{"id":{"repo_id":"ohiolink","oai_identifier":"oai:etd.ohiolink.edu:osu1366099986"},"canonical_url":"https://search.dev.ndltd.org/etd/ohiolink/oai:etd.ohiolink.edu:osu1366099986","repository":{"repo_id":"ohiolink","name":"OhioLINK","base_url":"https://etd.ohiolink.edu/acprod/odb_etd/ws/oai/oai"},"display":{"title":"Identification of Anti-inflammatory and Antioxidant Properties of MangostinXanthones in Adipocyte Reporter Assays","abstract":"We developed three fluorescence biosensors based on a 3T3-L1 cell line that stably expressed nuclear factor κB response element (<i>Nfkb</i>-RE)/GFP, fatty acid binding protein 4 promoter (<i>Fabp4</i>-P)/CFP, and nuclear factor erythroid 2-related factor2 promoter (<i>Nrf2</i>-P)/YFP fluorescent reporters. We hypothesized that fluorescent signals directly correlated to inflammatory, adipogenic, and antioxidant status in adipocytes, respectively. Two polyphenolic xanthones, α-mangostin (α-MG) and γ-mangostin (γ-MG) from mangosteen fruit, have been reported to have anti-adipogenic and anti-inflammatory activities. We validated inflammatory and adipogenic properties of the major mangosteen xanthone α-MG using a <i>Nfkb</i>-RE/GFP biosensor assay, traditional ELISA, and semi-quantitative PCR approach. We also identified unique properties of γ-MG, a minor xanthone in mangosteen fruit. γ-MG suppressed adipogenesis and the anti-inflammatory cytokine adiponectin, but retained its ability to suppress the <i>Nfkb</i>-RE/GFP reporter and reduced the secretion of MCP-1 as compared to control adipocytes. To gain insight into mechanisms involved in the inhibition of adipogenesis, we compared the effects of α-MG on the activation of PPARγ and its target gene <i>Fabp4</i>, and Nrf2, an inducer of <i>Pparg</i> expression. The screening in <i>Nrf2</i>-P/YFP adipocytes suggested that α-MG effectively and dose-dependently reduced <i>Nrf2</i> promoter activation, whereas <i>Fabp4</i> promoter activation was not influenced. Thus, the antioxidant response of α-MG appears to be an early mechanism for the suppression of adipogenic and inflammatory effects in adipocytes. The combined application of high-throughput fluorescence biosensors can provide an effective platform for the identification of nutraceuticals and the mechanism of their actions in adipocytes.","abstract_html":"We developed three fluorescence biosensors based on a 3T3-L1 cell line that stably expressed nuclear factor κB response element (&lt;i&gt;Nfkb&lt;/i&gt;-RE)/GFP, fatty acid binding protein 4 promoter (&lt;i&gt;Fabp4&lt;/i&gt;-P)/CFP, and nuclear factor erythroid 2-related factor2 promoter (&lt;i&gt;Nrf2&lt;/i&gt;-P)/YFP fluorescent reporters. We hypothesized that fluorescent signals directly correlated to inflammatory, adipogenic, and antioxidant status in adipocytes, respectively. Two polyphenolic xanthones, α-mangostin (α-MG) and γ-mangostin (γ-MG) from mangosteen fruit, have been reported to have anti-adipogenic and anti-inflammatory activities. We validated inflammatory and adipogenic properties of the major mangosteen xanthone α-MG using a &lt;i&gt;Nfkb&lt;/i&gt;-RE/GFP biosensor assay, traditional ELISA, and semi-quantitative PCR approach. We also identified unique properties of γ-MG, a minor xanthone in mangosteen fruit. γ-MG suppressed adipogenesis and the anti-inflammatory cytokine adiponectin, but retained its ability to suppress the &lt;i&gt;Nfkb&lt;/i&gt;-RE/GFP reporter and reduced the secretion of MCP-1 as compared to control adipocytes. To gain insight into mechanisms involved in the inhibition of adipogenesis, we compared the effects of α-MG on the activation of PPARγ and its target gene &lt;i&gt;Fabp4&lt;/i&gt;, and Nrf2, an inducer of &lt;i&gt;Pparg&lt;/i&gt; expression. The screening in &lt;i&gt;Nrf2&lt;/i&gt;-P/YFP adipocytes suggested that α-MG effectively and dose-dependently reduced &lt;i&gt;Nrf2&lt;/i&gt; promoter activation, whereas &lt;i&gt;Fabp4&lt;/i&gt; promoter activation was not influenced. Thus, the antioxidant response of α-MG appears to be an early mechanism for the suppression of adipogenic and inflammatory effects in adipocytes. The combined application of high-throughput fluorescence biosensors can provide an effective platform for the identification of nutraceuticals and the mechanism of their actions in adipocytes.","abstract_has_math":false,"creators":["Shen, Qiwen"],"institution":"The Ohio State University","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Human Ecology: Human Nutrition","degree_department":null,"school":null,"contributors":["Ziouzenkova, Ouliana"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-07-24","date_published":"2013-07-24","updated_at":"2026-07-24T03:37:46Z","subjects":["Nutrition","adipocyte","anti-inflammation","mangostin","Nrf2","obesity"],"languages":["English"],"rights":["unrestricted","This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://rave.ohiolink.edu/etdc/view?acc_num=osu1366099986","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Ziouzenkova, Ouliana"]},{"key":"dc:creator","label":"Author","values":["Shen, Qiwen"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2013-07-24"]},{"key":"dc:publisher","label":"Institution","values":["The Ohio State University / OhioLINK"]},{"key":"dc:type","label":"Dc Type","values":["Electronic Thesis or Dissertation"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Human