{"id":{"repo_id":"ohiolink","oai_identifier":"oai:etd.ohiolink.edu:akron1366197780"},"canonical_url":"https://search.dev.ndltd.org/etd/ohiolink/oai:etd.ohiolink.edu:akron1366197780","repository":{"repo_id":"ohiolink","name":"OhioLINK","base_url":"https://etd.ohiolink.edu/acprod/odb_etd/ws/oai/oai"},"display":{"title":"Preparation and Biocompatibility of Electrospun Zwitterionic Poly(Sulfobetaine Methacrylate) for Wound Dressing Applications","abstract":"Poly(sulfobetaine methacrylate) (PSBMA) can be potentially utilized in filtration and wound dressing applications for which nanofibers structures are highly desirable. In this work, a series of PSBMAs with different molecular weights were synthesized, characterized, and electrospun into nanofibers. The polymer molecular weight was controlled by varying the amount of redox initiators in the free radical polymerization of SBMA, with the highest molecular weight achieved at an intermediate initiator concentration. From the intrinsic viscosity measurements, the Marke-Houwink parameters for PSBMA (at 21°C in 0.2 M NaCl solution) was determined as a=0.4071 and k=2.06 x 10-3. Thermogravimetric (TGA) analysis shows that the PSBMAs were thermally stable up to at least 250°C. Fourier transform infrared (FTIR) spectra indicate major structural changes of both polymer backbone and pendant groups by thermal degradation. Results from differential scanning calorimetry (DSC), TGA, and FTIR characterizations all demonstrate the existence of water strongly bound in PSBMA. DSC analysis also indicates different degrees of crystallinity for the PSBMAs of different sizes. Viscosity of the PSBMA solutions, a critical parameter for electrospinning, increased with the solution concentration and the polymer molecular weight. For the electrospinning of PSBMA, it was found that high solution concentration and high molecular weight favored the formation of smooth fibers while low solution concentration or low molecular weight led to the formation of beaded fibers or beads. Fiber diameters ranging from 200 to 570nm were achieved by controlling solution concentration and polymer molecular weight. The characterization data and electrospinning results were finally correlated to explore the relationships between fiber formation, viscosity, molecular weight, and concentration.Zwitterionic PSBMA has been well studied for its superhydrophilic and ultralow biofouling properties, making it a promising material for superabsorbent and nonadherent wound dressings. Electrospinning provides multiple desirable features for wound dressings, including high absorptivity due to high surface-area-to-volume ratio, high gas permeation, and conformability to contour of the wound bed. The goal of this work is to develop a fibrous membrane of PSBMA via electrospinning and evaluate its properties related to wound dressing applications. Being superhydrophilic, PSBMA fibers fabricated by a conventional electrospinning method would readily dissolve in water, whereas if crosslinker is added, the formation of hydrogel would prevent electrospinning. A three step polymerization-electrospinning-photo-cross-linking process was developed in this work to fabricate the cross-linked electrospun PSBMA fibrous membrane. Such electrospun membrane was stable in water and exhibited high water absorption of 353%(w/w), whereas the PSBMA hydrogel only absorbed 81% water. The electrospun membrane showed strong resistance to protein adsorption and cell attachment. Bacterial adhesion studies using Gram negative P. aeruginosa and Gram positive S. epidermidis showed that the PSBMA electrospun membrane was also highly resistant to bacterial adhesion. The Ag+-impregnated electrospun PSBMA membrane was shown microbicidal, against both S. epidermidis and P. aeruginosa. Such electrospun PSBMA membrane is ideal for a novel type of nonadherent, superabsorbent, and antimicrobial wound dressing. The superior water absorption aids in fluid removal from highly exudating wounds while keeping the wound hydrated to support healing. Because of the resistance to protein, cell, and bacterial adhesion, the dressing removal will neither cause patients’ pain nor disturb the newly formed tissues. The dressing also prevents the attachment of environmental bacteria and offers broad spectrum antimicrobial activity. It is the first work to develop the water-stable electrospun PSBMA membrane, which has great potential for wound dressing and other applications.","abstract_html":"Poly(sulfobetaine methacrylate) (PSBMA) can be potentially utilized in filtration and wound dressing applications for which nanofibers structures are highly desirable. In this work, a series of PSBMAs with different molecular weights were synthesized, characterized, and electrospun into nanofibers. The polymer molecular weight was controlled by varying the amount of redox initiators in the