Electrochemical and spectroscopic analysis of collagen films and lipid bilayers on biomaterial surfaces

One crucial step towards biointegration of an implant material into the human body is the adsorption of proteins. This thesis aims at the development of a stable and relatively simple system to model the native protein film on a biomaterial surface under the influence of electric fields. Collagen was chosen as the adsorbing protein and gold as the substrate material. Capacitance measurements, polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS), surface plasmon resonance spectroscopy (SPR) and ellipsometry are employed to analyze the stability and structure of adsorbed collagen films. The adsorbed collagen films are stable in contact with air and in contact with an electrolyte solution. The thermal stability of the collagen molecules is strongly enhanced when adsorbed on a gold surface. A potential-dependent incorporation of the electrolyte solution into the adsorbed collagen film is observed. As enhancements of the model system, other substrate materials like Ti or TiOxCy are introduced and compared to the gold substrates. The transfer of a DMPC lipid bilayer onto the collagen covered gold surface to simulate the adhesion of a cell membrane is another tested extension of the model system.

Level thesis:degree_level

thesis.doctoral

Grantor dc:publisher

Universität Oldenburg

Year

2013

Author dc:creator

• Ahlers, Michael

Contributors dc:contributor

• Wittstock, Gunther
• Klüner, Thorsten
• Brand, Izabella

• Kollagen, Biomaterial, Infrarot-Reflexions-Absorptions-Spektroskopie, Elektrochemie

Repository record source_url

http://oops.uni-oldenburg.de/1459

OAI identifier oai:identifier

oai:oops.uni-oldenburg.de:1459