Biofilm Removal with Acoustic Cavitation and Lavage

<p>Bacterial biofilm colonies attached to orthopedic implants, and surrounding tissues resist to antibiotic treatments, prolong hospitalization, increase mortality, and usually require the removal and replacement of the entire joint. Through acoustic cavitation, it could be possible to remove the biofilm colonies from the infected surface, thus reducing healing time and even eliminating the need to remove and replace the implant. </p><p>In this thesis, biofilm formation was first induced in standard 96-well round –bottom microplates. These microplates were then exposed to ultrasonic cavitation and pulsed lavage for the same period, and the cleaning effectiveness of two methods were assessed. The results showed that an ultrasonic transducer can be effective in removing biofilm from the surface.</p><p>Consequently, a new device that uses the combined effects of acoustic cavitation and lavage to disrupt bilfilms was developed. This device consists of a ring-shaped sonicator inserted into the cone of a pulsed lavage apparatus. A function generator connected to an amplifier drives the sonicator.</p><p>Finally, two different methods (pulsed lavage and the combination of sonicator and pulsed lavage) were used to clean the biofilm. The remaining bacterial biofilm was then removed by using a bath sonicator, and streaked onto TSB/agar Petri dishes to assess cleaning effectiveness. The results demonstrated that acoustic cavitation in conjunction with lavage effectively can remove E.coli biofilm adhered on surfaces better than pulsed lavage alone without visibly damaging these surfaces.</p>

Name thesis:degree_name

Master of Science in Engineering

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Mechanical Engineering

Grantor dc:publisher

University of Toledo

Year dc:date

2013

Author dc:creator

• Zhang, Siyuan

Contributors dc:contributor

• Cioc, Sorin

• Acoustics
• Biology
• Engineering
• Health Sciences
• sonication
• pulsed lavage
• biofilm
• cavitation

Statement dc:rights

• unrestricted
• This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.

Language dc:language

English

Repository record dc:identifier

http://rave.ohiolink.edu/etdc/view?acc_num=toledo1356194652

OAI identifier oai:identifier

oai:etd.ohiolink.edu:toledo1356194652