{"id":{"repo_id":"ohiolink","oai_identifier":"oai:etd.ohiolink.edu:wright1364484571"},"canonical_url":"https://search.dev.ndltd.org/etd/ohiolink/oai:etd.ohiolink.edu:wright1364484571","repository":{"repo_id":"ohiolink","name":"OhioLINK","base_url":"https://etd.ohiolink.edu/acprod/odb_etd/ws/oai/oai"},"display":{"title":"Effect of Rat Strain Stereotactic Coordinates on Infarct Volume","abstract":"Ischemic stroke makes up 87% of all hospital-admitted stroke cases annually; the primary treatment for these cases is intravenous administration of tPA within a 3.5 hour window from stroke onset. A long-term delayed ischemic stroke treatment proposed by this study was a combination of the pharmaceuticals Fluoxetine (SSRI), Simvastatin (statin), and ascorbic acid (Vitamin C). 51 adult rat subjects (10-12 months of age; 44 Sprague Dawley, 7 Long Evans) were given a combination of the drugs for 31 days. Drugs were given through voluntary oral administration via sugar cookie-dough balls to reduce inhibition of neurogenesis through stress-related glucocorticoid production. Drug combinations were as follows: FSA - 5 mg/kg fluoxetine, 0.5 mg/kg simvastatin and 20 mg/kg ascorbic acid; FS - 5 mg/kg fluoxetine, 0.5 mg/kg simvastatin; and the vehicle control. Endothelin-induced cortical stroke was administered using 2 different set of coordinates relative to bregma: Group 1 - (AP: 0.0 mm, ML: -2.5 mm) and (AP +1.5 mm; ML: -2.5 mm); Group 2 - (AP: 0.0 mm, ML: -2.5mm) and (AP +2.3; ML -2.5 mm). To analyze functional deficit, rats were subject to Montoya Staircase functional test once pre-stroke and twice post-stroke, and the Forelimb Asymmetry functional test once pre-stroke and thrice post-stroke. Results showed that Long Evans rats sustain a significantly larger infarct volume compared to Sprague Dawley rats using Group 2 coordinates; Group 1 cortical injection coordinates produced a larger infarct than Group 2 coordinates in FSA Sprague Dawley rats; drug treatment showed no effect on total infarct volume, however, this may be attributed to use of generic fluoxetine in Group 2 Sprague Dawley rats.","abstract_html":"Ischemic stroke makes up 87% of all hospital-admitted stroke cases annually; the primary treatment for these cases is intravenous administration of tPA within a 3.5 hour window from stroke onset. A long-term delayed ischemic stroke treatment proposed by this study was a combination of the pharmaceuticals Fluoxetine (SSRI), Simvastatin (statin), and ascorbic acid (Vitamin C). 51 adult rat subjects (10-12 months of age; 44 Sprague Dawley, 7 Long Evans) were given a combination of the drugs for 31 days. Drugs were given through voluntary oral administration via sugar cookie-dough balls to reduce inhibition of neurogenesis through stress-related glucocorticoid production. Drug combinations were as follows: FSA - 5 mg/kg fluoxetine, 0.5 mg/kg simvastatin and 20 mg/kg ascorbic acid; FS - 5 mg/kg fluoxetine, 0.5 mg/kg simvastatin; and the vehicle control. Endothelin-induced cortical stroke was administered using 2 different set of coordinates relative to bregma: Group 1 - (AP: 0.0 mm, ML: -2.5 mm) and (AP +1.5 mm; ML: -2.5 mm); Group 2 - (AP: 0.0 mm, ML: -2.5mm) and (AP +2.3; ML -2.5 mm). To analyze functional deficit, rats were subject to Montoya Staircase functional test once pre-stroke and twice post-stroke, and the Forelimb Asymmetry functional test once pre-stroke and thrice post-stroke. Results showed that Long Evans rats sustain a significantly larger infarct volume compared to Sprague Dawley rats using Group 2 coordinates; Group 1 cortical injection coordinates produced a larger infarct than Group 2 coordinates in FSA Sprague Dawley rats; drug treatment showed no effect on total infarct volume, however, this may be attributed to use of generic fluoxetine in Group 2 Sprague Dawley rats.","abstract_has_math":false,"creators":["Sanghvi, Saagar K."],"institution":"Wright State University","degree_name":"Master of Science (MS)","degree_level":"masters","degree_discipline":"Anatomy","degree_department":null,"school":null,"contributors":["Corbett, Adrian"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-05-01","date_published":"2013-05-01","updated_at":"2026-07-24T03:37:16Z","subjects":["Neurosciences","Neurology","Neurobiology","Physiology","Anatomy and Physiology","stroke","infarct volume","rat strain","Montoya Staircase","ischemia","fluoxetine","simvastatin","sub-ventricular zone","stereotaxic coordinates"],"languages":["English"],"rights":["unrestricted","This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://rave.ohiolink.edu/etdc/view?acc_num=wright1364484571","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Corbett, Adrian"]},{"key":"dc:creator","label":"Author","values":["Sanghvi, Saagar K."