free radical polymerization of SBMA, with the highest molecular weight achieved at an intermediate initiator concentration. From the intrinsic viscosity measurements, the Marke-Houwink parameters for PSBMA (at 21°C in 0.2 M NaCl solution) was determined as a=0.4071 and k=2.06 x 10-3. Thermogravimetric (TGA) analysis shows that the PSBMAs were thermally stable up to at least 250°C. Fourier transform infrared (FTIR) spectra indicate major structural changes of both polymer backbone and pendant groups by thermal degradation. Results from differential scanning calorimetry (DSC), TGA, and FTIR characterizations all demonstrate the existence of water strongly bound in PSBMA. DSC analysis also indicates different degrees of crystallinity for the PSBMAs of different sizes. Viscosity of the PSBMA solutions, a critical parameter for electrospinning, increased with the solution concentration and the polymer molecular weight. For the electrospinning of PSBMA, it was found that high solution concentration and high molecular weight favored the formation of smooth fibers while low solution concentration or low molecular weight led to the formation of beaded fibers or beads. Fiber diameters ranging from 200 to 570nm were achieved by controlling solution concentration and polymer molecular weight. The characterization data and electrospinning results were finally correlated to explore the relationships between fiber formation, viscosity, molecular weight, and concentration.Zwitterionic PSBMA has been well studied for its superhydrophilic and ultralow biofouling properties, making it a promising material for superabsorbent and nonadherent wound dressings. Electrospinning provides multiple desirable features for wound dressings, including high absorptivity due to high surface-area-to-volume ratio, high gas permeation, and conformability to contour of the wound bed. The goal of this work is to develop a fibrous membrane of PSBMA via electrospinning and evaluate its properties related to wound dressing applications. Being superhydrophilic, PSBMA fibers fabricated by a conventional electrospinning method would readily dissolve in water, whereas if crosslinker is added, the formation of hydrogel would prevent electrospinning. A three step polymerization-electrospinning-photo-cross-linking process was developed in this work to fabricate the cross-linked electrospun PSBMA fibrous membrane. Such electrospun membrane was stable in water and exhibited high water absorption of 353%(w/w), whereas the PSBMA hydrogel only absorbed 81% water. The electrospun membrane showed strong resistance to protein adsorption and cell attachment. Bacterial adhesion studies using Gram negative P. aeruginosa and Gram positive S. epidermidis showed that the PSBMA electrospun membrane was also highly resistant to bacterial adhesion. The Ag+-impregnated electrospun PSBMA membrane was shown microbicidal, against both S. epidermidis and P. aeruginosa. Such electrospun PSBMA membrane is ideal for a novel type of nonadherent, superabsorbent, and antimicrobial wound dressing. The superior water absorption aids in fluid removal from highly exudating wounds while keeping the wound hydrated to support healing. Because of the resistance to protein, cell, and bacterial adhesion, the dressing removal will neither cause patients’ pain nor disturb the newly formed tissues. The dressing also prevents the attachment of environmental bacteria and offers broad spectrum antimicrobial activity. It is the first work to develop the water-stable electrospun PSBMA membrane, which has great potential for wound dressing and other applications.","abstract_has_math":false,"creators":["Lalani, Reza"],"institution":"University of Akron","degree_name":"Doctor of Philosophy","degree_level":"doctoral","degree_discipline":"Integrated Bioscience","degree_department":null,"school":null,"contributors":["Liu, Lingyun"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-05-09","date_published":"2013-05-09","updated_at":"2026-07-24T03:37:46Z","subjects":["Biology","Chemical Engineering","Chemistry","Polymers","Polymer Chemistry","hydrophilic polysulfobetaine","electrospinning","wound dressing"],"languages":["English"],"rights":["unrestricted","This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://rave.ohiolink.edu/etdc/view?acc_num=akron1366197780","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Liu, Lingyun"]},{"key":"dc:creator","label":"Author","values":["Lalani, Reza"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2013-05-09"]},{"key":"dc:publisher","label":"Institution","values":["University of Akron / OhioLINK"]},{"key":"dc:type","label":"Dc Type","values":["Electronic Thesis or Dissertation"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Integrated Bioscience"]},{"key":"thesis:degree_level","label":"Degree