{"id":{"repo_id":"ohiolink","oai_identifier":"oai:etd.ohiolink.edu:osu1366204177"},"canonical_url":"https://search.dev.ndltd.org/etd/ohiolink/oai:etd.ohiolink.edu:osu1366204177","repository":{"repo_id":"ohiolink","name":"OhioLINK","base_url":"https://etd.ohiolink.edu/acprod/odb_etd/ws/oai/oai"},"display":{"title":"<i>Maize fine streak virus</i> (MFSV) gene expression and protein interaction","abstract":"<i>Maize fine streak virus</i> (MFSV) belongs to the family Rhabdoviridae, and is transmitted by the leafhopper <i>Graminella nigrifrons</i>. The MFSV genome is a 13,782 nucleotide, non-segmented, negative-sense RNA that encodes five core structural proteins: the nucleocapsid protein (N), the phosphoprotein (P), the polymerase (L), the matrix protein (M) and the glycoprotein (G) and two non-structural proteins, MFSV 3 and 4. The genes follow the order 3'-N-P-3-4-M-G-L-5’. Functions for the MFSV N, P, M, G, and L genes have been assigned based on sequence homologies to other Rhabdoviridae, but functions for the non-structural MFSV 3 and 4 genes remain unknown. To get insights of the relative importance of MFSV 3 and 4 in virus replication and systemic infection, we analyzed the accumulation of the MFSV 3 and 4 transcripts relative to the N transcripts in maize and <i>G. nigrifrons</i> using RT-qPCR assays. MFSV 3 transcripts accumulated at higher level than N transcripts in both plant and insect hosts, whereas MFSV 4 transcripts accumulated at similar levels than N transcripts in maize but at lower levels in <i>G. nigrifrons</i>. Our results indicate that the regulation of MFSV gene transcription is different from that of animal-infecting rhabdoviruses, for which transcript accumulation decreases sequentially from the 3’ to the 5’ end of the genome. To determine whether the MFSV 3 and 4 protein function requires interaction with other MFSV proteins (e.g., for virus movement in plants), a protein-protein interaction map was generated by means of yeast two-hybrid (YTH) and bimolecular fluorescence complementation (BiFC). The MFSV 3 and 4 proteins strongly interacted with each other in both assays, suggesting that this interaction is likely to occur in nature. Based on the location of MFSV 4 in the genome, the size of the encoded protein and higher accumulation of transcripts in the plant compared to the insect host, MFSV 4 may be a movement protein, and its strong interaction with MFSV 3 may be important during infection of the plant host.","abstract_html":"&lt;i&gt;Maize fine streak virus&lt;/i&gt; (MFSV) belongs to the family Rhabdoviridae, and is transmitted by the leafhopper &lt;i&gt;Graminella nigrifrons&lt;/i&gt;. The MFSV genome is a 13,782 nucleotide, non-segmented, negative-sense RNA that encodes five core structural proteins: the nucleocapsid protein (N), the phosphoprotein (P), the polymerase (L), the matrix protein (M) and the glycoprotein (G) and two non-structural proteins, MFSV 3 and 4. The genes follow the order 3&#x27;-N-P-3-4-M-G-L-5’. Functions for the MFSV N, P, M, G, and L genes have been assigned based on sequence homologies to other Rhabdoviridae, but functions for the non-structural MFSV 3 and 4 genes remain unknown. To get insights of the relative importance of MFSV 3 and 4 in virus replication and systemic infection, we analyzed the accumulation of the MFSV 3 and 4 transcripts relative to the N transcripts in maize and &lt;i&gt;G. nigrifrons&lt;/i&gt; using RT-qPCR assays. MFSV 3 transcripts accumulated at higher level than N transcripts in both plant and insect hosts, whereas MFSV 4 transcripts accumulated at similar levels than N transcripts in maize but at lower levels in &lt;i&gt;G. nigrifrons&lt;/i&gt;. Our results indicate that the regulation of MFSV gene transcription is different from that of animal-infecting rhabdoviruses, for which transcript accumulation decreases sequentially from the 3’ to the 5’ end of the genome. To determine whether the MFSV 3 and 4 protein function requires interaction with other MFSV proteins (e.g., for virus movement in plants), a protein-protein interaction map was generated by means of yeast two-hybrid (YTH) and bimolecular fluorescence complementation (BiFC). The MFSV 3 and 4 proteins strongly interacted with each other in both assays, suggesting that this interaction is likely to occur in nature. Based on the location of MFSV 4 in the genome, the size of the encoded protein and higher accumulation of transcripts in the plant compared to the insect host, MFSV 4 may be a movement protein, and its strong interaction with MFSV 3 may be important during infection of the plant host.","abstract_has_math":false,"creators":["Cisneros Delgadillo, Fiorella Melina"],"institution":"The Ohio State University","degree_name":"Doctor of Philosophy","degree_level":"doctoral","degree_discipline":"Plant Pathology","degree_department":null,"school":null,"contributors":["Redinbaugh, Margaret","Qu, Feng"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-08-08","date_published":"2013-08-08","updated_at":"2026-07-24T03:37:46Z","subjects":["Virology","Plant Pathology","Molecular Biology","Rhabdoviruses","Rt-qPCR","protein interaction","viral vectors"],"languages":["English"],"rights":["unrestricted","This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://rave.ohiolink.edu/etdc/view?acc_num=osu1366204177","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Redinbaugh, Margaret","Qu, Feng"]},{"key":"dc:creator","label":"Author","values":["Cisneros Delgadillo, Fiorella Melina"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2013-08-08"]},{"key":"dc:publisher","label":"Institution","values":["The Ohio State University / OhioLINK"]},{"key":"dc:type","label":"Dc Type","values":["Electronic Thesis or Dissertation"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Plant