A TISSUE ENGINEERING APPROACH TO THE DEVELOPMENT OF AN IN VITRO 3D TUMOR MODEL FOR DRUG DELIVERY STUDIES
type: CreativeWork
description: The lack of good preclinical models has hampered anticancer drug discovery. Standard preclinical protocols require the growth of cells in high throughput two-dimensional culture systems. Such in vitro drug testing methods yield drug efficacy results that differ greatly from animal models. It is conceivable that three-dimensional growth may be responsible for some of these changes and that such growth may form a reproducible and more representative step in tumoricidal validation prior to animal implantation. The role of three-dimensional culture on angiogenic potential and tumor growth was studied and high throughput anticancer or anti-angiogenic drug screening was also carried out. A co-culture model with immortalized fibroblasts and endothelial cells was used to study the effects of the supporting stroma on cancer cell behavior. The tumor constructs were cultured in a bioreactor with replicated interstitial fluid pressure, and these constructs were found to closely approximate the subcutaneous tumors in SCID mice.
abstract: The lack of good preclinical models has hampered anticancer drug discovery. Standard preclinical protocols require the growth of cells in high throughput two-dimensional culture systems. Such in vitro drug testing methods yield drug efficacy results that differ greatly from animal models. It is conceivable that three-dimensional growth may be responsible for some of these changes and that such growth may form a reproducible and more representative step in tumoricidal validation prior to animal implantation. The role of three-dimensional culture on angiogenic potential and tumor growth was studied and high throughput anticancer or anti-angiogenic drug screening was also carried out. A co-culture model with immortalized fibroblasts and endothelial cells was used to study the effects of the supporting stroma on cancer cell behavior. The tumor constructs were cultured in a bioreactor with replicated interstitial fluid pressure, and these constructs were found to closely approximate the subcutaneous tumors in SCID mice.
author: TAN HWEE SIM PAMELA
keywords: three-dimensional tumor model, cytotoxicity, angiogenesis, cell cycle arrest, bioreactor, stromal cells
date published: 2012-08-23
date modified: 2026-07-24T03:32:18Z
provider: National University of Singapore
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: National University of Singapore