Level","values":["doctoral"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Akron"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology","Chemical Engineering","Chemistry","Polymers","Polymer Chemistry","hydrophilic polysulfobetaine","electrospinning","wound dressing"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:rights","label":"Dc Rights","values":["unrestricted","This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://rave.ohiolink.edu/etdc/view?acc_num=akron1366197780"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Poly(sulfobetaine methacrylate) (PSBMA) can be potentially utilized in filtration and wound dressing applications for which nanofibers structures are highly desirable. In this work, a series of PSBMAs with different molecular weights were synthesized, characterized, and electrospun into nanofibers. The polymer molecular weight was controlled by varying the amount of redox initiators in the free radical polymerization of SBMA, with the highest molecular weight achieved at an intermediate initiator concentration. From the intrinsic viscosity measurements, the Marke-Houwink parameters for PSBMA (at 21°C in 0.2 M NaCl solution) was determined as a=0.4071 and k=2.06 x 10-3. Thermogravimetric (TGA) analysis shows that the PSBMAs were thermally stable up to at least 250°C. Fourier transform infrared (FTIR) spectra indicate major structural changes of both polymer backbone and pendant groups by thermal degradation. Results from differential scanning calorimetry (DSC), TGA, and FTIR characterizations all demonstrate the existence of water strongly bound in PSBMA. DSC analysis also indicates different degrees of crystallinity for the PSBMAs of different sizes. Viscosity of the PSBMA solutions, a critical parameter for electrospinning, increased with the solution concentration and the polymer molecular weight. For the electrospinning of PSBMA, it was found that high solution concentration and high molecular weight favored the formation of smooth fibers while low solution concentration or low molecular weight led to the formation of beaded fibers or beads. Fiber diameters ranging from 200 to 570nm were achieved by controlling solution concentration and polymer molecular weight. The characterization data and electrospinning results were finally correlated to explore the relationships between fiber formation, viscosity, molecular weight, and concentration.Zwitterionic PSBMA has been well studied for its superhydrophilic and ultralow biofouling properties, making it a promising material for superabsorbent and nonadherent wound dressings. Electrospinning provides multiple desirable features for wound dressings, including high absorptivity due to high surface-area-to-volume ratio, high gas permeation, and conformability to contour of the wound bed. The goal of this work is to develop a fibrous membrane of PSBMA via electrospinning and evaluate its properties related to wound dressing applications. Being superhydrophilic, PSBMA fibers fabricated by a conventional electrospinning method would readily dissolve in water, whereas if crosslinker is added, the formation of hydrogel would prevent electrospinning. A three step polymerization-electrospinning-photo-cross-linking process was developed in this work to fabricate the cross-linked electrospun PSBMA fibrous membrane. Such electrospun membrane was stable in water and exhibited high water absorption of 353%(w/w), whereas the PSBMA hydrogel only absorbed 81% water. The electrospun membrane showed strong resistance to protein adsorption and cell attachment. Bacterial adhesion studies using Gram negative P. aeruginosa and Gram positive S. epidermidis showed that the PSBMA electrospun membrane was also highly resistant to bacterial adhesion. The Ag+-impregnated electrospun PSBMA membrane was shown microbicidal, against both S. epidermidis and P. aeruginosa. Such electrospun PSBMA membrane is ideal for a novel type of nonadherent, superabsorbent, and antimicrobial wound dressing. The superior water absorption aids in fluid removal from highly exudating wounds while keeping the wound hydrated to support healing. Because of the resistance to protein, cell, and bacterial adhesion, the dressing removal will neither cause patients’ pain nor disturb the newly formed tissues. The dressing also prevents the attachment of environmental bacteria and offers broad spectrum antimicrobial activity. It is the first work to develop the water-stable electrospun PSBMA membrane, which has great potential for wound dressing and other applications."