Pathology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["doctoral"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["The Ohio State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Virology","Plant Pathology","Molecular Biology","Rhabdoviruses","Rt-qPCR","protein interaction","viral vectors"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:rights","label":"Dc Rights","values":["unrestricted","This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://rave.ohiolink.edu/etdc/view?acc_num=osu1366204177"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["<i>Maize fine streak virus</i> (MFSV) belongs to the family Rhabdoviridae, and is transmitted by the leafhopper <i>Graminella nigrifrons</i>. The MFSV genome is a 13,782 nucleotide, non-segmented, negative-sense RNA that encodes five core structural proteins: the nucleocapsid protein (N), the phosphoprotein (P), the polymerase (L), the matrix protein (M) and the glycoprotein (G) and two non-structural proteins, MFSV 3 and 4. The genes follow the order 3'-N-P-3-4-M-G-L-5’. Functions for the MFSV N, P, M, G, and L genes have been assigned based on sequence homologies to other Rhabdoviridae, but functions for the non-structural MFSV 3 and 4 genes remain unknown. To get insights of the relative importance of MFSV 3 and 4 in virus replication and systemic infection, we analyzed the accumulation of the MFSV 3 and 4 transcripts relative to the N transcripts in maize and <i>G. nigrifrons</i> using RT-qPCR assays. MFSV 3 transcripts accumulated at higher level than N transcripts in both plant and insect hosts, whereas MFSV 4 transcripts accumulated at similar levels than N transcripts in maize but at lower levels in <i>G. nigrifrons</i>. Our results indicate that the regulation of MFSV gene transcription is different from that of animal-infecting rhabdoviruses, for which transcript accumulation decreases sequentially from the 3’ to the 5’ end of the genome. To determine whether the MFSV 3 and 4 protein function requires interaction with other MFSV proteins (e.g., for virus movement in plants), a protein-protein interaction map was generated by means of yeast two-hybrid (YTH) and bimolecular fluorescence complementation (BiFC). The MFSV 3 and 4 proteins strongly interacted with each other in both assays, suggesting that this interaction is likely to occur in nature. Based on the location of MFSV 4 in the genome, the size of the encoded protein and higher accumulation of transcripts in the plant compared to the insect host, MFSV 4 may be a movement protein, and its strong interaction with MFSV 3 may be important during infection of the plant host."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf","p.150","2.07 MB"]},{"key":"dc:title","label":"Title","values":["<i>Maize fine streak virus</i> (MFSV) gene expression and protein interaction"]}]}],"canonical_facts":{"dc:contributor":["Redinbaugh, Margaret","Qu, Feng"],"dc:creator":["Cisneros Delgadillo, Fiorella Melina"],"dc:date":["2013-08-08"],"dc:description":["<i>Maize fine streak virus</i> (MFSV) belongs to the family Rhabdoviridae, and is transmitted by the leafhopper <i>Graminella nigrifrons</i>. The MFSV genome is a 13,782 nucleotide, non-segmented, negative-sense RNA that encodes five core structural proteins: the nucleocapsid protein (N), the phosphoprotein (P), the polymerase (L), the matrix protein (M) and the glycoprotein (G) and two non-structural proteins, MFSV 3 and 4. The genes follow the order 3'-N-P-3-4-M-G-L-5’. Functions for the MFSV N, P, M, G, and L genes have been assigned based on sequence homologies to other Rhabdoviridae, but functions for the non-structural MFSV 3 and 4 genes remain unknown. To get insights of the relative importance of MFSV 3 and 4 in virus replication and systemic infection, we analyzed the accumulation of the MFSV 3 and 4 transcripts relative to the N transcripts in maize and <i>G. nigrifrons</i> using RT-qPCR assays. MFSV 3 transcripts accumulated at higher level than N transcripts in both plant and insect hosts, whereas MFSV 4 transcripts accumulated at similar levels than N transcripts in maize but at lower levels in <i>G. nigrifrons</i>. Our results indicate that the regulation of MFSV gene transcription is different from that of animal-infecting rhabdoviruses, for which transcript accumulation decreases sequentially from the 3’ to the 5’ end of the genome. To determine whether the MFSV 3 and 4 protein function requires interaction with other MFSV proteins (e.g., for virus movement in plants), a protein-protein interaction map was generated by means of yeast two-hybrid (YTH) and bimolecular fluorescence complementation (BiFC). The MFSV 3 and 4 proteins strongly interacted with each other in both assays, suggesting that this interaction is likely to occur in nature. Based on the location of MFSV 4 in the genome, the size of the encoded protein and higher accumulation of transcripts in the plant compared to the insect host, MFSV 4 may be a movement protein, and its strong interaction with MFSV 3 may be important during infection of the plant host."],"dc:format":["application/pdf","p.150","2.07 MB"],"dc:identifier":["http://rave.ohiolink.edu/etdc/view?acc_num=osu1366204177"],"dc:language":["English"],"dc:publisher":["The Ohio State University / OhioLINK"],"dc:rights":["unrestricted","This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center."],"dc:subject":["Virology","Plant Pathology","Molecular Biology","Rhabdoviruses","Rt-qPCR","protein interaction","viral vectors"],"dc:title":["<i>Maize fine streak virus</i> (MFSV) gene expression and protein interaction"],"dc:type":["Electronic Thesis or Dissertation"],"thesis:degree_discipline":["Plant Pathology"],"thesis:degree_level":["doctoral"],"thesis:degree_name":["Doctor of Philosophy"],"thesis:institution_name":["The Ohio State University"]},"updated_at":"2026-07-24T03:37:46Z"}