Platelet Function and Energy Metabolism During Storage in a Well-Defined Experimental Medium

<p>The most significant problem in maintaining platelet viability during storage is the decrease in pH resulting from accumulation of lactic acid formed by platelet anaerobic glycolysis. This study investigates nutrient alternatives to glucose for maintenance of morphology and functional integrity during platelet storage in a well-defined synthetic medium and correlates this data with purine nucleotide levels determined by high-performance liquid chromatography (HPLC) and other in vitro measures of platelet quality. Platelets prepared from pooled ABO- and Rh- identical platelet-rich plasma were stored as concentrates in the synthetic medium without added nutrients (control) and with added substrates.</p> <p>The HPLC procedure developed for this study permits simultaneous analysis of purine nucleotides, nucleosides, and bases. The separation is performed on a C₁₈ column (end-capped) using 0.1 M phosphate buffer, pH 5.4, containing dibutylamine phosphate (D-4) ion pair reagent and a non-linear, concave gradient to 0.1 M phosphate buffer, pH 5.4, with D-4 reagent in water:acetonitrile (75:25, v/v). The results for metabolic and storage nucleotide pools on day 1 of storage were consistent with previously reported levels in platelets</p>

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry and Biochemistry

Year dc:date.available

1989

Author dc:creator

• Keegan, Thais Edwina Ahrendt

Contributors dc:contributor

• Patricia Pleban
• Andrew Heaton
• Wiliam Cooke
• Stein Holme
• John Van Norman

• Platelets
• Energy metabolism
• Storage
• Biochemistry

Repository record dc:identifier

https://digitalcommons.odu.edu/biomedicalsciences_etds/112

OAI identifier oai:identifier

oai:digitalcommons.odu.edu:biomedicalsciences_etds-1120