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf","p.120","7.94 MB"]},{"key":"dc:title","label":"Title","values":["Preparation and Biocompatibility of Electrospun Zwitterionic Poly(Sulfobetaine Methacrylate) for Wound Dressing Applications"]}]}],"canonical_facts":{"dc:contributor":["Liu, Lingyun"],"dc:creator":["Lalani, Reza"],"dc:date":["2013-05-09"],"dc:description":["Poly(sulfobetaine methacrylate) (PSBMA) can be potentially utilized in filtration and wound dressing applications for which nanofibers structures are highly desirable. In this work, a series of PSBMAs with different molecular weights were synthesized, characterized, and electrospun into nanofibers. The polymer molecular weight was controlled by varying the amount of redox initiators in the free radical polymerization of SBMA, with the highest molecular weight achieved at an intermediate initiator concentration. From the intrinsic viscosity measurements, the Marke-Houwink parameters for PSBMA (at 21°C in 0.2 M NaCl solution) was determined as a=0.4071 and k=2.06 x 10-3. Thermogravimetric (TGA) analysis shows that the PSBMAs were thermally stable up to at least 250°C. Fourier transform infrared (FTIR) spectra indicate major structural changes of both polymer backbone and pendant groups by thermal degradation. Results from differential scanning calorimetry (DSC), TGA, and FTIR characterizations all demonstrate the existence of water strongly bound in PSBMA. DSC analysis also indicates different degrees of crystallinity for the PSBMAs of different sizes. Viscosity of the PSBMA solutions, a critical parameter for electrospinning, increased with the solution concentration and the polymer molecular weight. For the electrospinning of PSBMA, it was found that high solution concentration and high molecular weight favored the formation of smooth fibers while low solution concentration or low molecular weight led to the formation of beaded fibers or beads. Fiber diameters ranging from 200 to 570nm were achieved by controlling solution concentration and polymer molecular weight. The characterization data and electrospinning results were finally correlated to explore the relationships between fiber formation, viscosity, molecular weight, and concentration.Zwitterionic PSBMA has been well studied for its superhydrophilic and ultralow biofouling properties, making it a promising material for superabsorbent and nonadherent wound dressings. Electrospinning provides multiple desirable features for wound dressings, including high absorptivity due to high surface-area-to-volume ratio, high gas permeation, and conformability to contour of the wound bed. The goal of this work is to develop a fibrous membrane of PSBMA via electrospinning and evaluate its properties related to wound dressing applications. Being superhydrophilic, PSBMA fibers fabricated by a conventional electrospinning method would readily dissolve in water, whereas if crosslinker is added, the formation of hydrogel would prevent electrospinning. A three step polymerization-electrospinning-photo-cross-linking process was developed in this work to fabricate the cross-linked electrospun PSBMA fibrous membrane. Such electrospun membrane was stable in water and exhibited high water absorption of 353%(w/w), whereas the PSBMA hydrogel only absorbed 81% water. The electrospun membrane showed strong resistance to protein adsorption and cell attachment. Bacterial adhesion studies using Gram negative P. aeruginosa and Gram positive S. epidermidis showed that the PSBMA electrospun membrane was also highly resistant to bacterial adhesion. The Ag+-impregnated electrospun PSBMA membrane was shown microbicidal, against both S. epidermidis and P. aeruginosa. Such electrospun PSBMA membrane is ideal for a novel type of nonadherent, superabsorbent, and antimicrobial wound dressing. The superior water absorption aids in fluid removal from highly exudating wounds while keeping the wound hydrated to support healing. Because of the resistance to protein, cell, and bacterial adhesion, the dressing removal will neither cause patients’ pain nor disturb the newly formed tissues. The dressing also prevents the attachment of environmental bacteria and offers broad spectrum antimicrobial activity. It is the first work to develop the water-stable electrospun PSBMA membrane, which has great potential for wound dressing and other applications."],"dc:format":["application/pdf","p.120","7.94 MB"],"dc:identifier":["http://rave.ohiolink.edu/etdc/view?acc_num=akron1366197780"],"dc:language":["English"],"dc:publisher":["University of Akron / OhioLINK"],"dc:rights":["unrestricted","This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws."],"dc:subject":["Biology","Chemical Engineering","Chemistry","Polymers","Polymer Chemistry","hydrophilic polysulfobetaine","electrospinning","wound dressing"],"dc:title":["Preparation and Biocompatibility of Electrospun Zwitterionic Poly(Sulfobetaine Methacrylate) for Wound Dressing Applications"],"dc:type":["Electronic Thesis or Dissertation"],"thesis:degree_discipline":["Integrated Bioscience"],"thesis:degree_level":["doctoral"],"thesis:degree_name":["Doctor of Philosophy"],"thesis:institution_name":["University of Akron"]},"updated_at":"2026-07-24T03:37:46Z"}