{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/48585"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/48585","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"REGULATION OF RHOGAP DLC1 BY FAK, PP2A AND MEK/ERK IN CELL DYNAMICS","abstract":"DLC1, a functional RhoGAP and tumor suppressor, affects the process of cell migration via its regulation of RhoA. A serine-rich multi-domain protein, DLC1 undergoes post-translational modifications such as phosphorylation. It has been identified as a downstream target for various kinases but effects of growth factors, which play a crucial role in the development and progression of cancer, on DLC1 have not yet been elucidated. We have identified a novel pathway involving the concerted action of Ras/Mek/Erk pathway, Focal adhesion kinase (FAK) and Protein phosphatase-2A (PP2A) to activate DLC1s GAP function. EGF stimulation not only leads to the phosphorylation of DLC1 but also that of FAK to inactivate it, thus allowing PP2A-mediated dephosphorylation at a secondary site on DLC1. This signalling cascade directly affects DLC1s effect on cell spreading and migration, which can be correlated to the reduced RhoA levels.","abstract_html":"DLC1, a functional RhoGAP and tumor suppressor, affects the process of cell migration via its regulation of RhoA. A serine-rich multi-domain protein, DLC1 undergoes post-translational modifications such as phosphorylation. It has been identified as a downstream target for various kinases but effects of growth factors, which play a crucial role in the development and progression of cancer, on DLC1 have not yet been elucidated. We have identified a novel pathway involving the concerted action of Ras/Mek/Erk pathway, Focal adhesion kinase (FAK) and Protein phosphatase-2A (PP2A) to activate DLC1s GAP function. EGF stimulation not only leads to the phosphorylation of DLC1 but also that of FAK to inactivate it, thus allowing PP2A-mediated dephosphorylation at a secondary site on DLC1. This signalling cascade directly affects DLC1s effect on cell spreading and migration, which can be correlated to the reduced RhoA levels.","abstract_has_math":false,"creators":["ARCHNA RAVI"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-08-20","date_published":"2013-08-20","updated_at":"2026-07-24T03:32:04Z","subjects":["DLC1, regulation, cell dynamics"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["ARCHNA RAVI"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2013-08-20"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://scholarbank.nus.edu.sg/handle/10635/48585"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["DLC1, regulation, cell dynamics"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://scholarbank.nus.edu.sg/bitstreams/902b52f0-5bf6-41ad-94cf-0e5a7d8c8717/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["DLC1, a functional RhoGAP and tumor suppressor, affects the process of cell migration via its regulation of RhoA. A serine-rich multi-domain protein, DLC1 undergoes post-translational modifications such as phosphorylation. It has been identified as a downstream target for various kinases but effects of growth factors, which play a crucial role in the development and progression of cancer, on DLC1 have not yet been elucidated. We have identified a novel pathway involving the concerted action of Ras/Mek/Erk pathway, Focal adhesion kinase (FAK) and Protein phosphatase-2A (PP2A) to activate DLC1s GAP function. EGF stimulation not only leads to the phosphorylation of DLC1 but also that of FAK to inactivate it, thus allowing PP2A-mediated dephosphorylation at a secondary site on DLC1. This signalling cascade directly affects DLC1s effect on cell spreading and migration, which can be correlated to the reduced RhoA levels."]},{"key":"dc:format.checksum.md5","label":"Dc Format Checksum Md5","values":["5486d358af2c553081828836c55fd9c4","3e3674b266a9d55b422e40126db9640b"]},{"key":"dc:title","label":"Title","values":["REGULATION OF RHOGAP DLC1 BY FAK, PP2A AND MEK/ERK IN CELL DYNAMICS"]}]}],"canonical_facts":{"dc:creator":["ARCHNA RAVI"],"dc:date.issued":["2013-08-20"],"dc:description.abstract":["DLC1, a functional RhoGAP and tumor suppressor, affects the process of cell migration via its regulation of RhoA. A serine-rich multi-domain protein, DLC1 undergoes post-translational modifications such as phosphorylation. It has been identified as a downstream target for various kinases but effects of growth factors, which play a crucial role in the development and progression of cancer, on DLC1 have not yet been elucidated. We have identified a novel pathway involving the concerted action of Ras/Mek/Erk pathway, Focal adhesion kinase (FAK) and Protein phosphatase-2A (PP2A) to activate DLC1s GAP function. EGF stimulation not only leads to the phosphorylation of DLC1 but also that of FAK to inactivate it, thus allowing PP2A-mediated dephosphorylation at a secondary site on DLC1. This signalling cascade directly affects DLC1s effect on cell spreading and migration, which can be correlated to the reduced RhoA levels."],"dc:format.checksum.md5":["5486d358af2c553081828836c55fd9c4","3e3674b266a9d55b422e40126db9640b"],"dc:identifier.uri":["https://scholarbank.nus.edu.sg/bitstreams/902b52f0-5bf6-41ad-94cf-0e5a7d8c8717/download"],"dc:relation.isreferencedby":["https://scholarbank.nus.edu.sg/handle/10635/48585"],"dc:subject":["DLC1, regulation, cell dynamics"],"dc:title":["REGULATION OF RHOGAP DLC1 BY FAK, PP2A AND MEK/ERK IN CELL DYNAMICS"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T03:32:04Z"}