Synthesis of Bisubstrate Analog Inhibitors against Aspartate Semialdehyde Dehydrogenase

Aspartate semialdehyde dehydrogenase (ASADH) is an essential enzyme found in bacteria, fungi and plants. This enzyme is responsible for the production of several essential amino acids, and its absence in humans makes ASADH an attractive target for the development of antibiotics. The goal of the project is to design, synthesize and build species-selectivity into bisubstrate analogue inhibitors against ASADH. Analogues were choosen to synthesize based on the docking model predictions for binding affinities and selectivities against ASADHs that have been purified and characterized from different microbial species. The synthetic approach involves the parallel synthesis of secondary amine derivatives and phenyl alkyl halides. The two synthons are being coupled through nucleophilic substitution followed by hydrolysis to form the desired bisubstrate analogues. Four compounds were identified based on docking score and selectivity analyses. These analogs were synthesized and tested against ASADH. These studies have led to identification of sub-millimolar inhibitors of ASADH.This work is supported by a grant from the NIH (AI077720).

Name thesis:degree_name

Master of Science

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Chemistry

Grantor dc:publisher

University of Toledo

Year dc:date

2012

Author dc:creator

• Muvvala, Harinath G.

Contributors dc:contributor

• Viola, Ronald E.

• Biochemistry

Statement dc:rights

• unrestricted
• This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.

Language dc:language

English

Repository record dc:identifier

http://rave.ohiolink.edu/etdc/view?acc_num=toledo1352438577

OAI identifier oai:identifier

oai:etd.ohiolink.edu:toledo1352438577