{"id":{"repo_id":"ottawa-retro","oai_identifier":"oai:ruor.uottawa.ca:10393/27477"},"canonical_url":"https://search.dev.ndltd.org/etd/ottawa-retro/oai:ruor.uottawa.ca:10393/27477","repository":{"repo_id":"ottawa-retro","name":"University of Ottawa","base_url":"https://ruor.uottawa.ca/server/oai/request"},"display":{"title":"Hypoxia-inducible factor-1 alpha (HIF-1alpha) and its role in the hypoxic preconditioning and gene expression patterns in zebrafish ( Danio rerio) embryos","abstract":"Preconditioning to lowered oxygen levels (hypoxia) may occur when an animal is subjected to non-lethal levels of hypoxia and then returned to normoxic conditions. During a subsequent exposure to hypoxia, the pre-exposed animals may exhibit increased tolerance compared to naive animals. I have demonstrated, by analysis of critical PO2's (Pcrit), that zebrafish embryos can be preconditioned to hypoxia. Preconditioned embryos display a lower Pcrit than controls, indicating a heightened ability to endure hypoxic conditions. The role of the hypoxia inducible factor-1 (HIF-1) in promoting hypoxic preconditioning was examined. To determine the role of HIF-1 in preconditioning, embryos deficient in HIF-1alpha (using antisense oligonucleotide morpholinos) were assessed under hypoxic conditions. No significant difference between preconditioning capacities of control- and HIF-1alpha deficient embryos was observed. In addition to assessing Pcrit, a suite of hypoxia responsive genes were analysed by real time PCR. IGFBP-2 showed a significant decrease in expression in the HIF-1alpha deficient embryos, and EPO showed a significant increase in HIF-1alpha deficient embryos. All other genes examined showed no significant change between treated and control embryos.","abstract_html":"Preconditioning to lowered oxygen levels (hypoxia) may occur when an animal is subjected to non-lethal levels of hypoxia and then returned to normoxic conditions. During a subsequent exposure to hypoxia, the pre-exposed animals may exhibit increased tolerance compared to naive animals. I have demonstrated, by analysis of critical PO2's (Pcrit), that zebrafish embryos can be preconditioned to hypoxia. Preconditioned embryos display a lower Pcrit than controls, indicating a heightened ability to endure hypoxic conditions. The role of the hypoxia inducible factor-1 (HIF-1) in promoting hypoxic preconditioning was examined. To determine the role of HIF-1 in preconditioning, embryos deficient in HIF-1alpha (using antisense oligonucleotide morpholinos) were assessed under hypoxic conditions. No significant difference between preconditioning capacities of control- and HIF-1alpha deficient embryos was observed. In addition to assessing Pcrit, a suite of hypoxia responsive genes were analysed by real time PCR. IGFBP-2 showed a significant decrease in expression in the HIF-1alpha deficient embryos, and EPO showed a significant increase in HIF-1alpha deficient embryos. All other genes examined showed no significant change between treated and control embryos.","abstract_has_math":false,"creators":["Mohamed, Amira F"],"institution":"University of Ottawa (Canada)","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-11-07T18:14:19Z","date_published":"2013-11-07T18:14:19Z","updated_at":"2026-07-24T03:39:36Z","subjects":["Biology, General."],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["Source: Masters Abstracts International, Volume: 46-03, page: 1367.","http://dx.doi.org/10.20381/ruor-18728"],"render_values":[{"text":"Source: Masters Abstracts International, Volume: 46-03, page: 1367.","href":null,"code":true},{"text":"http://dx.doi.org/10.20381/ruor-18728","href":"http://dx.doi.org/10.20381/ruor-18728","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10393/27477","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Mohamed, Amira F"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2013-11-07T18:14:19Z","2007"]},{"key":"dc:publisher","label":"Institution","values":["University of Ottawa (Canada)"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, General."]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["Source: Masters Abstracts International, Volume: 46-03, page: 1367.","http://hdl.handle.net/10393/27477","http://dx.doi.org/10.20381/ruor-18728"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Preconditioning to lowered oxygen levels (hypoxia) may occur when an animal is subjected to non-lethal levels of hypoxia and then returned to normoxic conditions. During a subsequent exposure to hypoxia, the pre-exposed animals may exhibit increased tolerance compared to naive animals. I have demonstrated, by analysis of critical PO2's (Pcrit), that zebrafish embryos can be preconditioned to hypoxia. Preconditioned embryos display a lower Pcrit than controls, indicating a heightened ability to endure hypoxic conditions. The role of the hypoxia inducible factor-1 (HIF-1) in promoting hypoxic preconditioning was examined. To determine the role of HIF-1 in preconditioning, embryos deficient in HIF-1alpha (using antisense oligonucleotide morpholinos) were assessed under hypoxic conditions. No significant difference between preconditioning capacities of control- and HIF-1alpha deficient embryos was observed. In addition to assessing Pcrit, a suite of hypoxia responsive genes were analysed by real time PCR. IGFBP-2 showed a significant decrease in expression in the HIF-1alpha deficient embryos, and EPO showed a significant increase in HIF-1alpha deficient embryos. All other genes examined showed no significant change between treated and control embryos."]},{"key":"dc:format","label":"Dc Format","values":["90 p.","application/pdf"]},{"key":"dc:title","label":"Title","values":["Hypoxia-inducible factor-1 alpha (HIF-1alpha) and its role in the hypoxic preconditioning and gene expression patterns in zebrafish ( Danio rerio) embryos"]}]}],"canonical_facts":{"dc:creator":["Mohamed, Amira F"],"dc:date":["2013-11-07T18:14:19Z","2007"],"dc:description":["Preconditioning to lowered oxygen levels (hypoxia) may occur when an animal is subjected to non-lethal levels of hypoxia and then returned to normoxic conditions. During a subsequent exposure to hypoxia, the pre-exposed animals may exhibit increased tolerance compared to naive animals. I have demonstrated, by analysis of critical PO2's (Pcrit), that zebrafish embryos can be preconditioned to hypoxia. Preconditioned embryos display a lower Pcrit than controls, indicating a heightened ability to endure hypoxic conditions. The role of the hypoxia inducible factor-1 (HIF-1) in promoting hypoxic preconditioning was examined. To determine the role of HIF-1 in preconditioning, embryos deficient in HIF-1alpha (using antisense oligonucleotide morpholinos) were assessed under hypoxic conditions. No significant difference between preconditioning capacities of control- and HIF-1alpha deficient embryos was observed. In addition to assessing Pcrit, a suite of hypoxia responsive genes were analysed by real time PCR. IGFBP-2 showed a significant decrease in expression in the HIF-1alpha deficient embryos, and EPO showed a significant increase in HIF-1alpha deficient embryos. All other genes examined showed no significant change between treated and control embryos."],"dc:format":["90 p.","application/pdf"],"dc:identifier":["Source: Masters Abstracts International, Volume: 46-03, page: 1367.","http://hdl.handle.net/10393/27477","http://dx.doi.org/10.20381/ruor-18728"],"dc:language":["en"],"dc:publisher":["University of Ottawa (Canada)"],"dc:subject":["Biology, General."],"dc:title":["Hypoxia-inducible factor-1 alpha (HIF-1alpha) and its role in the hypoxic preconditioning and gene expression patterns in zebrafish ( Danio rerio) embryos"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T03:39:36Z"}