{"id":{"repo_id":"ohiolink","oai_identifier":"oai:etd.ohiolink.edu:mco1364857296"},"canonical_url":"https://search.dev.ndltd.org/etd/ohiolink/oai:etd.ohiolink.edu:mco1364857296","repository":{"repo_id":"ohiolink","name":"OhioLINK","base_url":"https://etd.ohiolink.edu/acprod/odb_etd/ws/oai/oai"},"display":{"title":"Identification and Characterization of A Zebrafish Glutathione S-Transferase Pi","abstract":"Glutathione S-transferases (GSTs) are a major group of Phase II enzymes involved in the detoxification of certain endogenous compounds such as reactive oxygen species (ROS) generated during metabolism, as well as in the detoxification of xenobiotics including drugs. In recent years, the zebrafish has increasingly been used as a promising animal model for biomedical research. This study is part of an effort to establish the zebrafish as a model for studying GST-mediated glutathione conjugation of xenobiotics. By searching the GenBank database, we have identified sequences encoding fifteen zebrafish GSTs. Using a computer algorithm available at the Genebee website, a dendrogram of the fifteen zebrafish GSTs was generated. A zebrafish GST Pi was subsequently cloned, expressed, and purified. An enzymatic assay for purified GST Pi was established using 1-chloro-2, 4-dinitrobenzene (CDNB) as a substrate. A pH-dependency study was performed, and the results indicated that the zebrafish GST Pi was active over a pH range of 6 and 8 with optimal activity observed at pH 7.5. The expression of novel zebrafishGST Pi during various developmental stages from embryogenesis to maturity was examined via reverse transcription-polymerase chain reaction (RT-PCR).","abstract_html":"Glutathione S-transferases (GSTs) are a major group of Phase II enzymes involved in the detoxification of certain endogenous compounds such as reactive oxygen species (ROS) generated during metabolism, as well as in the detoxification of xenobiotics including drugs. In recent years, the zebrafish has increasingly been used as a promising animal model for biomedical research. This study is part of an effort to establish the zebrafish as a model for studying GST-mediated glutathione conjugation of xenobiotics. By searching the GenBank database, we have identified sequences encoding fifteen zebrafish GSTs. Using a computer algorithm available at the Genebee website, a dendrogram of the fifteen zebrafish GSTs was generated. A zebrafish GST Pi was subsequently cloned, expressed, and purified. An enzymatic assay for purified GST Pi was established using 1-chloro-2, 4-dinitrobenzene (CDNB) as a substrate. A pH-dependency study was performed, and the results indicated that the zebrafish GST Pi was active over a pH range of 6 and 8 with optimal activity observed at pH 7.5. The expression of novel zebrafishGST Pi during various developmental stages from embryogenesis to maturity was examined via reverse transcription-polymerase chain reaction (RT-PCR).","abstract_has_math":false,"creators":["Abunnaja, Maryam"],"institution":"University of Toledo Health Science Campus","degree_name":"Master of Science in Pharmaceutical Science (MSP)","degree_level":"masters","degree_discipline":"College of Pharmacy","degree_department":null,"school":null,"contributors":["Liu, Ming-Cheh"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-05-20","date_published":"2013-05-20","updated_at":"2026-07-24T03:37:16Z","subjects":["Pharmacology"],"languages":["English"],"rights":["unrestricted","This thesis or dissertation is protected by copyright: all rights reserved. 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Using a computer algorithm available at the Genebee website, a dendrogram of the fifteen zebrafish GSTs was generated. A zebrafish GST Pi was subsequently cloned, expressed, and purified. An enzymatic assay for purified GST Pi was established using 1-chloro-2, 4-dinitrobenzene (CDNB) as a substrate. A pH-dependency study was performed, and the results indicated that the zebrafish GST Pi was active over a pH range of 6 and 8 with optimal activity observed at pH 7.5. The expression of novel zebrafishGST Pi during various developmental stages from embryogenesis to maturity was examined via reverse transcription-polymerase chain reaction (RT-PCR)."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf","p.38","666.1 KB"]},{"key":"dc:title","label":"Title","values":["Identification and Characterization of A Zebrafish Glutathione S-Transferase Pi"]}]}],"canonical_facts":{"dc:contributor":["Liu, Ming-Cheh"],"dc:creator":["Abunnaja, Maryam"],"dc:date":["2013-05-20"],"dc:description":["Glutathione S-transferases (GSTs) are a major group of Phase II enzymes involved in the detoxification of certain endogenous compounds such as reactive oxygen species (ROS) generated during metabolism, as well as in the detoxification of xenobiotics including drugs. In recent years, the zebrafish has increasingly been used as a promising animal model for biomedical research. 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