{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/47630"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/47630","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"A TISSUE ENGINEERING APPROACH TO THE DEVELOPMENT OF AN IN VITRO 3D TUMOR MODEL FOR DRUG DELIVERY STUDIES","abstract":"The lack of good preclinical models has hampered anticancer drug discovery. Standard preclinical protocols require the growth of cells in high throughput two-dimensional culture systems. Such in vitro drug testing methods yield drug efficacy results that differ greatly from animal models. It is conceivable that three-dimensional growth may be responsible for some of these changes and that such growth may form a reproducible and more representative step in tumoricidal validation prior to animal implantation. The role of three-dimensional culture on angiogenic potential and tumor growth was studied and high throughput anticancer or anti-angiogenic drug screening was also carried out. A co-culture model with immortalized fibroblasts and endothelial cells was used to study the effects of the supporting stroma on cancer cell behavior. The tumor constructs were cultured in a bioreactor with replicated interstitial fluid pressure, and these constructs were found to closely approximate the subcutaneous tumors in SCID mice.","abstract_html":"The lack of good preclinical models has hampered anticancer drug discovery. Standard preclinical protocols require the growth of cells in high throughput two-dimensional culture systems. Such in vitro drug testing methods yield drug efficacy results that differ greatly from animal models. It is conceivable that three-dimensional growth may be responsible for some of these changes and that such growth may form a reproducible and more representative step in tumoricidal validation prior to animal implantation. The role of three-dimensional culture on angiogenic potential and tumor growth was studied and high throughput anticancer or anti-angiogenic drug screening was also carried out. A co-culture model with immortalized fibroblasts and endothelial cells was used to study the effects of the supporting stroma on cancer cell behavior. The tumor constructs were cultured in a bioreactor with replicated interstitial fluid pressure, and these constructs were found to closely approximate the subcutaneous tumors in SCID mice.","abstract_has_math":false,"creators":["TAN HWEE SIM PAMELA"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-08-23","date_published":"2012-08-23","updated_at":"2026-07-24T03:32:18Z","subjects":["three-dimensional tumor model, cytotoxicity, angiogenesis, cell cycle arrest, bioreactor, stromal cells"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["TAN HWEE SIM PAMELA"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2012-08-23"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://scholarbank.nus.edu.sg/handle/10635/47630"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["three-dimensional tumor model, cytotoxicity, angiogenesis, cell cycle arrest, bioreactor, stromal cells"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://scholarbank.nus.edu.sg/bitstreams/d8212b63-f77a-457a-b1ef-5b08acc999a7/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The lack of good preclinical models has hampered anticancer drug discovery. Standard preclinical protocols require the growth of cells in high throughput two-dimensional culture systems. Such in vitro drug testing methods yield drug efficacy results that differ greatly from animal models. It is conceivable that three-dimensional growth may be responsible for some of these changes and that such growth may form a reproducible and more representative step in tumoricidal validation prior to animal implantation. The role of three-dimensional culture on angiogenic potential and tumor growth was studied and high throughput anticancer or anti-angiogenic drug screening was also carried out. A co-culture model with immortalized fibroblasts and endothelial cells was used to study the effects of the supporting stroma on cancer cell behavior. The tumor constructs were cultured in a bioreactor with replicated interstitial fluid pressure, and these constructs were found to closely approximate the subcutaneous tumors in SCID mice."]},{"key":"dc:format.checksum.md5","label":"Dc Format Checksum Md5","values":["3e84760179afb75ffae70747fcf5be95","af757a766d99c2f662e7e11396c13235"]},{"key":"dc:title","label":"Title","values":["A TISSUE ENGINEERING APPROACH TO THE DEVELOPMENT OF AN IN VITRO 3D TUMOR MODEL FOR DRUG DELIVERY STUDIES"]}]}],"canonical_facts":{"dc:creator":["TAN HWEE SIM PAMELA"],"dc:date.issued":["2012-08-23"],"dc:description.abstract":["The lack of good preclinical models has hampered anticancer drug discovery. Standard preclinical protocols require the growth of cells in high throughput two-dimensional culture systems. Such in vitro drug testing methods yield drug efficacy results that differ greatly from animal models. It is conceivable that three-dimensional growth may be responsible for some of these changes and that such growth may form a reproducible and more representative step in tumoricidal validation prior to animal implantation. The role of three-dimensional culture on angiogenic potential and tumor growth was studied and high throughput anticancer or anti-angiogenic drug screening was also carried out. A co-culture model with immortalized fibroblasts and endothelial cells was used to study the effects of the supporting stroma on cancer cell behavior. The tumor constructs were cultured in a bioreactor with replicated interstitial fluid pressure, and these constructs were found to closely approximate the subcutaneous tumors in SCID mice."],"dc:format.checksum.md5":["3e84760179afb75ffae70747fcf5be95","af757a766d99c2f662e7e11396c13235"],"dc:identifier.uri":["https://scholarbank.nus.edu.sg/bitstreams/d8212b63-f77a-457a-b1ef-5b08acc999a7/download"],"dc:relation.isreferencedby":["https://scholarbank.nus.edu.sg/handle/10635/47630"],"dc:subject":["three-dimensional tumor model, cytotoxicity, angiogenesis, cell cycle arrest, bioreactor, stromal cells"],"dc:title":["A TISSUE ENGINEERING APPROACH TO THE DEVELOPMENT OF AN IN VITRO 3D TUMOR MODEL FOR DRUG DELIVERY STUDIES"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T03:32:18Z"}