In the Margins: Reconsidering the Range and Contribution of Diazotrophs in Nearshore Environments

<p>Dinitrogen (N₂) fixation enables primary production and, consequently, carbon dioxide drawdown in nitrogen (N) limited marine systems, exerting a powerful influence over the coupled carbon and N cycles. Our understanding of the environmental factors regulating its distribution and magnitude are largely based on the range and sensitivity of one genus, T<em>richodesmium</em>. However, recent work suggests that the niche preferences of distinct diazotrophic (N₂ fixing) clades differ due to their metabolic and ecological diversity, hampering efforts to close the N budget and model N₂ fixation accurately. Here, I explore the range of N₂ fixation across physico-chemical gradients (e.g., light, nutrients, oxygen) in nearshore environments of significance in global biogeochemical cycling: the major pelagic oxygen deficient zones (ODZs) in the Eastern Tropical South (ETSP) and North (ETNP) Pacific Ocean, and the broad continental shelf of the Western North Atlantic Ocean (WNA). The ODZs are hypothesized to play an important role in N cycle homeostasis by generating conditions thought to promote diazotrophy; recent work suggests that broad continental shelf environments may contribute substantially to new reactive N inputs globally. N₂ fixation rates were measured using a robust ¹⁵N tracer method that accounts for the slow dissolution of N2 gas. To explore niche partitioning and better characterize spatial heterogeneity on the WNA shelf, I built an empirical model of N₂ fixation and investigated diazotroph identity using amplicon sequencing and qPCR. In the ETSP, N₂ fixation was only detected in a subset of low-oxygen samples. N₂ fixation within the ETNP ODZ was patchy and driven by organic carbon availability; however, significant rates were observed at coastal stations near the Gulf of California. Frontal mixing on the WNA shelf resulted in exceptionally high rates of N₂ fixation, associated with high UCYN-A activity. My findings suggest that (1) diazotrophy is more energetically favorable (relative to dissolved inorganic N) in low-oxygen waters but may be carbon-limited, and (2) continental inputs and dynamic conditions at coastal margins can favor significant N inputs via diazotrophy.</p>

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Ocean & Earth Sciences

Year dc:date.available

2020

Author dc:creator

• Selden, Corday R.

Contributors dc:contributor

• Margaret R. Mulholland
• P. Dreux Chappell
• Angela Knapp
• John Whiteman

• Continental shelf
• Diazotroph
• Frontal zone
• Machine learning
• Nitrogen
• Oxygen deficient zone
• Biogeochemistry
• Microbiology
• Oceanography

Statement dc:rights

• <p>In Copyright. URI: <a href="http://rightsstatements.org/vocab/InC/1.0/">http://rightsstatements.org/vocab/InC/1.0/</a> This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).</p>

Identifier

9798557051835

OAI identifier oai:identifier

oai:digitalcommons.odu.edu:oeas_etds-1174