{"id":{"repo_id":"ohiolink","oai_identifier":"oai:etd.ohiolink.edu:mco1364331841"},"canonical_url":"https://search.dev.ndltd.org/etd/ohiolink/oai:etd.ohiolink.edu:mco1364331841","repository":{"repo_id":"ohiolink","name":"OhioLINK","base_url":"https://etd.ohiolink.edu/acprod/odb_etd/ws/oai/oai"},"display":{"title":"Role of Cofilin, an Actin Cytoskeletal Protein, in Ischemic Conditions: Potential Therapeutic Target for Ischemic Stroke","abstract":"Cerebral ischemia or stroke is a condition associated with decreased blood supply to brain leading to death of neurons. It is associated with a diverse cascade of responses involving both degenerative and regenerative mechanisms. Actin cytoskeleton changes play a key role in mediating these responses. Cofilin, an actin cytoskeletal severing protein is known to be involved in early stages of apoptotic cell death. Evidence supports its intervention in progression of disease states like Alzheimer’s and ischemic kidney disease. We have hypothesized possible involvement of cofilin in cerebral ischemia and neurodegeneration. To investigate the potential actin cytoskeletal changes induced by cofilin in ischemia, various ischemic models such as oxidative stress, oxygen glucose deprivation (OGD) in vitro and permanent middle cerebral artery occlusion (pMCAO) in vivo were used. The expression profile studies by immunoblotting and fluorescence methods displayed decrease in phosphocofilin (inactive form of cofilin) levels with all models of ischemia implying stress induced cofilin activation. Furthermore, calcineurin and slingshot 1L (SSH) phosphatases were found to be the signaling mediators of the cofilin activation pathway in ischemia elucidated by our inhibitor studies. These results strongly support cofilin role in cerebral ischemia induced actin cytoskeleton alterations leading to cell damage. We believe targeting this protein mediator has a potential for therapeutic intervention in stroke.","abstract_html":"Cerebral ischemia or stroke is a condition associated with decreased blood supply to brain leading to death of neurons. It is associated with a diverse cascade of responses involving both degenerative and regenerative mechanisms. Actin cytoskeleton changes play a key role in mediating these responses. Cofilin, an actin cytoskeletal severing protein is known to be involved in early stages of apoptotic cell death. Evidence supports its intervention in progression of disease states like Alzheimer’s and ischemic kidney disease. We have hypothesized possible involvement of cofilin in cerebral ischemia and neurodegeneration. To investigate the potential actin cytoskeletal changes induced by cofilin in ischemia, various ischemic models such as oxidative stress, oxygen glucose deprivation (OGD) in vitro and permanent middle cerebral artery occlusion (pMCAO) in vivo were used. The expression profile studies by immunoblotting and fluorescence methods displayed decrease in phosphocofilin (inactive form of cofilin) levels with all models of ischemia implying stress induced cofilin activation. Furthermore, calcineurin and slingshot 1L (SSH) phosphatases were found to be the signaling mediators of the cofilin activation pathway in ischemia elucidated by our inhibitor studies. These results strongly support cofilin role in cerebral ischemia induced actin cytoskeleton alterations leading to cell damage. We believe targeting this protein mediator has a potential for therapeutic intervention in stroke.","abstract_has_math":false,"creators":["Madineni, Anusha"],"institution":"University of Toledo Health Science Campus","degree_name":"Master of Science in Medicinal Chemistry (MSP)","degree_level":"masters","degree_discipline":"College of Pharmacy","degree_department":null,"school":null,"contributors":["Shah, Zahoor"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-05-20","date_published":"2013-05-20","updated_at":"2026-07-24T03:37:01Z","subjects":["Cellular Biology","Neurosciences","Pharmacology","Pharmaceuticals","Cofilin","Neurodegeneration","Cerebral ischemia"],"languages":["English"],"rights":["unrestricted","This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://rave.ohiolink.edu/etdc/view?acc_num=mco1364331841","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Shah, Zahoor"]},{"key":"dc:creator","label":"Author","values":["Madineni, Anusha"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2013-05-20"]},{"key":"dc:publisher","label":"Institution","values":["University of Toledo Health