The Assessment of Osteoinductivity of Human Allograft Demineralized Bone Matrix by In Vivo and In Vitro Assay Models
type: CreativeWork
description: The osteoinductive potential (osteoinductivity) is a very important aspect of demineralized bone matrix (DBM) as a biomaterial. This study is directed at establishing qualitative and quantitative assays of the osteoinductivity of human DBM. Further aspects of this study include an assessment of the effects of the production process, matrix characterization, and donor related factors on osteoinductivity of the DBM. Athymic mice were chosen as the animal model for use in the in vivo bioassay. Implantation of DBM in muscle pouches provided for much higher osteoinductivity (remineralization to 10% calcium) than subcutaneous site implantation (remineralization to 1.6% calcium). Histological observations revealed that new bone and bone marrow formed after four weeks of intramuscular implantation. In the in vivo dose-response studies, 20 mg of DBM provided for the highest levels of osteoinductivity. Human periosteal cells (HPO/CB-MZ01) were chosen for the in vitro bioassays of the osteoinductivity of DBM. Both biochemical and histochemical studies revealed that the DBM can induce significantly higher alkaline phosphatase (ALP) activities when compared to DBM-free control flasks after four days of incubation. In the in vitro dose-response studies 5 and 10 mg of DBM produced the highest osteoinductivity. The study of the effect(s) of residual calcium on osteoinductivity revealed that nondemineralized bone matrix lacked significant osteoinductivity. As the residual calcium content decreased, the degree of remineralization produced by the bone matrix increased, reaching maximum remineralization when the residual calcium content of bone matrix approached 2%. At residual calcium levels below 2%, osteoinductivity of the DBM decreased. The study of the effect of particle size revealed that DBM particles less than 250 micron produced the lowest osteoinductivity among all size groups studied. Bone particles in the 500-710 micron size range provided for DBM with the highest osteoindu…
abstract: The osteoinductive potential (osteoinductivity) is a very important aspect of demineralized bone matrix (DBM) as a biomaterial. This study is directed at establishing qualitative and quantitative assays of the osteoinductivity of human DBM. Further aspects of this study include an assessment of the effects of the production process, matrix characterization, and donor related factors on osteoinductivity of the DBM. Athymic mice were chosen as the animal model for use in the in vivo bioassay. Implantation of DBM in muscle pouches provided for much higher osteoinductivity (remineralization to 10% calcium) than subcutaneous site implantation (remineralization to 1.6% calcium). Histological observations revealed that new bone and bone marrow formed after four weeks of intramuscular implantation. In the in vivo dose-response studies, 20 mg of DBM provided for the highest levels of osteoinductivity. Human periosteal cells (HPO/CB-MZ01) were chosen for the in vitro bioassays of the osteoinductivity of DBM. Both biochemical and histochemical studies revealed that the DBM can induce significantly higher alkaline phosphatase (ALP) activities when compared to DBM-free control flasks after four days of incubation. In the in vitro dose-response studies 5 and 10 mg of DBM produced the highest osteoinductivity. The study of the effect(s) of residual calcium on osteoinductivity revealed that nondemineralized bone matrix lacked significant osteoinductivity. As the residual calcium content decreased, the degree of remineralization produced by the bone matrix increased, reaching maximum remineralization when the residual calcium content of bone matrix approached 2%. At residual calcium levels below 2%, osteoinductivity of the DBM decreased. The study of the effect of particle size revealed that DBM particles less than 250 micron produced the lowest osteoinductivity among all size groups studied. Bone particles in the 500-710 micron size range provided for DBM with the highest osteoindu…
author: Zhang, Min
keywords: Assay models, Osteoinductive potential, Demineralized bone matrix, Anatomy, Biophysics, Physiology
date published: 1996-04-01T08:00:00Z
date modified: 2026-07-24T03:34:46Z
provider: Old Dominion University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Old Dominion University