Ecology: Human Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["The Ohio State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Nutrition","adipocyte","anti-inflammation","mangostin","Nrf2","obesity"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:rights","label":"Dc Rights","values":["unrestricted","This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://rave.ohiolink.edu/etdc/view?acc_num=osu1366099986"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["We developed three fluorescence biosensors based on a 3T3-L1 cell line that stably expressed nuclear factor κB response element (<i>Nfkb</i>-RE)/GFP, fatty acid binding protein 4 promoter (<i>Fabp4</i>-P)/CFP, and nuclear factor erythroid 2-related factor2 promoter (<i>Nrf2</i>-P)/YFP fluorescent reporters. We hypothesized that fluorescent signals directly correlated to inflammatory, adipogenic, and antioxidant status in adipocytes, respectively. Two polyphenolic xanthones, α-mangostin (α-MG) and γ-mangostin (γ-MG) from mangosteen fruit, have been reported to have anti-adipogenic and anti-inflammatory activities. We validated inflammatory and adipogenic properties of the major mangosteen xanthone α-MG using a <i>Nfkb</i>-RE/GFP biosensor assay, traditional ELISA, and semi-quantitative PCR approach. We also identified unique properties of γ-MG, a minor xanthone in mangosteen fruit. γ-MG suppressed adipogenesis and the anti-inflammatory cytokine adiponectin, but retained its ability to suppress the <i>Nfkb</i>-RE/GFP reporter and reduced the secretion of MCP-1 as compared to control adipocytes. To gain insight into mechanisms involved in the inhibition of adipogenesis, we compared the effects of α-MG on the activation of PPARγ and its target gene <i>Fabp4</i>, and Nrf2, an inducer of <i>Pparg</i> expression. The screening in <i>Nrf2</i>-P/YFP adipocytes suggested that α-MG effectively and dose-dependently reduced <i>Nrf2</i> promoter activation, whereas <i>Fabp4</i> promoter activation was not influenced. Thus, the antioxidant response of α-MG appears to be an early mechanism for the suppression of adipogenic and inflammatory effects in adipocytes. The combined application of high-throughput fluorescence biosensors can provide an effective platform for the identification of nutraceuticals and the mechanism of their actions in adipocytes."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf","p.42","1.47 MB"]},{"key":"dc:title","label":"Title","values":["Identification of Anti-inflammatory and Antioxidant Properties of MangostinXanthones in Adipocyte Reporter Assays"]}]}],"canonical_facts":{"dc:contributor":["Ziouzenkova, Ouliana"],"dc:creator":["Shen, Qiwen"],"dc:date":["2013-07-24"],"dc:description":["We developed three fluorescence biosensors based on a 3T3-L1 cell line that stably expressed nuclear factor κB response element (<i>Nfkb</i>-RE)/GFP, fatty acid binding protein 4 promoter (<i>Fabp4</i>-P)/CFP, and nuclear factor erythroid 2-related factor2 promoter (<i>Nrf2</i>-P)/YFP fluorescent reporters. We hypothesized that fluorescent signals directly correlated to inflammatory, adipogenic, and antioxidant status in adipocytes, respectively. Two polyphenolic xanthones, α-mangostin (α-MG) and γ-mangostin (γ-MG) from mangosteen fruit, have been reported to have anti-adipogenic and anti-inflammatory activities. We validated inflammatory and adipogenic properties of the major mangosteen xanthone α-MG using a <i>Nfkb</i>-RE/GFP biosensor assay, traditional ELISA, and semi-quantitative PCR approach. We also identified unique properties of γ-MG, a minor xanthone in mangosteen fruit. γ-MG suppressed adipogenesis and the anti-inflammatory cytokine adiponectin, but retained its ability to suppress the <i>Nfkb</i>-RE/GFP reporter and reduced the secretion of MCP-1 as compared to control adipocytes. To gain insight into mechanisms involved in the inhibition of adipogenesis, we compared the effects of α-MG on the activation of PPARγ and its target gene <i>Fabp4</i>, and Nrf2, an inducer of <i>Pparg</i> expression. The screening in <i>Nrf2</i>-P/YFP adipocytes suggested that α-MG effectively and dose-dependently reduced <i>Nrf2</i> promoter activation, whereas <i>Fabp4</i> promoter activation was not influenced. Thus, the antioxidant response of α-MG appears to be an early mechanism for the suppression of adipogenic and inflammatory effects in adipocytes. The combined application of high-throughput fluorescence biosensors can provide an effective platform for the identification of nutraceuticals and the mechanism of their actions in adipocytes."],"dc:format":["application/pdf","p.42","1.47 MB"],"dc:identifier":["http://rave.ohiolink.edu/etdc/view?acc_num=osu1366099986"],"dc:language":["English"],"dc:publisher":["The Ohio State University / OhioLINK"],"dc:rights":["unrestricted","This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws."],"dc:subject":["Nutrition","adipocyte","anti-inflammation","mangostin","Nrf2","obesity"],"dc:title":["Identification of Anti-inflammatory and Antioxidant Properties of MangostinXanthones in Adipocyte Reporter Assays"],"dc:type":["Electronic Thesis or Dissertation"],"thesis:degree_discipline":["Human Ecology: Human Nutrition"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["The Ohio State University"]},"updated_at":"2026-07-24T03:37:46Z"}