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2013-05-01"]},{"key":"dc:publisher","label":"Institution","values":["Wright State University / OhioLINK"]},{"key":"dc:type","label":"Dc Type","values":["Electronic Thesis or Dissertation"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Anatomy"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Wright State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Neurosciences","Neurology","Neurobiology","Physiology","Anatomy and Physiology","stroke","infarct volume","rat strain","Montoya Staircase","ischemia","fluoxetine","simvastatin","sub-ventricular zone","stereotaxic coordinates"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:rights","label":"Dc Rights","values":["unrestricted","This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://rave.ohiolink.edu/etdc/view?acc_num=wright1364484571"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Ischemic stroke makes up 87% of all hospital-admitted stroke cases annually; the primary treatment for these cases is intravenous administration of tPA within a 3.5 hour window from stroke onset. A long-term delayed ischemic stroke treatment proposed by this study was a combination of the pharmaceuticals Fluoxetine (SSRI), Simvastatin (statin), and ascorbic acid (Vitamin C). 51 adult rat subjects (10-12 months of age; 44 Sprague Dawley, 7 Long Evans) were given a combination of the drugs for 31 days. Drugs were given through voluntary oral administration via sugar cookie-dough balls to reduce inhibition of neurogenesis through stress-related glucocorticoid production. Drug combinations were as follows: FSA - 5 mg/kg fluoxetine, 0.5 mg/kg simvastatin and 20 mg/kg ascorbic acid; FS - 5 mg/kg fluoxetine, 0.5 mg/kg simvastatin; and the vehicle control. Endothelin-induced cortical stroke was administered using 2 different set of coordinates relative to bregma: Group 1 - (AP: 0.0 mm, ML: -2.5 mm) and (AP +1.5 mm; ML: -2.5 mm); Group 2 - (AP: 0.0 mm, ML: -2.5mm) and (AP +2.3; ML -2.5 mm). To analyze functional deficit, rats were subject to Montoya Staircase functional test once pre-stroke and twice post-stroke, and the Forelimb Asymmetry functional test once pre-stroke and thrice post-stroke. Results showed that Long Evans rats sustain a significantly larger infarct volume compared to Sprague Dawley rats using Group 2 coordinates; Group 1 cortical injection coordinates produced a larger infarct than Group 2 coordinates in FSA Sprague Dawley rats; drug treatment showed no effect on total infarct volume, however, this may be attributed to use of generic fluoxetine in Group 2 Sprague Dawley rats."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf","p.70","13.78 MB"]},{"key":"dc:title","label":"Title","values":["Effect of Rat Strain Stereotactic Coordinates on Infarct Volume"]}]}],"canonical_facts":{"dc:contributor":["Corbett, Adrian"],"dc:creator":["Sanghvi, Saagar K."],"dc:date":["2013-05-01"],"dc:description":["Ischemic stroke makes up 87% of all hospital-admitted stroke cases annually; the primary treatment for these cases is intravenous administration of tPA within a 3.5 hour window from stroke onset. A long-term delayed ischemic stroke treatment proposed by this study was a combination of the pharmaceuticals Fluoxetine (SSRI), Simvastatin (statin), and ascorbic acid (Vitamin C). 51 adult rat subjects (10-12 months of age; 44 Sprague Dawley, 7 Long Evans) were given a combination of the drugs for 31 days. Drugs were given through voluntary oral administration via sugar cookie-dough balls to reduce inhibition of neurogenesis through stress-related glucocorticoid production. Drug combinations were as follows: FSA - 5 mg/kg fluoxetine, 0.5 mg/kg simvastatin and 20 mg/kg ascorbic acid; FS - 5 mg/kg fluoxetine, 0.5 mg/kg simvastatin; and the vehicle control. Endothelin-induced cortical stroke was administered using 2 different set of coordinates relative to bregma: Group 1 - (AP: 0.0 mm, ML: -2.5 mm) and (AP +1.5 mm; ML: -2.5 mm); Group 2 - (AP: 0.0 mm, ML: -2.5mm) and (AP +2.3; ML -2.5 mm). To analyze functional deficit, rats were subject to Montoya Staircase functional test once pre-stroke and twice post-stroke, and the Forelimb Asymmetry functional test once pre-stroke and thrice post-stroke. Results showed that Long Evans rats sustain a significantly larger infarct volume compared to Sprague Dawley rats using Group 2 coordinates; Group 1 cortical injection coordinates produced a larger infarct than Group 2 coordinates in FSA Sprague Dawley rats; drug treatment showed no effect on total infarct volume, however, this may be attributed to use of generic fluoxetine in Group 2 Sprague Dawley rats."],"dc:format":["application/pdf","p.70","13.78 MB"],"dc:identifier":["http://rave.ohiolink.edu/etdc/view?acc_num=wright1364484571"],"dc:language":["English"],"dc:publisher":["Wright State University / OhioLINK"],"dc:rights":["unrestricted","This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws."],"dc:subject":["Neurosciences","Neurology","Neurobiology","Physiology","Anatomy and Physiology","stroke","infarct volume","rat strain","Montoya Staircase","ischemia","fluoxetine","simvastatin","sub-ventricular zone","stereotaxic coordinates"],"dc:title":["Effect of Rat Strain Stereotactic Coordinates on Infarct Volume"],"dc:type":["Electronic Thesis or Dissertation"],"thesis:degree_discipline":["Anatomy"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science (MS)"],"thesis:institution_name":["Wright State University"]},"updated_at":"2026-07-24T03:37:16Z"}