{"id":{"repo_id":"ottawa-retro","oai_identifier":"oai:ruor.uottawa.ca:10393/26744"},"canonical_url":"https://search.dev.ndltd.org/etd/ottawa-retro/oai:ruor.uottawa.ca:10393/26744","repository":{"repo_id":"ottawa-retro","name":"University of Ottawa","base_url":"https://ruor.uottawa.ca/server/oai/request"},"display":{"title":"Isoflavonoid induction and nitrogen assimilation in AM colonized red clover","abstract":"This study postulates that the symbiosis between red clover, Trifolium pratense L., and the arbuscular mycorrhizal (AM) fungus, Glomus intraradices Schenck & Smith, enhances secondary phenolic metabolism and nitrogen assimilation. To test this hypothesis, the four objectives set forth were to determine the impact of AM colonization and/or rhizobial inoculation in red clover on the: (1) growth parameters; (2) nitrogen assimilation, via the activity of glutamine synthetase (GS, E.C. 6.3.1.2), total N content and soluble protein concentration; (3) contents of isoflavonoids (e.g. genistein, daidzein, biochanin A, and formononetin); and (4) contents of these four isoflavonoids over time and with a hormonal factor, jasmonic acid (JA). To accomplish these objectives, four greenhouse experiments were performed. The overall results indicate that the colonized red clover plants, with 22% to 37% of AM root colonization, had enhanced shoot height as well as increased shoot and root dry masses. Co-inoculation with Rhizobium enhanced the growth parameters more than inoculation with either microsymbiont alone. The concentrations of micronutrients significantly increased in red clover with AM colonization and/or rhizobial inoculation, while the macronutrients did not vary to any great degree. (Abstract shortened by UMI.)","abstract_html":"This study postulates that the symbiosis between red clover, Trifolium pratense L., and the arbuscular mycorrhizal (AM) fungus, Glomus intraradices Schenck & Smith, enhances secondary phenolic metabolism and nitrogen assimilation. To test this hypothesis, the four objectives set forth were to determine the impact of AM colonization and/or rhizobial inoculation in red clover on the: (1) growth parameters; (2) nitrogen assimilation, via the activity of glutamine synthetase (GS, E.C. 6.3.1.2), total N content and soluble protein concentration; (3) contents of isoflavonoids (e.g. genistein, daidzein, biochanin A, and formononetin); and (4) contents of these four isoflavonoids over time and with a hormonal factor, jasmonic acid (JA). To accomplish these objectives, four greenhouse experiments were performed. The overall results indicate that the colonized red clover plants, with 22% to 37% of AM root colonization, had enhanced shoot height as well as increased shoot and root dry masses. Co-inoculation with Rhizobium enhanced the growth parameters more than inoculation with either microsymbiont alone. The concentrations of micronutrients significantly increased in red clover with AM colonization and/or rhizobial inoculation, while the macronutrients did not vary to any great degree. (Abstract shortened by UMI.)","abstract_has_math":false,"creators":["Phelps, Debbie"],"institution":"University of Ottawa (Canada)","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-11-07T17:25:51Z","date_published":"2013-11-07T17:25:51Z","updated_at":"2026-07-24T03:39:16Z","subjects":["Biology, Plant Physiology."],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["Source: Masters Abstracts International, Volume: 43-06, page: 2118.","http://dx.doi.org/10.20381/ruor-9771"],"render_values":[{"text":"Source: Masters Abstracts International, Volume: 43-06, page: 2118.","href":null,"code":true},{"text":"http://dx.doi.org/10.20381/ruor-9771","href":"http://dx.doi.org/10.20381/ruor-9771","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10393/26744","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Phelps, Debbie"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2013-11-07T17:25:51Z","2004"]},{"key":"dc:publisher","label":"Institution","values":["University of Ottawa (Canada)"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Plant Physiology."]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["Source: Masters Abstracts International, Volume: 43-06, page: 2118.","http://hdl.handle.net/10393/26744","http://dx.doi.org/10.20381/ruor-9771"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["This study postulates that the symbiosis between red clover, Trifolium pratense L., and the arbuscular mycorrhizal (AM) fungus, Glomus intraradices Schenck & Smith, enhances secondary phenolic metabolism and nitrogen assimilation. To test this hypothesis, the four objectives set forth were to determine the impact of AM colonization and/or rhizobial inoculation in red clover on the: (1) growth parameters; (2) nitrogen assimilation, via the activity of glutamine synthetase (GS, E.C. 6.3.1.2), total N content and soluble protein concentration; (3) contents of isoflavonoids (e.g. genistein, daidzein, biochanin A, and formononetin); and (4) contents of these four isoflavonoids over time and with a hormonal factor, jasmonic acid (JA). To accomplish these objectives, four greenhouse experiments were performed. The overall results indicate that the colonized red clover plants, with 22% to 37% of AM root colonization, had enhanced shoot height as well as increased shoot and root dry masses. Co-inoculation with Rhizobium enhanced the growth parameters more than inoculation with either microsymbiont alone. The concentrations of micronutrients significantly increased in red clover with AM colonization and/or rhizobial inoculation, while the macronutrients did not vary to any great degree. (Abstract shortened by UMI.)"]},{"key":"dc:format","label":"Dc Format","values":["141 p.","application/pdf"]},{"key":"dc:title","label":"Title","values":["Isoflavonoid induction and nitrogen assimilation in AM colonized red clover"]}]}],"canonical_facts":{"dc:creator":["Phelps, Debbie"],"dc:date":["2013-11-07T17:25:51Z","2004"],"dc:description":["This study postulates that the symbiosis between red clover, Trifolium pratense L., and the arbuscular mycorrhizal (AM) fungus, Glomus intraradices Schenck & Smith, enhances secondary phenolic metabolism and nitrogen assimilation. To test this hypothesis, the four objectives set forth were to determine the impact of AM colonization and/or rhizobial inoculation in red clover on the: (1) growth parameters; (2) nitrogen assimilation, via the activity of glutamine synthetase (GS, E.C. 6.3.1.2), total N content and soluble protein concentration; (3) contents of isoflavonoids (e.g. genistein, daidzein, biochanin A, and formononetin); and (4) contents of these four isoflavonoids over time and with a hormonal factor, jasmonic acid (JA). To accomplish these objectives, four greenhouse experiments were performed. The overall results indicate that the colonized red clover plants, with 22% to 37% of AM root colonization, had enhanced shoot height as well as increased shoot and root dry masses. Co-inoculation with Rhizobium enhanced the growth parameters more than inoculation with either microsymbiont alone. The concentrations of micronutrients significantly increased in red clover with AM colonization and/or rhizobial inoculation, while the macronutrients did not vary to any great degree. (Abstract shortened by UMI.)"],"dc:format":["141 p.","application/pdf"],"dc:identifier":["Source: Masters Abstracts International, Volume: 43-06, page: 2118.","http://hdl.handle.net/10393/26744","http://dx.doi.org/10.20381/ruor-9771"],"dc:language":["en"],"dc:publisher":["University of Ottawa (Canada)"],"dc:subject":["Biology, Plant Physiology."],"dc:title":["Isoflavonoid induction and nitrogen assimilation in AM colonized red clover"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T03:39:16Z"}