Incorporation of selected plant extracts into edible chitosan films and the effect on the antiviral, antibacterial and mechanical properties of the material
type: CreativeWork
description: Edible films and coatings are increasingly being used as carriers of functional additives including antimicrobial agents. Consumer interest in naturally-derived antimicrobials has also increased. Plant extracts such as grape seed and green tea extracts are known to have antiviral as well as antibacterial activities. Even though there are several researches that have investigated the use of edible films and coatings as carriers of antimicrobial agents against foodborne bacterial pathogens, unfortunately data is lacking on the use of these same technologies on foodborne viruses. Therefore, this study seeks to develop edible films and coatings that can control foodborne viruses. The film’s antibacterial and mechanical properties were also tested. Chapters 1 and 2 introduce and review previous researches in edible films and coatings. Topics such as the types of biopolymer, film formation methods and mechanisms, foodborne viruses and bacteria, as well as edible film characterization and properties are discussed.Chapter 3 of this study investigated the virucidal activity of green tea extract (GTE) dissolved in deionized water and also incorporated into chitosan film forming solutions (FFS) and into chitosan films. For comparison, the antibacterial activity of the films was also investigated against Listeria innocua and Escherichia coli K12. The viral infectivity after treatments was measured by plaque assays. The 1, 1.5 and 2.5% aqueous GTE solutions significantly (p<0.05) reduced murine norovirus (MNV-1) plaques by 1.69, 2.47, and 3.26 log after 3 h exposure, respectively. Similarly, the FFS containing 2.5 and 5.0% GTE reduced MNV-1 counts by 2.45 and 3.97 log10 PFU/ml, respectively after 3 h exposure. Additionally, the edible films enriched with the GTE (5, 10 and 15%) were also effective against MNV-1. After a 24 h incubation period, the 5 and 10% GTE films significantly (p<0.05) resulted in MNV-1 titer reductions of 1.60 and 4.50 log10 PFU/ml, respectively. The film c…
abstract: Edible films and coatings are increasingly being used as carriers of functional additives including antimicrobial agents. Consumer interest in naturally-derived antimicrobials has also increased. Plant extracts such as grape seed and green tea extracts are known to have antiviral as well as antibacterial activities. Even though there are several researches that have investigated the use of edible films and coatings as carriers of antimicrobial agents against foodborne bacterial pathogens, unfortunately data is lacking on the use of these same technologies on foodborne viruses. Therefore, this study seeks to develop edible films and coatings that can control foodborne viruses. The film’s antibacterial and mechanical properties were also tested. Chapters 1 and 2 introduce and review previous researches in edible films and coatings. Topics such as the types of biopolymer, film formation methods and mechanisms, foodborne viruses and bacteria, as well as edible film characterization and properties are discussed.Chapter 3 of this study investigated the virucidal activity of green tea extract (GTE) dissolved in deionized water and also incorporated into chitosan film forming solutions (FFS) and into chitosan films. For comparison, the antibacterial activity of the films was also investigated against Listeria innocua and Escherichia coli K12. The viral infectivity after treatments was measured by plaque assays. The 1, 1.5 and 2.5% aqueous GTE solutions significantly (p<0.05) reduced murine norovirus (MNV-1) plaques by 1.69, 2.47, and 3.26 log after 3 h exposure, respectively. Similarly, the FFS containing 2.5 and 5.0% GTE reduced MNV-1 counts by 2.45 and 3.97 log10 PFU/ml, respectively after 3 h exposure. Additionally, the edible films enriched with the GTE (5, 10 and 15%) were also effective against MNV-1. After a 24 h incubation period, the 5 and 10% GTE films significantly (p<0.05) resulted in MNV-1 titer reductions of 1.60 and 4.50 log10 PFU/ml, respectively. The film c…
author: Amankwaah, Collins
language: English
keywords: Food Science, Edible film, Edible coating, antiviral packaging, edible packaging, antimicrobial packaging, foodborne viruses, antiviral edible film, plant extracts, green tea extracts, grape seed extracts, natural antimicrobial agents
date published: 2013-08-08
date modified: 2026-07-24T03:37:46Z
provider: OhioLINK
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: The Ohio State University