Characterization of the Fast Axonally Transported Proteins in the Rat Optic Pathway

<p>The fast axonally transported proteins represent a subset of neuronal proteins that are conveyed anterogradely as secretory vesicle constituents from the perikarya. Although fast transport displays properties consistent with the general secretory pathway, neuronal structure presents special problems which may require modifications of the general pathway. The elucidation of these special modifications is essential for a more complete understanding of neuronal function both in normal and pathological conditions. In order to characterize the fast transported (FT) proteins and to better understand fast transport trafficking, the FT of radiolabeled retinal ganglion cell (RGC) proteins in the adult rat optic pathway was studied. The optic pathway model system afforded the opportunity to delineate FT trafficking in the axon from FT trafficking at pre-synaptic terminals. Radiolabel studies of FT proteins revealed six distinct classes of proteins. The first class of FT proteins were found to have little or no trafficking preference for axons or pre-synaptic terminals. The second class of FT proteins exhibited no compartmental trafficking preference and decreased in specific activity from 4-48 hr post-labeling. Such decrease could suggest rapid turnover of these FT proteins. A third class of FT proteins showed increased specific activity only in the axon compartment at 24 hour post intra-ocular labeling (PIL). A fourth class of FT proteins appeared to be trafficked to both compartments, but with a slower time course than the general FT population. The fifth and sixth classes of proteins represent those proteins that were preferentially localized to the terminal and axon compartments, respectively. Fractionation of synaptosomal membranes revealed that the majority, but not all, of FT proteins were conveyed as integral membrane proteins. Immunoprecipitation revealed the major ³⁵S-methionine labeled protein in the rat optic pathway (SNAP-25) was found to be associated with specific subsets of FT and slow transported (SCb) proteins. In summary, the FT proteins in the rat optic pathway displayed heterogenous transport initiation, kinetics, compartmentation, and intermolecular association. Since axons lack sorting organelles (e.g., Golgi), these results suggest that neurons have evolved complex mechanisms for differential delivery of FT proteins from the perikarya.</p>

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Dissertation

Year dc:date.available

1997

Author dc:creator

• Mulugeta, Surafel

Contributors dc:contributor

• Bruce W. Tedeschi
• Paul F. Aravich
• Keith A. Carson
• Francis J. liuzzi
• Charles W. Morgan

• Axonal transport
• Fast proteins
• Protein trafficking
• Neurology

Identifier

9780591603927

OAI identifier oai:identifier

oai:digitalcommons.odu.edu:biomedicalsciences_etds-1064