ANALYSIS OF TWO NOVEL COMPLEXES THAT REGULATE NEUROBLAST SELF-RENEWAL AND ASYMMETRIC DIVISION IN DROSOPHILA MELANOGASTER
type: CreativeWork
description: Drosophila neuroblast divides asymmetrically to produce a self-renewing neuroblast and a ganglion mother cell that is committed to differentiation. The balance between neuroblast self-renewal and differentiation is tightly controlled to ensure the proper development of the central nervous system. In this thesis, I will describe two novel players that regulate Drosophila neuroblast asymmetric cell division. The first one is a multi-protein ubiquitin ligase, SCF-Slimb that is composed of SkpA, Cullin1, Roc1a and Slimb. The SCF-Slimb complex inhibits neuroblast self-renewal by regulating the polarized localization of atypical protein kinase C (aPKC) and cell fate determinant Numb, as well as mitotic spindle orientation in neuroblasts. The other player is Drosophila cytoplasmic dynein light chain 1, also named Cut up (Ctp). Ctp localizes at centrioles and controls the mitotic spindle orientation in neuroblasts. Ctp directly interacts with a centriolar protein Anastral Spindle 2 (Ana2) and regulates the localization of Mushroom body defect (Mud). Ana2, Ctp and Mud form a protein complex to regulate spindle orientation in neuroblasts.
abstract: Drosophila neuroblast divides asymmetrically to produce a self-renewing neuroblast and a ganglion mother cell that is committed to differentiation. The balance between neuroblast self-renewal and differentiation is tightly controlled to ensure the proper development of the central nervous system. In this thesis, I will describe two novel players that regulate Drosophila neuroblast asymmetric cell division. The first one is a multi-protein ubiquitin ligase, SCF-Slimb that is composed of SkpA, Cullin1, Roc1a and Slimb. The SCF-Slimb complex inhibits neuroblast self-renewal by regulating the polarized localization of atypical protein kinase C (aPKC) and cell fate determinant Numb, as well as mitotic spindle orientation in neuroblasts. The other player is Drosophila cytoplasmic dynein light chain 1, also named Cut up (Ctp). Ctp localizes at centrioles and controls the mitotic spindle orientation in neuroblasts. Ctp directly interacts with a centriolar protein Anastral Spindle 2 (Ana2) and regulates the localization of Mushroom body defect (Mud). Ana2, Ctp and Mud form a protein complex to regulate spindle orientation in neuroblasts.
author: LI SONG
keywords: neuroblast; self-renewal; asymmetric division; cut up; SCF; Drosophila
date published: 2013-08-06
date modified: 2026-07-24T03:32:18Z
provider: National University of Singapore
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: National University of Singapore