display":{"title":"Killer Cell Immunoglobulin-Like Receptors Delineate Distinct Phenotypes and Functions in Human γδ T Cells","abstract":"Human circulating γδ T cells are broadly classified into three subsets: Vδ1, Vδ2, and Vδ1/2neg populations, with the Vδ2 subset being the most abundant. In this research, I focus on the contribution and interplay of Natural Killer (NK) receptors to the γδ T cell phenotype and function, with emphasis on Killer-cell Immunoglobulin-like Receptors (KIRs), which are poorly studied in the field of γδ T cell biology. Because cytomegalovirus (CMV) is known to shape the αβ T cell and NK cell repertoires, I studied peripheral blood (PB) γδ T cells from both CMV-seronegative (CMV-) and CMV-seropositive (CMV+) healthy adult humans, using spectral flow cytometry. I found that CMV leaves a stable imprint in the γδ T cell repertoire and phenotype. CMV+ individuals have increased proportions of Vδ1 T cells within the total γδ T cell population. Moreover, γδ T cells from these individuals display increased proportions of cells expressing KIRs, which constitute a highly polymorphic family of receptors for Human Leukocyte Antigen (HLA) class I. These KIRs delineate a dichotomy in the phenotype and function of γδ T cells. KIR+γδ T cells exhibit characteristics akin to memory like T cells, displaying heightened effector potential, whereas KIR-γδ T cells resemble naïve T cells with comparatively weaker immune responses. Leveraging the assay for transposase-accessible chromatin with sequencing (ATACseq) technology, I show that KIR+γδ T cells have more open loci associated with effector functions than KIR-γδ T cells, which is consistent with the above results. These findings underscore a critical role for KIRs in γδ T cell immunity and hold implications for both our understanding of immune responses influenced by CMV and the potential of γδ T cells in cellular immunotherapy.","abstract_html":"Human circulating γδ T cells are broadly classified into three subsets: Vδ1, Vδ2, and Vδ1/2neg populations, with the Vδ2 subset being the most abundant. In this research, I focus on the contribution and interplay of Natural Killer (NK) receptors to the γδ T cell phenotype and function, with emphasis on Killer-cell Immunoglobulin-like Receptors (KIRs), which are poorly studied in the field of γδ T cell biology. Because cytomegalovirus (CMV) is known to shape the αβ T cell and NK cell repertoires, I studied peripheral blood (PB) γδ T cells from both CMV-seronegative (CMV-) and CMV-seropositive (CMV+) healthy adult humans, using spectral flow cytometry. I found that CMV leaves a stable imprint in the γδ T cell repertoire and phenotype. CMV+ individuals have increased proportions of Vδ1 T cells within the total γδ T cell population. Moreover, γδ T cells from these individuals display increased proportions of cells expressing KIRs, which constitute a highly polymorphic family of receptors for Human Leukocyte Antigen (HLA) class I. These KIRs delineate a dichotomy in the phenotype and function of γδ T cells. KIR+γδ T cells exhibit characteristics akin to memory like T cells, displaying heightened effector potential, whereas KIR-γδ T cells resemble naïve T cells with comparatively weaker immune responses. Leveraging the assay for transposase-accessible chromatin with sequencing (ATACseq) technology, I show that KIR+γδ T cells have more open loci associated with effector functions than KIR-γδ T cells, which is consistent with the above results. These findings underscore a critical role for KIRs in γδ T cell immunity and hold implications for both our understanding of immune responses influenced by CMV and the potential of γδ T cells in cellular immunotherapy.","abstract_has_math":false,"creators":["Razmi, Mahya"],"institution":"Université d'Ottawa | University of Ottawa","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Djaoud, Zakia"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-07-09T18:04:35Z","date_published":"2024-07-09T18:04:35Z","updated_at":"2026-07-24T03:39:25Z","subjects":["γδ T cell","Killer-cell Immunoglobulin-like Receptors","KIRs","Cytomegalovirus"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://doi.org/10.20381/ruor-30434"],"render_values":[{"text":"https://doi.org/10.20381/ruor-30434","href":"https://doi.org/10.20381/ruor-30434","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10393/46392","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Djaoud, Zakia"]},{"key":"dc:creator","label":"Author","values":["Razmi, Mahya"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2024-07-09T18:04:35Z","2024-07-09"]},{"key":"dc:publisher","label":"Institution","values":["Université