Science Campus / OhioLINK"]},{"key":"dc:type","label":"Dc Type","values":["Electronic Thesis or Dissertation"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["College of Pharmacy"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science in Medicinal Chemistry (MSP)"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Toledo Health Science Campus"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Cellular Biology","Neurosciences","Pharmacology","Pharmaceuticals","Cofilin","Neurodegeneration","Cerebral ischemia"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:rights","label":"Dc Rights","values":["unrestricted","This thesis or dissertation is protected by copyright: all rights reserved. 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We have hypothesized possible involvement of cofilin in cerebral ischemia and neurodegeneration. To investigate the potential actin cytoskeletal changes induced by cofilin in ischemia, various ischemic models such as oxidative stress, oxygen glucose deprivation (OGD) in vitro and permanent middle cerebral artery occlusion (pMCAO) in vivo were used. The expression profile studies by immunoblotting and fluorescence methods displayed decrease in phosphocofilin (inactive form of cofilin) levels with all models of ischemia implying stress induced cofilin activation. Furthermore, calcineurin and slingshot 1L (SSH) phosphatases were found to be the signaling mediators of the cofilin activation pathway in ischemia elucidated by our inhibitor studies. These results strongly support cofilin role in cerebral ischemia induced actin cytoskeleton alterations leading to cell damage. We believe targeting this protein mediator has a potential for therapeutic intervention in stroke."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf","p.62","1.05 MB"]},{"key":"dc:title","label":"Title","values":["Role of Cofilin, an Actin Cytoskeletal Protein, in Ischemic Conditions: Potential Therapeutic Target for Ischemic Stroke"]}]}],"canonical_facts":{"dc:contributor":["Shah, Zahoor"],"dc:creator":["Madineni, Anusha"],"dc:date":["2013-05-20"],"dc:description":["Cerebral ischemia or stroke is a condition associated with decreased blood supply to brain leading to death of neurons. It is associated with a diverse cascade of responses involving both degenerative and regenerative mechanisms. Actin cytoskeleton changes play a key role in mediating these responses. Cofilin, an actin cytoskeletal severing protein is known to be involved in early stages of apoptotic cell death. Evidence supports its intervention in progression of disease states like Alzheimer’s and ischemic kidney disease. We have hypothesized possible involvement of cofilin in cerebral ischemia and neurodegeneration. To investigate the potential actin cytoskeletal changes induced by cofilin in ischemia, various ischemic models such as oxidative stress, oxygen glucose deprivation (OGD) in vitro and permanent middle cerebral artery occlusion (pMCAO) in vivo were used. The expression profile studies by immunoblotting and fluorescence methods displayed decrease in phosphocofilin (inactive form of cofilin) levels with all models of ischemia implying stress induced cofilin activation. Furthermore, calcineurin and slingshot 1L (SSH) phosphatases were found to be the signaling mediators of the cofilin activation pathway in ischemia elucidated by our inhibitor studies. These results strongly support cofilin role in cerebral ischemia induced actin cytoskeleton alterations leading to cell damage. We believe targeting this protein mediator has a potential for therapeutic intervention in stroke."],"dc:format":["application/pdf","p.62","1.05 MB"],"dc:identifier":["http://rave.ohiolink.edu/etdc/view?acc_num=mco1364331841"],"dc:language":["English"],"dc:publisher":["University of Toledo Health Science Campus / OhioLINK"],"dc:rights":["unrestricted","This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws."],"dc:subject":["Cellular Biology","Neurosciences","Pharmacology","Pharmaceuticals","Cofilin","Neurodegeneration","Cerebral ischemia"],"dc:title":["Role of Cofilin, an Actin Cytoskeletal Protein, in Ischemic Conditions: Potential Therapeutic Target for Ischemic Stroke"],"dc:type":["Electronic Thesis or Dissertation"],"thesis:degree_discipline":["College of Pharmacy"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science in Medicinal Chemistry (MSP)"],"thesis:institution_name":["University of Toledo Health Science Campus"]},"updated_at":"2026-07-24T03:37:01Z"}