Detection of Unique Tumor-Associated Proteins in the Urine of Patients with Transitional Cell Carcinoma by High Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis

<p>A two-dimensional polyacrylamide gel electrophoretic technique was developed for the detection of unique tumor-associated proteins in urine of patients with transitional cell carcinoma (TCC) of the urinary bladder. This technique yielded high resolution of polypeptides in the urine of cancer patients. Urine was chosen as a source of tumor-associated components because the location of the tum or should release already solublized tum or substances in to the urine.</p> <p>Urinary proteins were concentrated and then analyzed by the two - dimensional electrophoretic technique using isoelectric focusing in the first dimension and sodium dodecylsulfate - polyacrylamide gel electrophoresis in the second dimension. Ten transitional cell carcinoma patients, ten renal cell carcinoma patients, two benign bladder patients, two benign kidney patients, and seven normal donors were included in this study. Urinary proteins that were associated with transitional cell carcinoma and not found in normal urine or urine from patients with benign diseases are described.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biological Sciences

Year dc:date.available

1979

Author dc:creator

• Lehman, Robert David

Contributors dc:contributor

• Edgar F. Stillwell
• George L. Wright, Jr.
• Lloyd Wolfinbarger

• Tumor proteins
• Urine analysis
• Gel
• Carcinoma
• Cancer Biology
• Cell Biology
• Immunology and Infectious Disease
• Oncology

Statement dc:rights

• <p>In Copyright. URI: <a href="http://rightsstatements.org/vocab/InC/1.0/">http://rightsstatements.org/vocab/InC/1.0/</a> This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).</p>

Repository record dc:identifier

https://digitalcommons.odu.edu/biology_etds/88

OAI identifier oai:identifier

oai:digitalcommons.odu.edu:biology_etds-1088