d'Ottawa | University of Ottawa"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["γδ T cell","Killer-cell Immunoglobulin-like Receptors","KIRs","Cytomegalovirus"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10393/46392","https://doi.org/10.20381/ruor-30434"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Human circulating γδ T cells are broadly classified into three subsets: Vδ1, Vδ2, and Vδ1/2neg populations, with the Vδ2 subset being the most abundant. In this research, I focus on the contribution and interplay of Natural Killer (NK) receptors to the γδ T cell phenotype and function, with emphasis on Killer-cell Immunoglobulin-like Receptors (KIRs), which are poorly studied in the field of γδ T cell biology. Because cytomegalovirus (CMV) is known to shape the αβ T cell and NK cell repertoires, I studied peripheral blood (PB) γδ T cells from both CMV-seronegative (CMV-) and CMV-seropositive (CMV+) healthy adult humans, using spectral flow cytometry. I found that CMV leaves a stable imprint in the γδ T cell repertoire and phenotype. CMV+ individuals have increased proportions of Vδ1 T cells within the total γδ T cell population. Moreover, γδ T cells from these individuals display increased proportions of cells expressing KIRs, which constitute a highly polymorphic family of receptors for Human Leukocyte Antigen (HLA) class I. These KIRs delineate a dichotomy in the phenotype and function of γδ T cells. KIR+γδ T cells exhibit characteristics akin to memory like T cells, displaying heightened effector potential, whereas KIR-γδ T cells resemble naïve T cells with comparatively weaker immune responses. Leveraging the assay for transposase-accessible chromatin with sequencing (ATACseq) technology, I show that KIR+γδ T cells have more open loci associated with effector functions than KIR-γδ T cells, which is consistent with the above results. These findings underscore a critical role for KIRs in γδ T cell immunity and hold implications for both our understanding of immune responses influenced by CMV and the potential of γδ T cells in cellular immunotherapy."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Killer Cell Immunoglobulin-Like Receptors Delineate Distinct Phenotypes and Functions in Human γδ T Cells"]}]}],"canonical_facts":{"dc:contributor":["Djaoud, Zakia"],"dc:creator":["Razmi, Mahya"],"dc:date":["2024-07-09T18:04:35Z","2024-07-09"],"dc:description":["Human circulating γδ T cells are broadly classified into three subsets: Vδ1, Vδ2, and Vδ1/2neg populations, with the Vδ2 subset being the most abundant. In this research, I focus on the contribution and interplay of Natural Killer (NK) receptors to the γδ T cell phenotype and function, with emphasis on Killer-cell Immunoglobulin-like Receptors (KIRs), which are poorly studied in the field of γδ T cell biology. Because cytomegalovirus (CMV) is known to shape the αβ T cell and NK cell repertoires, I studied peripheral blood (PB) γδ T cells from both CMV-seronegative (CMV-) and CMV-seropositive (CMV+) healthy adult humans, using spectral flow cytometry. I found that CMV leaves a stable imprint in the γδ T cell repertoire and phenotype. CMV+ individuals have increased proportions of Vδ1 T cells within the total γδ T cell population. Moreover, γδ T cells from these individuals display increased proportions of cells expressing KIRs, which constitute a highly polymorphic family of receptors for Human Leukocyte Antigen (HLA) class I. These KIRs delineate a dichotomy in the phenotype and function of γδ T cells. KIR+γδ T cells exhibit characteristics akin to memory like T cells, displaying heightened effector potential, whereas KIR-γδ T cells resemble naïve T cells with comparatively weaker immune responses. Leveraging the assay for transposase-accessible chromatin with sequencing (ATACseq) technology, I show that KIR+γδ T cells have more open loci associated with effector functions than KIR-γδ T cells, which is consistent with the above results. These findings underscore a critical role for KIRs in γδ T cell immunity and hold implications for both our understanding of immune responses influenced by CMV and the potential of γδ T cells in cellular immunotherapy."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10393/46392","https://doi.org/10.20381/ruor-30434"],"dc:language":["en"],"dc:publisher":["Université d'Ottawa | University of Ottawa"],"dc:subject":["γδ T cell","Killer-cell Immunoglobulin-like Receptors","KIRs","Cytomegalovirus"],"dc:title":["Killer Cell Immunoglobulin-Like Receptors Delineate Distinct Phenotypes and Functions in Human γδ T Cells"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T03:39:25Z"}