{"id":{"repo_id":"ottawa-retro","oai_identifier":"oai:ruor.uottawa.ca:10393/27915"},"canonical_url":"https://search.dev.ndltd.org/etd/ottawa-retro/oai:ruor.uottawa.ca:10393/27915","repository":{"repo_id":"ottawa-retro","name":"University of Ottawa","base_url":"https://ruor.uottawa.ca/server/oai/request"},"display":{"title":"Influence of the potency of antigen presentation on CD8+ T cell differentiation and memory","abstract":"CD8+ T cells curtail the proliferation of intracellular pathogens. However, the influence of the potency of stimulation on the development of CD8+ T cell memory is not clear. Using recombinant Ovalbumin (OVA) expressing intracellular bacteria, Mycobacterium bovis (BCG) which induces muted T cell activation, and Listeria monocytogenes (LM) which induces massive T cell activation, the influence of T cell stimulation on memory development was discerned. It was hypothesized that muted activation of CD8+ T cells by BCG might result in impaired development of memory. It was found that in contrast to LM naive as well as memory CD8+ T cells undergo delayed and muted expansion and contraction during BCG infection. Memory CD8+ T cells remained functional as they expressed cytokines, killed targets and proliferated in response to antigen. Furthermore, inflammatory cytokines and T cells curtail antigen-presentation during BCG infection which curbs T cell stimulation and favors the development of functional memory.","abstract_html":"CD8+ T cells curtail the proliferation of intracellular pathogens. However, the influence of the potency of stimulation on the development of CD8+ T cell memory is not clear. Using recombinant Ovalbumin (OVA) expressing intracellular bacteria, Mycobacterium bovis (BCG) which induces muted T cell activation, and Listeria monocytogenes (LM) which induces massive T cell activation, the influence of T cell stimulation on memory development was discerned. It was hypothesized that muted activation of CD8+ T cells by BCG might result in impaired development of memory. It was found that in contrast to LM naive as well as memory CD8+ T cells undergo delayed and muted expansion and contraction during BCG infection. Memory CD8+ T cells remained functional as they expressed cytokines, killed targets and proliferated in response to antigen. Furthermore, inflammatory cytokines and T cells curtail antigen-presentation during BCG infection which curbs T cell stimulation and favors the development of functional memory.","abstract_has_math":false,"creators":["Saldanha, Marsha"],"institution":"University of Ottawa (Canada)","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-11-07T19:02:56Z","date_published":"2013-11-07T19:02:56Z","updated_at":"2026-07-24T03:39:36Z","subjects":["Biology, Microbiology.","Health Sciences, Immunology."],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["Source: Masters Abstracts International, Volume: 47-06, page: 3415.","http://dx.doi.org/10.20381/ruor-18979"],"render_values":[{"text":"Source: Masters Abstracts International, Volume: 47-06, page: 3415.","href":null,"code":true},{"text":"http://dx.doi.org/10.20381/ruor-18979","href":"http://dx.doi.org/10.20381/ruor-18979","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10393/27915","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Saldanha, Marsha"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2013-11-07T19:02:56Z","2007"]},{"key":"dc:publisher","label":"Institution","values":["University of Ottawa (Canada)"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Microbiology.","Health Sciences, Immunology."]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["Source: Masters Abstracts International, Volume: 47-06, page: 3415.","http://hdl.handle.net/10393/27915","http://dx.doi.org/10.20381/ruor-18979"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["CD8+ T cells curtail the proliferation of intracellular pathogens. However, the influence of the potency of stimulation on the development of CD8+ T cell memory is not clear. Using recombinant Ovalbumin (OVA) expressing intracellular bacteria, Mycobacterium bovis (BCG) which induces muted T cell activation, and Listeria monocytogenes (LM) which induces massive T cell activation, the influence of T cell stimulation on memory development was discerned. It was hypothesized that muted activation of CD8+ T cells by BCG might result in impaired development of memory. It was found that in contrast to LM naive as well as memory CD8+ T cells undergo delayed and muted expansion and contraction during BCG infection. Memory CD8+ T cells remained functional as they expressed cytokines, killed targets and proliferated in response to antigen. Furthermore, inflammatory cytokines and T cells curtail antigen-presentation during BCG infection which curbs T cell stimulation and favors the development of functional memory."]},{"key":"dc:format","label":"Dc Format","values":["148 p.","application/pdf"]},{"key":"dc:title","label":"Title","values":["Influence of the potency of antigen presentation on CD8+ T cell differentiation and memory"]}]}],"canonical_facts":{"dc:creator":["Saldanha, Marsha"],"dc:date":["2013-11-07T19:02:56Z","2007"],"dc:description":["CD8+ T cells curtail the proliferation of intracellular pathogens. However, the influence of the potency of stimulation on the development of CD8+ T cell memory is not clear. Using recombinant Ovalbumin (OVA) expressing intracellular bacteria, Mycobacterium bovis (BCG) which induces muted T cell activation, and Listeria monocytogenes (LM) which induces massive T cell activation, the influence of T cell stimulation on memory development was discerned. It was hypothesized that muted activation of CD8+ T cells by BCG might result in impaired development of memory. It was found that in contrast to LM naive as well as memory CD8+ T cells undergo delayed and muted expansion and contraction during BCG infection. Memory CD8+ T cells remained functional as they expressed cytokines, killed targets and proliferated in response to antigen. Furthermore, inflammatory cytokines and T cells curtail antigen-presentation during BCG infection which curbs T cell stimulation and favors the development of functional memory."],"dc:format":["148 p.","application/pdf"],"dc:identifier":["Source: Masters Abstracts International, Volume: 47-06, page: 3415.","http://hdl.handle.net/10393/27915","http://dx.doi.org/10.20381/ruor-18979"],"dc:language":["en"],"dc:publisher":["University of Ottawa (Canada)"],"dc:subject":["Biology, Microbiology.","Health Sciences, Immunology."],"dc:title":["Influence of the potency of antigen presentation on CD8+ T cell differentiation and memory"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T03:39:36Z"}